Gene description for ARFGEF2
Gene name ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Gene symbol ARFGEF2
Other names/aliases BIG2
PVNH2
dJ1164I10.1
Species Homo sapiens
 Database cross references - ARFGEF2
ExoCarta ExoCarta_10564
Vesiclepedia VP_10564
Entrez Gene 10564
HGNC 15853
MIM 605371
UniProt Q9Y6D5  
 ARFGEF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Endothelial cells 26027894    
 Gene ontology annotations for ARFGEF2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    myosin binding GO:0017022 IEA
    protein kinase A regulatory subunit binding GO:0034237 IDA
    GABA receptor binding GO:0050811 ISS
Biological Process
    receptor recycling GO:0001881 IDA
    exocytosis GO:0006887 IEA
    Golgi to plasma membrane transport GO:0006893 IMP
    endosome organization GO:0007032 IMP
    endomembrane system organization GO:0010256 IMP
    protein transport GO:0015031 IEA
    regulation of ARF protein signal transduction GO:0032012 IEA
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    intracellular signal transduction GO:0035556 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 ISS
    centrosome GO:0005813 IEA
    microtubule organizing center GO:0005815 IDA
    cytosol GO:0005829 IDA
    axonemal microtubule GO:0005879 ISS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    cytoplasmic vesicle GO:0031410 ISS
    asymmetric synapse GO:0032279 ISS
    symmetric synapse GO:0032280 ISS
    dendritic spine GO:0043197 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    recycling endosome GO:0055037 IDA
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified ARFGEF2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
11
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for ARFGEF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCA  
Affinity Capture-MS Homo sapiens
2 FAM131B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 AKAP10 11216
Affinity Capture-MS Homo sapiens
4 LAMP3  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HLA-E 3133
Affinity Capture-MS Homo sapiens
6 KRT73 319101
Affinity Capture-MS Homo sapiens
7 PDCD1  
Affinity Capture-MS Homo sapiens
8 DNAJB2 3300
Affinity Capture-MS Homo sapiens
9 PRMT6  
Affinity Capture-MS Homo sapiens
10 FPR1  
Affinity Capture-MS Homo sapiens
11 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 EDNRB 1910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 KLRB1  
Affinity Capture-MS Homo sapiens
15 PSMD4 5710
Affinity Capture-MS Homo sapiens
16 PRPF40A 55660
Two-hybrid Homo sapiens
17 ANKMY2 57037
Co-fractionation Homo sapiens
18 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MRPL23 6150
Affinity Capture-MS Homo sapiens
20 KCNH4  
Affinity Capture-MS Homo sapiens
21 CLEC2B  
Affinity Capture-MS Homo sapiens
22 STX7 8417
Proximity Label-MS Homo sapiens
23 PSMC6 5706
Affinity Capture-MS Homo sapiens
24 STX6 10228
Proximity Label-MS Homo sapiens
25 ITGA8 8516
Affinity Capture-MS Homo sapiens
26 ITFG3 83986
Affinity Capture-MS Homo sapiens
27 LDLRAD4  
Affinity Capture-MS Homo sapiens
28 RAB35 11021
Proximity Label-MS Homo sapiens
29 CSNK2B 1460
Affinity Capture-MS Homo sapiens
30 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NHLH2  
Affinity Capture-MS Homo sapiens
32 GPR17 2840
Affinity Capture-MS Homo sapiens
33 LAMTOR1 55004
Proximity Label-MS Homo sapiens
34 VSIG4  
Affinity Capture-MS Homo sapiens
35 BSG 682
Affinity Capture-MS Homo sapiens
36 CTDP1 9150
Affinity Capture-MS Homo sapiens
37 RAB4A 5867
Proximity Label-MS Homo sapiens
38 DTNBP1  
Affinity Capture-MS Homo sapiens
39 PBXIP1 57326
Affinity Capture-MS Homo sapiens
40 PSMD2 5708
Affinity Capture-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
42 GPR182  
Affinity Capture-MS Homo sapiens
43 RAB7A 7879
Proximity Label-MS Homo sapiens
44 PRKAR2A 5576
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
45 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ELOVL5 60481
Proximity Label-MS Homo sapiens
48 HSPB8 26353
Affinity Capture-MS Homo sapiens
49 UPK2 7379
Affinity Capture-MS Homo sapiens
50 CD40 958
Affinity Capture-MS Homo sapiens
51 NUP88 4927
Co-fractionation Homo sapiens
52 CYB5B 80777
Affinity Capture-MS Homo sapiens
53 CDC73  
Co-fractionation Homo sapiens
54 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ICAM1 3383
Affinity Capture-MS Homo sapiens
56 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PSMD13 5719
Affinity Capture-MS Homo sapiens
58 LRRC25  
Affinity Capture-MS Homo sapiens
59 NPTN 27020
Affinity Capture-MS Homo sapiens
60 ARFGEF1 10565
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
61 PRKAR1A 5573
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
62 MGARP  
Affinity Capture-MS Homo sapiens
63 CUL3 8452
Affinity Capture-MS Homo sapiens
64 CLEC4A  
Affinity Capture-MS Homo sapiens
65 MADCAM1  
Affinity Capture-MS Homo sapiens
66 SPACA1 81833
Affinity Capture-MS Homo sapiens
67 OCIAD1 54940
Affinity Capture-MS Homo sapiens
68 C3orf18  
Affinity Capture-MS Homo sapiens
69 MYC  
Dosage Lethality Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 RAB9A 9367
Proximity Label-MS Homo sapiens
72 CD244 51744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 EBAG9 9166
Proximity Label-MS Homo sapiens
74 HAX1  
Affinity Capture-MS Homo sapiens
75 PNPLA2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
76 KRT16 3868
Affinity Capture-MS Homo sapiens
77 MYCBP 26292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
79 STK16 8576
Affinity Capture-MS Homo sapiens
80 TMEM9 252839
Affinity Capture-MS Homo sapiens
81 OPALIN  
Affinity Capture-MS Homo sapiens
82 MFSD4  
Affinity Capture-MS Homo sapiens
83 CLEC2D  
Affinity Capture-MS Homo sapiens
84 DPY30 84661
Affinity Capture-MS Homo sapiens
85 Itch 16396
Reconstituted Complex Mus musculus
86 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 NUP98 4928
Co-fractionation Homo sapiens
88 TMEM171  
Affinity Capture-MS Homo sapiens
89 TUBB6 84617
Affinity Capture-MS Homo sapiens
90 GZMH  
Affinity Capture-MS Homo sapiens
91 FLNA 2316
Co-localization Homo sapiens
92 RAB11A 8766
Proximity Label-MS Homo sapiens
93 ORAI1 84876
Affinity Capture-MS Homo sapiens
94 FLNC 2318
Co-fractionation Homo sapiens
95 SNW1 22938
Affinity Capture-MS Homo sapiens
96 PAF1 54623
Co-fractionation Homo sapiens
97 VASN 114990
Affinity Capture-MS Homo sapiens
98 PSMD3 5709
Affinity Capture-MS Homo sapiens
99 FKBP2 2286
Affinity Capture-Western Homo sapiens
100 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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