Gene description for DENND6A
Gene name DENN/MADD domain containing 6A
Gene symbol DENND6A
Other names/aliases AFI1A
FAM116A
Species Homo sapiens
 Database cross references - DENND6A
ExoCarta ExoCarta_201627
Vesiclepedia VP_201627
Entrez Gene 201627
HGNC 26635
UniProt Q8IWF6  
 DENND6A identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DENND6A
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified DENND6A in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DENND6A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DST 667
Proximity Label-MS Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 PRKCSH 5589
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DSG2 1829
Proximity Label-MS Homo sapiens
5 CCDC88A 55704
Proximity Label-MS Homo sapiens
6 HIST1H3F 8968
Proximity Label-MS Homo sapiens
7 MAP7 9053
Proximity Label-MS Homo sapiens
8 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RAB11FIP5 26056
Proximity Label-MS Homo sapiens
10 MAS1  
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 LAMP3  
Proximity Label-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NBEA  
Proximity Label-MS Homo sapiens
17 HCST  
Affinity Capture-MS Homo sapiens
18 WDR7 23335
Proximity Label-MS Homo sapiens
19 EML3 256364
Affinity Capture-MS Homo sapiens
20 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
21 CADM4 199731
Affinity Capture-MS Homo sapiens
22 RAB9A 9367
Proximity Label-MS Homo sapiens
23 LAMP1 3916
Proximity Label-MS Homo sapiens
24 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ARFGEF1 10565
Proximity Label-MS Homo sapiens
26 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RABEP2 79874
Affinity Capture-MS Homo sapiens
28 CX3CL1  
Affinity Capture-MS Homo sapiens
29 LMTK2 22853
Proximity Label-MS Homo sapiens
30 EFNB1 1947
Affinity Capture-MS Homo sapiens
31 LYRM1  
Co-fractionation Homo sapiens
32 LAMP2 3920
Proximity Label-MS Homo sapiens
33 CAMSAP2  
Proximity Label-MS Homo sapiens
34 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 VPS51 738
Proximity Label-MS Homo sapiens
36 KIAA1468 57614
Proximity Label-MS Homo sapiens
37 LTN1 26046
Proximity Label-MS Homo sapiens
38 PSME1 5720
Affinity Capture-MS Homo sapiens
39 HIST1H4J 8363
Proximity Label-MS Homo sapiens
40 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PLEKHA5 54477
Proximity Label-MS Homo sapiens
42 VSIG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SCAMP1 9522
Proximity Label-MS Homo sapiens
44 UBB 7314
Proximity Label-MS Homo sapiens
45 RAB11B 9230
Proximity Label-MS Homo sapiens
46 GCH1  
Affinity Capture-MS Homo sapiens
47 RAB2A 5862
Proximity Label-MS Homo sapiens
48 BTN2A1 11120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CTLA4  
Affinity Capture-MS Homo sapiens
50 SIPA1L2  
Proximity Label-MS Homo sapiens
51 RAB11A 8766
Proximity Label-MS Homo sapiens
52 SSSCA1 10534
Affinity Capture-MS Homo sapiens
53 DENND6B  
Affinity Capture-MS Homo sapiens
54 DSC2 1824
Proximity Label-MS Homo sapiens
55 EPHA2 1969
Proximity Label-MS Homo sapiens
56 RPS6KC1  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LRBA 987
Proximity Label-MS Homo sapiens
58 PTGES3 10728
Affinity Capture-MS Homo sapiens
59 RAB5C 5878
Proximity Label-MS Homo sapiens
60 ACVR2B  
Affinity Capture-MS Homo sapiens
61 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SLC31A1 1317
Affinity Capture-MS Homo sapiens
63 ARL13B 200894
Proximity Label-MS Homo sapiens
64 TBC1D10B 26000
Proximity Label-MS Homo sapiens
65 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 EFNB3 1949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HSPA1B 3304
Proximity Label-MS Homo sapiens
69 SPRYD7 57213
Affinity Capture-MS Homo sapiens
70 LRP8 7804
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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