Gene description for KLHL13
Gene name kelch-like family member 13
Gene symbol KLHL13
Other names/aliases BKLHD2
Species Homo sapiens
 Database cross references - KLHL13
ExoCarta ExoCarta_90293
Vesiclepedia VP_90293
Entrez Gene 90293
HGNC 22931
MIM 300655
UniProt Q9P2N7  
 KLHL13 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KLHL13
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    cullin family protein binding GO:0097602 IBA
Biological Process
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    regulation of cytokinesis GO:0032465 IBA
    regulation of cytokinesis GO:0032465 IMP
    cell division GO:0051301 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
 Experiment description of studies that identified KLHL13 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KLHL13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 LY86  
Affinity Capture-MS Homo sapiens
3 ZMYM4  
Affinity Capture-MS Homo sapiens
4 C1QTNF3 114899
Affinity Capture-MS Homo sapiens
5 ZNF414  
Affinity Capture-MS Homo sapiens
6 KLHL28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PARD3B 117583
Affinity Capture-MS Homo sapiens
8 NXT2  
Affinity Capture-MS Homo sapiens
9 POLR2M  
Affinity Capture-MS Homo sapiens
10 KLHL15  
Affinity Capture-MS Homo sapiens
11 GJA3  
Affinity Capture-MS Homo sapiens
12 GIPC1 10755
Affinity Capture-MS Homo sapiens
13 ETV6  
Affinity Capture-MS Homo sapiens
14 MAD2L1 4085
Affinity Capture-MS Homo sapiens
15 COPS7A 50813
Affinity Capture-MS Homo sapiens
16 Cd2ap 12488
Affinity Capture-MS Mus musculus
17 PCDHGA5  
Affinity Capture-MS Homo sapiens
18 VIP  
Affinity Capture-MS Homo sapiens
19 Mad2l1 56150
Affinity Capture-MS Mus musculus
20 KIFC3 3801
Affinity Capture-MS Homo sapiens
21 HEXIM2  
Affinity Capture-MS Homo sapiens
22 KIRREL2  
Affinity Capture-MS Homo sapiens
23 C9orf72  
Affinity Capture-MS Homo sapiens
24 UBXN7 26043
Affinity Capture-MS Homo sapiens
25 NOG 9241
Affinity Capture-MS Homo sapiens
26 NHLRC2 374354
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 NUDCD3 23386
Affinity Capture-MS Homo sapiens
28 MCFD2 90411
Affinity Capture-MS Homo sapiens
29 CUL3 8452
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 ZNF444  
Affinity Capture-MS Homo sapiens
31 Cul3  
Affinity Capture-MS Mus musculus
32 UBC 7316
Reconstituted Complex Homo sapiens
33 USP11 8237
Affinity Capture-MS Homo sapiens
34 USP25  
Affinity Capture-MS Homo sapiens
35 CSPP1  
Affinity Capture-MS Homo sapiens
36 TMEM44  
Affinity Capture-MS Homo sapiens
37 IGFBP4 3487
Affinity Capture-MS Homo sapiens
38 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
39 SOX9  
Affinity Capture-Western Homo sapiens
40 FAM219B 57184
Affinity Capture-MS Homo sapiens
41 LURAP1L  
Affinity Capture-MS Homo sapiens
42 TFG 10342
Affinity Capture-MS Homo sapiens
43 TMEM171  
Affinity Capture-MS Homo sapiens
44 PPP1R16B  
Affinity Capture-MS Homo sapiens
45 IGLV6-57  
Affinity Capture-MS Homo sapiens
46 AURKB 9212
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
47 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 COPS8 10920
Affinity Capture-MS Homo sapiens
49 SYT3  
Affinity Capture-MS Homo sapiens
50 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
51 GPS1 2873
Affinity Capture-MS Homo sapiens
52 MPI 4351
Affinity Capture-MS Homo sapiens
53 HEXIM1 10614
Affinity Capture-MS Homo sapiens
54 Klhl21  
Affinity Capture-MS Mus musculus
55 FIBIN  
Affinity Capture-MS Homo sapiens
56 ZMYM6  
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 COPS6 10980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MZB1  
Affinity Capture-MS Homo sapiens
60 GNLY  
Affinity Capture-MS Homo sapiens
61 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TPST2 8459
Affinity Capture-MS Homo sapiens
63 RBM11  
Affinity Capture-MS Homo sapiens
64 SCRIB 23513
Affinity Capture-MS Homo sapiens
65 COPS2 9318
Affinity Capture-MS Homo sapiens
66 Kctd5  
Affinity Capture-MS Mus musculus
67 GPRC5B 51704
Affinity Capture-MS Homo sapiens
68 TRIM29 23650
Affinity Capture-MS Homo sapiens
69 C1qbp 12261
Affinity Capture-MS Mus musculus
70 PRPS1 5631
Affinity Capture-MS Homo sapiens
71 RPA2 6118
Proximity Label-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 FBXL17  
Affinity Capture-MS Homo sapiens
74 KLHL21 9903
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
75 BHLHE23  
Affinity Capture-MS Homo sapiens
76 ENC1  
Affinity Capture-MS Homo sapiens
77 COPS4 51138
Affinity Capture-MS Homo sapiens
78 KLHL14 57565
Affinity Capture-MS Homo sapiens
79 UBE2D1 7321
Reconstituted Complex Homo sapiens
80 Klhl22  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here