Gene description for SCAF4
Gene name SR-related CTD-associated factor 4
Gene symbol SCAF4
Other names/aliases SFRS15
SRA4
Species Homo sapiens
 Database cross references - SCAF4
ExoCarta ExoCarta_57466
Vesiclepedia VP_57466
Entrez Gene 57466
HGNC 19304
MIM 616023
UniProt O95104  
 SCAF4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SCAF4
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA polymerase II C-terminal domain phosphoserine binding GO:1990269 IBA
    RNA polymerase II C-terminal domain phosphoserine binding GO:1990269 IDA
Biological Process
    negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled GO:2000805 IBA
    negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled GO:2000805 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified SCAF4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SCAF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2G 5436
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 PRM2  
Affinity Capture-MS Homo sapiens
4 UQCRFS1 7386
Co-fractionation Homo sapiens
5 OXCT1 5019
Co-fractionation Homo sapiens
6 RNGTT 8732
Affinity Capture-MS Homo sapiens
7 MED17  
Affinity Capture-MS Homo sapiens
8 CLEC3A  
Affinity Capture-MS Homo sapiens
9 ZNF346  
Affinity Capture-MS Homo sapiens
10 CCNB2  
Affinity Capture-MS Homo sapiens
11 SRPK2 6733
Affinity Capture-MS Homo sapiens
12 RPAP2  
Affinity Capture-MS Homo sapiens
13 RPA2 6118
Affinity Capture-MS Homo sapiens
14 ACTC1 70
Proximity Label-MS Homo sapiens
15 C1orf189  
Affinity Capture-MS Homo sapiens
16 PRPF40A 55660
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
17 SNRPF 6636
Affinity Capture-MS Homo sapiens
18 RPL4 6124
Affinity Capture-MS Homo sapiens
19 RNPS1 10921
Affinity Capture-MS Homo sapiens
20 SLC13A2 9058
Affinity Capture-MS Homo sapiens
21 CHCHD2  
Affinity Capture-MS Homo sapiens
22 POLR2I 5438
Affinity Capture-MS Homo sapiens
23 CDK12 51755
Co-fractionation Homo sapiens
24 SNRPC 6631
Affinity Capture-MS Homo sapiens
25 MED27  
Affinity Capture-MS Homo sapiens
26 OBSL1 23363
Affinity Capture-MS Homo sapiens
27 EED  
Affinity Capture-MS Homo sapiens
28 POLR2C 5432
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
29 GPN1  
Affinity Capture-MS Homo sapiens
30 POLR2M  
Affinity Capture-MS Homo sapiens
31 SH3BGRL 6451
Co-fractionation Homo sapiens
32 MED4 29079
Affinity Capture-MS Homo sapiens
33 POLR2K  
Affinity Capture-MS Homo sapiens
34 MED1 5469
Affinity Capture-MS Homo sapiens
35 WBP4  
Reconstituted Complex Homo sapiens
36 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SCAF1  
Affinity Capture-MS Homo sapiens
38 MED20 9477
Affinity Capture-MS Homo sapiens
39 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
40 QPRT 23475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TXNDC17 84817
Co-fractionation Homo sapiens
42 SUPT5H 6829
Affinity Capture-MS Homo sapiens
43 SMEK1 55671
Affinity Capture-MS Homo sapiens
44 ACIN1 22985
Cross-Linking-MS (XL-MS) Homo sapiens
45 HLX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 VCL 7414
Co-fractionation Homo sapiens
47 GATA3  
Proximity Label-MS Homo sapiens
48 TNPO3 23534
Affinity Capture-MS Homo sapiens
49 MED26  
Affinity Capture-MS Homo sapiens
50 RAD18  
Affinity Capture-MS Homo sapiens
51 RPL36 25873
Affinity Capture-MS Homo sapiens
52 COA7  
Co-fractionation Homo sapiens
53 VMA21  
Co-fractionation Homo sapiens
54 RPLP0 6175
Affinity Capture-MS Homo sapiens
55 MAGEB2 4113
Affinity Capture-MS Homo sapiens
56 SPTY2D1  
Co-fractionation Homo sapiens
57 CEBPA  
Protein-peptide Homo sapiens
58 FGF13  
Affinity Capture-MS Homo sapiens
59 BARD1 580
Affinity Capture-MS Homo sapiens
60 POLR2B 5431
Affinity Capture-MS Homo sapiens
61 RECQL5  
Affinity Capture-MS Homo sapiens
62 VAPB 9217
Co-fractionation Homo sapiens
63 TOP1 7150
Affinity Capture-MS Homo sapiens
64 POLR2H 5437
Affinity Capture-MS Homo sapiens
65 C9orf78 51759
Affinity Capture-MS Homo sapiens
66 PSENEN  
Affinity Capture-MS Homo sapiens
67 SNRPB 6628
Affinity Capture-MS Homo sapiens
68 POLR2E 5434
Affinity Capture-MS Homo sapiens
69 RPRD2  
Affinity Capture-MS Homo sapiens
70 MECP2 4204
Affinity Capture-MS Homo sapiens
71 RPRD1A  
Affinity Capture-MS Homo sapiens
72 POLR2D  
Affinity Capture-MS Homo sapiens
73 TRIM68 55128
Affinity Capture-MS Homo sapiens
74 FUS 2521
Affinity Capture-MS Homo sapiens
75 POLR2A 5430
Affinity Capture-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 MED10  
Affinity Capture-MS Homo sapiens
78 HNRNPA1 3178
Proximity Label-MS Homo sapiens
79 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
80 SELENBP1 8991
Co-fractionation Homo sapiens
81 TP63  
Two-hybrid Homo sapiens
82 RPL35A 6165
Affinity Capture-MS Homo sapiens
83 DNAJC10 54431
Cross-Linking-MS (XL-MS) Homo sapiens
84 CTNNA1 1495
Cross-Linking-MS (XL-MS) Homo sapiens
85 H2AFB2  
Affinity Capture-MS Homo sapiens
86 UQCRFS1P1  
Co-fractionation Homo sapiens
87 MED14  
Affinity Capture-MS Homo sapiens
88 SUMO1 7341
Affinity Capture-MS Homo sapiens
89 ZNF287  
Affinity Capture-MS Homo sapiens
90 MED19  
Affinity Capture-MS Homo sapiens
91 POLR2J  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 PRKCSH 5589
Cross-Linking-MS (XL-MS) Homo sapiens
93 POLR2F  
Affinity Capture-MS Homo sapiens
94 SVIL 6840
Co-fractionation Homo sapiens
95 CPSF6 11052
Affinity Capture-MS Homo sapiens
96 RRS1 23212
Affinity Capture-MS Homo sapiens
97 WWOX 51741
Affinity Capture-MS Homo sapiens
98 POLR2L 5441
Affinity Capture-MS Homo sapiens
99 SUCLG2 8801
Co-fractionation Homo sapiens
100 RPA1 6117
Affinity Capture-MS Homo sapiens
101 SNRPA 6626
Affinity Capture-MS Homo sapiens
102 MED8  
Affinity Capture-MS Homo sapiens
103 MED6  
Affinity Capture-MS Homo sapiens
104 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
105 H2AFB3  
Affinity Capture-MS Homo sapiens
106 MED21  
Affinity Capture-MS Homo sapiens
107 SUPT6H 6830
Affinity Capture-MS Homo sapiens
108 GAGE5  
Affinity Capture-MS Homo sapiens
109 RPRD1B 58490
Affinity Capture-MS Homo sapiens
110 KLHL22 84861
Affinity Capture-MS Homo sapiens
111 VDAC3 7419
Co-fractionation Homo sapiens
112 SSRP1 6749
Affinity Capture-MS Homo sapiens
113 PLAUR 5329
Co-fractionation Homo sapiens
114 SP7  
Proximity Label-MS Homo sapiens
115 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 EP300 2033
Affinity Capture-MS Homo sapiens
117 MED28  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SCAF4 is involved
No pathways found





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