Gene description for KLHL22
Gene name kelch-like family member 22
Gene symbol KLHL22
Other names/aliases KELCHL
Species Homo sapiens
 Database cross references - KLHL22
ExoCarta ExoCarta_84861
Vesiclepedia VP_84861
Entrez Gene 84861
HGNC 25888
UniProt Q53GT1  
 KLHL22 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KLHL22
Molecular Function
    protein binding GO:0005515 IPI
    14-3-3 protein binding GO:0071889 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    mitotic sister chromatid segregation GO:0000070 IMP
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein monoubiquitination GO:0006513 IBA
    protein monoubiquitination GO:0006513 IDA
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    negative regulation of autophagy GO:0010507 IMP
    positive regulation of cell growth GO:0030307 IMP
    negative regulation of type I interferon production GO:0032480 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    positive regulation of T cell activation GO:0050870 IDA
    cell division GO:0051301 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    cellular response to L-leucine GO:0071233 IMP
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IDA
    positive regulation of TORC1 signaling GO:1904263 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IC
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    centrosome GO:0005813 IDA
    polar microtubule GO:0005827 IBA
    polar microtubule GO:0005827 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule cytoskeleton GO:0015630 IDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    intercellular bridge GO:0045171 IDA
    mitotic spindle GO:0072686 IBA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified KLHL22 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KLHL22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FCN1 2219
Affinity Capture-MS Homo sapiens
3 LYRM2  
Affinity Capture-MS Homo sapiens
4 POLDIP3 84271
Affinity Capture-MS Homo sapiens
5 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SAV1  
Affinity Capture-MS Homo sapiens
7 ZBTB34 403341
Affinity Capture-MS Homo sapiens
8 RBM15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PDCD1  
Affinity Capture-MS Homo sapiens
10 NUDCD3 23386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NOA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MRPL10 124995
Affinity Capture-MS Homo sapiens
13 TRMT6 51605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 KLRB1  
Affinity Capture-MS Homo sapiens
15 RPUSD4 84881
Affinity Capture-MS Homo sapiens
16 SOX2  
Affinity Capture-MS Homo sapiens
17 ATG4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SCARF2 91179
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 C7orf55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TMEM154  
Affinity Capture-MS Homo sapiens
21 PLOD3 8985
Affinity Capture-MS Homo sapiens
22 ZBTB33  
Affinity Capture-MS Homo sapiens
23 APOC3 345
Affinity Capture-MS Homo sapiens
24 COPS6 10980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GKN1  
Affinity Capture-MS Homo sapiens
26 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 POLRMT 5442
Affinity Capture-MS Homo sapiens
28 FAF1 11124
Affinity Capture-MS Homo sapiens
29 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 LYRM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PRTN3 5657
Affinity Capture-MS Homo sapiens
32 TMEM87A 25963
Affinity Capture-MS Homo sapiens
33 ADRA1A  
Two-hybrid Homo sapiens
34 FAM163A  
Affinity Capture-MS Homo sapiens
35 EMC1 23065
Affinity Capture-MS Homo sapiens
36 AGFG1 3267
Affinity Capture-MS Homo sapiens
37 MICU2 221154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NDUFAF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 LYRM7  
Affinity Capture-MS Homo sapiens
41 BMP7 655
Affinity Capture-MS Homo sapiens
42 CLEC11A 6320
Affinity Capture-MS Homo sapiens
43 C4BPB 725
Affinity Capture-MS Homo sapiens
44 IGLV6-57  
Affinity Capture-MS Homo sapiens
45 NOB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 LYRM4 57128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MICU1 10367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KLHL14 57565
Affinity Capture-MS Homo sapiens
49 GLUD2 2747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GFER  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MAP7 9053
Affinity Capture-MS Homo sapiens
52 IGHA1 3493
Affinity Capture-MS Homo sapiens
53 ZG16B 124220
Affinity Capture-MS Homo sapiens
54 UBXN7 26043
Affinity Capture-MS Homo sapiens
55 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 NFS1 9054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
58 DES 1674
Affinity Capture-MS Homo sapiens
59 KISS1  
Affinity Capture-MS Homo sapiens
60 PLK1 5347
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
61 KLHL13 90293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SYDE1 85360
Affinity Capture-MS Homo sapiens
63 AMY1C 278
Affinity Capture-MS Homo sapiens
64 SCAF4 57466
Affinity Capture-MS Homo sapiens
65 TRIM32 22954
Affinity Capture-MS Homo sapiens
66 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
67 RPP25  
Affinity Capture-MS Homo sapiens
68 IGFBP4 3487
Affinity Capture-MS Homo sapiens
69 BICD2 23299
Proximity Label-MS Homo sapiens
70 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
71 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CCL21  
Affinity Capture-MS Homo sapiens
73 ATPAF2 91647
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 LCN1 3933
Affinity Capture-MS Homo sapiens
75 BMP1 649
Affinity Capture-MS Homo sapiens
76 METTL15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 GPA33 10223
Affinity Capture-MS Homo sapiens
78 PLA2G1B  
Affinity Capture-MS Homo sapiens
79 UBXN1 51035
Affinity Capture-MS Homo sapiens
80 RBM11  
Affinity Capture-MS Homo sapiens
81 GBAS 2631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SHKBP1  
Affinity Capture-MS Homo sapiens
83 MZB1  
Affinity Capture-MS Homo sapiens
84 GLUD1 2746
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 RBFA  
Affinity Capture-MS Homo sapiens
86 PDGFD 80310
Affinity Capture-MS Homo sapiens
87 CTHRC1 115908
Affinity Capture-MS Homo sapiens
88 PDCD2L 84306
Affinity Capture-MS Homo sapiens
89 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 KLHL26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 HBA2 3040
Affinity Capture-MS Homo sapiens
92 CILP2  
Affinity Capture-MS Homo sapiens
93 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
94 IPP  
Affinity Capture-MS Homo sapiens
95 NDUFB9 4715
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SRGAP1 57522
Affinity Capture-MS Homo sapiens
97 REG3A  
Affinity Capture-MS Homo sapiens
98 PNKD  
Affinity Capture-MS Homo sapiens
99 NACC2  
Affinity Capture-MS Homo sapiens
100 REG4 83998
Affinity Capture-MS Homo sapiens
101 SCAF8 22828
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 CIZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 MATN2 4147
Affinity Capture-MS Homo sapiens
104 SPINT2 10653
Affinity Capture-MS Homo sapiens
105 PAEP  
Affinity Capture-MS Homo sapiens
106 NEDD8 4738
Affinity Capture-MS Homo sapiens
107 CTNND1 1500
Affinity Capture-MS Homo sapiens
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