Experiment description of studies that identified PRPF40A in exosomes

1 Experiment ID 207 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 ISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 226 ISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✔ CD9|FLOT1 EV Membrane markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 EV Negative markers ✘ EV Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

Protein-protein interactions for PRPF40A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PCBP2 5094 Affinity Capture-MS Homo sapiens 2 MECP2 4204 Affinity Capture-MS Homo sapiens Far Western Homo sapiens 3 FNBP4   Affinity Capture-MS Homo sapiens 4 FMNL1 752 Affinity Capture-MS Homo sapiens 5 UBQLN4 56893 Affinity Capture-MS Homo sapiens 6 SERPINB9 5272 Two-hybrid Homo sapiens 7 PSG5   Affinity Capture-MS Homo sapiens 8 PRKAR1A 5573 Affinity Capture-MS Homo sapiens 9 FASLG 356 Invivo Homo sapiens 10 RNU2   Affinity Capture-RNA Homo sapiens 11 SF1 7536 Affinity Capture-MS Homo sapiens 12 SMAD4   Affinity Capture-MS Homo sapiens 13 PIAS3   Affinity Capture-MS Homo sapiens 14 PRPF40A 55660 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 HNRNPUL1 11100 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 16 FMNL3 91010 Far Western Homo sapiens 17 WBP5   Far Western Homo sapiens 18 BAG6 7917 Affinity Capture-MS Homo sapiens 19 HTT 3064 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 20 UBE2I 7329 Affinity Capture-MS Homo sapiens 21 ATBF1   Affinity Capture-MS Homo sapiens Far Western Homo sapiens 22 CDK13 8621 Affinity Capture-MS Homo sapiens 23 RNPS1 10921 Affinity Capture-MS Homo sapiens 24 PFKL 5211 Affinity Capture-MS Homo sapiens 25 VCL 7414 Affinity Capture-MS Homo sapiens 26 U2AF2 11338 Affinity Capture-MS Homo sapiens 27 HIPK1 204851 Affinity Capture-MS Homo sapiens 28 FMNL2 114793 Affinity Capture-MS Homo sapiens 29 UBQLN1 29979 Affinity Capture-MS Homo sapiens 30 ZNF451 26036 Affinity Capture-MS Homo sapiens 31 CAP2   Affinity Capture-MS Homo sapiens 32 RNU1-4 6060 Affinity Capture-RNA Homo sapiens View the network

Pathways in which PRPF40A is involved

No pathways found