Gene description for UBE2D1
Gene name ubiquitin-conjugating enzyme E2D 1
Gene symbol UBE2D1
Other names/aliases E2(17)KB1
SFT
UBC4/5
UBCH5
UBCH5A
Species Homo sapiens
 Database cross references - UBE2D1
ExoCarta ExoCarta_7321
Vesiclepedia VP_7321
Entrez Gene 7321
HGNC 12474
MIM 602961
UniProt P51668  
 UBE2D1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for UBE2D1
Molecular Function
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ligase activity GO:0016874 IEA
Biological Process
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    mitotic spindle assembly checkpoint GO:0007094 TAS
    cellular response to hypoxia GO:0071456 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    protein K48-linked ubiquitination GO:0070936 IDA
    innate immune response GO:0045087 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    transcription, DNA-templated GO:0006351 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein polyubiquitination GO:0000209 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    gene expression GO:0010467 TAS
    mitotic cell cycle GO:0000278 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    BMP signaling pathway GO:0030509 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    positive regulation of protein ubiquitination GO:0031398 IDA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
Subcellular Localization
    protein complex GO:0043234 IDA
    cytoplasm GO:0005737 IDA
    ubiquitin ligase complex GO:0000151 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified UBE2D1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBE2D1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 BRCC3 79184
Reconstituted Complex Homo sapiens
3 RNF2  
Biochemical Activity Homo sapiens
4 BRCA1 672
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 MDM4  
Reconstituted Complex Homo sapiens
6 STUB1  
Affinity Capture-MS Homo sapiens
7 DTX3L 151636
Affinity Capture-Western Homo sapiens
8 FANCD2  
Reconstituted Complex Homo sapiens
9 BARD1 580
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 RNF126  
Two-hybrid Homo sapiens
11 UBE3A 7337
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
12 Ubox5  
Two-hybrid Mus musculus
13 BRE 9577
Reconstituted Complex Homo sapiens
14 TRIM39  
Two-hybrid Homo sapiens
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