Gene description for ARFGEF1
Gene name ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Gene symbol ARFGEF1
Other names/aliases ARFGEP1
BIG1
P200
Species Homo sapiens
 Database cross references - ARFGEF1
ExoCarta ExoCarta_10565
Vesiclepedia VP_10565
Entrez Gene 10565
HGNC 15772
MIM 604141
UniProt Q9Y6D6  
 ARFGEF1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARFGEF1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    protein kinase A regulatory subunit binding GO:0034237 IDA
    ARF guanyl-nucleotide exchange factor activity GO:0005086 IBA
    myosin binding GO:0017022 IPI
    GTPase activator activity GO:0005096 IDA
Biological Process
    endomembrane system organization GO:0010256 IMP
    positive regulation of protein glycosylation in Golgi GO:0090284 IMP
    negative regulation of GTPase activity GO:0034260 IDA
    positive regulation of wound healing GO:0090303 IMP
    exocytosis GO:0006887 TAS
    negative regulation of actin filament polymerization GO:0030837 IMP
    positive regulation of GTPase activity GO:0043547 IEA
    regulation of establishment of cell polarity GO:2000114 IMP
    Golgi organization GO:0007030 IMP
    protein transport GO:0015031 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    small nuclear ribonucleoprotein complex GO:0030532 IDA
    nuclear matrix GO:0016363 IEA
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 IDA
    nucleolus GO:0005730 IDA
    Golgi membrane GO:0000139 IDA
 Experiment description of studies that identified ARFGEF1 in exosomes
1
Experiment ID 1203
MISEV standards
EM
EV Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARFGEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAR1A 5573
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 ARFGEF2 10564
Invivo Homo sapiens
3 FKBP2 2286
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ARFGEF1 is involved
No pathways found





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