Gene description for MID1
Gene name midline 1
Gene symbol MID1
Other names/aliases BBBG1
FXY
GBBB1
MIDIN
OGS1
OS
OSX
RNF59
TRIM18
XPRF
ZNFXY
Species Homo sapiens
 Database cross references - MID1
ExoCarta ExoCarta_4281
Vesiclepedia VP_4281
Entrez Gene 4281
HGNC 7095
MIM 300552
UniProt O15344  
 MID1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for MID1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IMP
    zinc ion binding GO:0008270 IEA
    transferase activity GO:0016740 IEA
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    phosphoprotein binding GO:0051219 IPI
Biological Process
    microtubule cytoskeleton organization GO:0000226 TAS
    negative regulation of microtubule depolymerization GO:0007026 IEA
    pattern specification process GO:0007389 TAS
    positive regulation of stress-activated MAPK cascade GO:0032874 IMP
    protein localization to microtubule GO:0035372 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IDA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    microtubule associated complex GO:0005875 TAS
    cytoplasmic microtubule GO:0005881 IDA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified MID1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MID1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 PPP2R4 5524
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
3 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SYCE1  
Affinity Capture-MS Homo sapiens
5 MID1IP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 BYSL 705
Two-hybrid Homo sapiens
7 EFTUD1 79631
Co-fractionation Homo sapiens
8 UBC 7316
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 RIC8A 60626
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 STK36  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
11 PTPN1 5770
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 ZNF618  
Two-hybrid Homo sapiens
13 RPS3 6188
Affinity Capture-Western Homo sapiens
14 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBE2K 3093
Two-hybrid Homo sapiens
16 NPM1 4869
Affinity Capture-Western Homo sapiens
17 ZFYVE1 53349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DDRGK1 65992
Affinity Capture-MS Homo sapiens
19 OTUB2  
Two-hybrid Homo sapiens
20 PSMC2 5701
Affinity Capture-MS Homo sapiens
21 MID2 11043
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
22 MLLT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HMG20B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 PSMB6 5694
Affinity Capture-MS Homo sapiens
25 UBE2W  
Two-hybrid Homo sapiens
26 PSMC6 5706
Affinity Capture-MS Homo sapiens
27 FSD2 123722
Affinity Capture-MS Homo sapiens
28 RASGRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 EEF1A1 1915
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 DYRK4  
Two-hybrid Homo sapiens
32 UBE2V1 7335
Two-hybrid Homo sapiens
33 SPAG5 10615
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 UBE2E3 10477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 CDKN2AIP  
Affinity Capture-MS Homo sapiens
36 PSMB4 5692
Affinity Capture-MS Homo sapiens
37 UBE2D4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 PNKP 11284
Co-fractionation Homo sapiens
39 PSMC1 5700
Affinity Capture-MS Homo sapiens
40 PSMD14 10213
Affinity Capture-MS Homo sapiens
41 HTT 3064
Protein-RNA Homo sapiens
42 TUBA1B 10376
Affinity Capture-MS Homo sapiens
43 PSMD2 5708
Affinity Capture-MS Homo sapiens
44 SAR1B 51128
Affinity Capture-MS Homo sapiens
45 PPP2CA 5515
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
46 PKN1 5585
Two-hybrid Homo sapiens
47 CRY2  
Two-hybrid Homo sapiens
48 ZNF784  
Affinity Capture-MS Homo sapiens
49 UBE2D3 7323
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
50 IGBP1 3476
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
51 UBE2N 7334
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
52 PAX6  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 TUBB 203068
Affinity Capture-MS Homo sapiens
54 FAM50B  
Two-hybrid Homo sapiens
55 UBE2L3 7332
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 UBE2L6 9246
Two-hybrid Homo sapiens
57 C19orf66  
Affinity Capture-MS Homo sapiens
58 CUL3 8452
Affinity Capture-MS Homo sapiens
59 TUBB4B 10383
Affinity Capture-MS Homo sapiens
60 UBE2E1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
61 Igbp1  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
62 PSMD7 5713
Affinity Capture-MS Homo sapiens
63 GTF3C4 9329
Affinity Capture-MS Homo sapiens
64 UBE2D2 7322
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
65 MID1 4281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
66 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
67 IRF8  
Affinity Capture-MS Homo sapiens
68 EPN3 55040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 UBTD1 80019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 PSMB5 5693
Affinity Capture-MS Homo sapiens
71 STBD1 8987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TCEANC  
Two-hybrid Homo sapiens
73 GMCL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
74 IL6R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PSMB1 5689
Affinity Capture-MS Homo sapiens
76 GSTT1 2952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 UBE2D1 7321
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
78 MEOX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 EIF1AD 84285
Affinity Capture-MS Homo sapiens
80 PPP4C 5531
Affinity Capture-MS Homo sapiens
81 PDE4B  
Protein-RNA Homo sapiens
82 EHHADH 1962
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
83 CDC37 11140
Two-hybrid Homo sapiens
84 ATG101  
Affinity Capture-MS Homo sapiens
85 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
86 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
87 LRRC4  
Affinity Capture-MS Homo sapiens
88 FKBP1A 2280
Two-hybrid Homo sapiens
89 PSMA1 5682
Affinity Capture-MS Homo sapiens
90 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
91 NQO1 1728
Affinity Capture-MS Homo sapiens
92 RPS8 6202
Affinity Capture-Western Homo sapiens
93 Hmg20b  
Affinity Capture-Western Mus musculus
94 EPHA2 1969
Proximity Label-MS Homo sapiens
95 STT3B 201595
Affinity Capture-MS Homo sapiens
96 GNB2L1 10399
Affinity Capture-Western Homo sapiens
97 AR 367
Affinity Capture-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
98 ANXA2 302
Affinity Capture-Western Homo sapiens
99 ZBTB42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TLDC2  
Affinity Capture-MS Homo sapiens
101 N4BP1  
Two-hybrid Homo sapiens
102 PSMD3 5709
Affinity Capture-MS Homo sapiens
103 UBE2E2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MID1 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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