Gene description for CADM4
Gene name cell adhesion molecule 4
Gene symbol CADM4
Other names/aliases IGSF4C
NECL4
Necl-4
TSLL2
synCAM4
Species Homo sapiens
 Database cross references - CADM4
ExoCarta ExoCarta_199731
Vesiclepedia VP_199731
Entrez Gene 199731
HGNC 30825
MIM 609744
UniProt Q8NFZ8  
 CADM4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
 Gene ontology annotations for CADM4
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IEA
    receptor tyrosine kinase binding GO:0030971 IEA
    vascular endothelial growth factor receptor 1 binding GO:0043183 IEA
    vascular endothelial growth factor receptor 2 binding GO:0043184 IBA
    vascular endothelial growth factor receptor 2 binding GO:0043184 IPI
Biological Process
    regulation of protein phosphorylation GO:0001932 IMP
    negative regulation of protein phosphorylation GO:0001933 IMP
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IMP
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 ISS
    regulation of Rac protein signal transduction GO:0035020 IBA
    regulation of Rac protein signal transduction GO:0035020 IMP
    regulation of cell population proliferation GO:0042127 IMP
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IMP
    regulation of wound healing GO:0061041 IBA
    regulation of wound healing GO:0061041 IMP
    synaptic membrane adhesion GO:0099560 IEA
    negative regulation of vascular endothelial growth factor signaling pathway GO:1900747 ISS
    regulation of cell motility GO:2000145 IMP
Subcellular Localization
    cell leading edge GO:0031252 IDA
    cell-cell contact zone GO:0044291 IBA
    cell-cell contact zone GO:0044291 IDA
    synaptic membrane GO:0097060 IEA
 Experiment description of studies that identified CADM4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CADM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EPHB6  
Affinity Capture-MS Homo sapiens
2 DENND6A 201627
Affinity Capture-MS Homo sapiens
3 SPPL2B 56928
Affinity Capture-MS Homo sapiens
4 TSPAN16  
Affinity Capture-MS Homo sapiens
5 DPP9 91039
Affinity Capture-MS Homo sapiens
6 NR2F6  
Affinity Capture-MS Homo sapiens
7 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
8 TP53 7157
Negative Genetic Homo sapiens
9 HBG2 3048
Affinity Capture-MS Homo sapiens
10 CD69  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HLA-G 3135
Affinity Capture-MS Homo sapiens
12 SCPEP1 59342
Affinity Capture-MS Homo sapiens
13 TNFSF8  
Affinity Capture-MS Homo sapiens
14 ASIC4  
Affinity Capture-MS Homo sapiens
15 CYP2S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DYNC1I1  
Two-hybrid Homo sapiens
17 DIP2A 23181
Affinity Capture-MS Homo sapiens
18 CLEC4A  
Affinity Capture-MS Homo sapiens
19 BMPR1A 657
Affinity Capture-MS Homo sapiens
20 GPAM 57678
Affinity Capture-MS Homo sapiens
21 CEACAM21  
Affinity Capture-MS Homo sapiens
22 SHMT2 6472
Affinity Capture-RNA Homo sapiens
23 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
24 MTRF1  
Affinity Capture-MS Homo sapiens
25 HEPACAM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SFTPC  
Affinity Capture-MS Homo sapiens
27 CERS2 29956
Affinity Capture-MS Homo sapiens
28 ACTA1 58
Affinity Capture-MS Homo sapiens
29 BRICD5  
Affinity Capture-MS Homo sapiens
30 FBXO2 26232
Affinity Capture-MS Homo sapiens
31 NCEH1 57552
Affinity Capture-MS Homo sapiens
32 NMT2 9397
Affinity Capture-MS Homo sapiens
33 EPHA2 1969
Proximity Label-MS Homo sapiens
34 CLEC2B  
Affinity Capture-MS Homo sapiens
35 CSNK1E 1454
Two-hybrid Homo sapiens
36 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
37 MPP7 143098
Affinity Capture-MS Homo sapiens
38 PTCH1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CADM4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here