Gene description for DIP2A
Gene name DIP2 disco-interacting protein 2 homolog A (Drosophila)
Gene symbol DIP2A
Other names/aliases C21orf106
DIP2
Species Homo sapiens
 Database cross references - DIP2A
ExoCarta ExoCarta_23181
Vesiclepedia VP_23181
Entrez Gene 23181
HGNC 17217
MIM 607711
UniProt Q14689  
 DIP2A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for DIP2A
Molecular Function
    acetate-CoA ligase activity GO:0003987 IEA
    protein binding GO:0005515 IPI
Biological Process
    acetyl-CoA biosynthetic process GO:0006085 ISS
    negative regulation of gene expression GO:0010629 IDA
    dendritic spine morphogenesis GO:0060997 ISS
    positive regulation of peptidyl-lysine acetylation GO:2000758 ISS
Subcellular Localization
    mitochondrion GO:0005739 ISS
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    membrane GO:0016020 IDA
    dendritic spine GO:0043197 ISS
 Experiment description of studies that identified DIP2A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DIP2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 STAC3  
Two-hybrid Homo sapiens
3 TRIP13 9319
Two-hybrid Homo sapiens
4 MCAM 4162
Proximity Label-MS Homo sapiens
5 OC90  
Affinity Capture-MS Homo sapiens
6 CX3CL1  
Affinity Capture-MS Homo sapiens
7 EFNB1 1947
Affinity Capture-MS Homo sapiens
8 TGFBR2 7048
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 KCNE3  
Affinity Capture-MS Homo sapiens
11 PSME3 10197
Two-hybrid Homo sapiens
12 AES 166
Two-hybrid Homo sapiens
13 C1orf186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CADM4 199731
Affinity Capture-MS Homo sapiens
15 PDCD1  
Affinity Capture-MS Homo sapiens
16 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LCK 3932
Proximity Label-MS Homo sapiens
19 SPINT2 10653
Affinity Capture-MS Homo sapiens
20 MCC 4163
Affinity Capture-MS Homo sapiens
21 EMD 2010
Proximity Label-MS Homo sapiens
22 EGFR 1956
Affinity Capture-MS Homo sapiens
23 PTPN3  
Two-hybrid Homo sapiens
24 MRGBP  
Affinity Capture-Western Homo sapiens
25 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HCST  
Affinity Capture-MS Homo sapiens
27 IQUB  
Two-hybrid Homo sapiens
28 C11orf52 91894
Proximity Label-MS Homo sapiens
29 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 OPALIN  
Affinity Capture-MS Homo sapiens
31 LPCAT1 79888
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NXF1 10482
Affinity Capture-RNA Homo sapiens
33 PRPS2 5634
Affinity Capture-MS Homo sapiens
34 CEP44  
Two-hybrid Homo sapiens
35 APEX1 328
Affinity Capture-RNA Homo sapiens
36 TRIM52  
Affinity Capture-MS Homo sapiens
37 PPP4R1L  
Affinity Capture-MS Homo sapiens
38 GPATCH2L  
Two-hybrid Homo sapiens
39 TEX264 51368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MBIP  
Two-hybrid Homo sapiens
41 BTN2A1 11120
Affinity Capture-MS Homo sapiens
42 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 FAM46A  
Affinity Capture-MS Homo sapiens
44 IL20RA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DNAJA2 10294
Affinity Capture-MS Homo sapiens
47 SORBS3 10174
Two-hybrid Homo sapiens
48 FAM214B  
Two-hybrid Homo sapiens
49 PTGES3 10728
Affinity Capture-MS Homo sapiens
50 MFN2 9927
Proximity Label-MS Homo sapiens
51 KRAS 3845
Proximity Label-MS Homo sapiens
52 RHOB 388
Proximity Label-MS Homo sapiens
53 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TMCC2  
Two-hybrid Homo sapiens
55 SLC31A1 1317
Affinity Capture-MS Homo sapiens
56 PSME1 5720
Affinity Capture-MS Homo sapiens
57 TRIM39  
Two-hybrid Homo sapiens
58 LMNB2 84823
Affinity Capture-MS Homo sapiens
59 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TTC9C 283237
Affinity Capture-MS Homo sapiens
61 CCDC33 80125
Two-hybrid Homo sapiens
62 ADAMTSL4  
Two-hybrid Homo sapiens
63 JMJD6 23210
Two-hybrid Homo sapiens
64 C3orf52  
Affinity Capture-MS Homo sapiens
65 SELE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ZBTB43  
Two-hybrid Homo sapiens
67 AQP9  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DIP2A is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here