Gene description for LCK
Gene name LCK proto-oncogene, Src family tyrosine kinase
Gene symbol LCK
Other names/aliases IMD22
LSK
YT16
p56lck
pp58lck
Species Homo sapiens
 Database cross references - LCK
ExoCarta ExoCarta_3932
Vesiclepedia VP_3932
Entrez Gene 3932
HGNC 6524
MIM 153390
UniProt P06239  
 LCK identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 34887515    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for LCK
Molecular Function
    CD8 receptor binding GO:0042610 IPI
    protein C-terminus binding GO:0008022 IPI
    protein phosphatase binding GO:0019903 IPI
    identical protein binding GO:0042802 IPI
    protein tyrosine kinase activity GO:0004713 TAS
    protein complex binding GO:0032403 IEA
    phosphatidylinositol 3-kinase binding GO:0043548 IPI
    ATPase binding GO:0051117 IPI
    antigen binding GO:0003823 IEA
    glycoprotein binding GO:0001948 IPI
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 ISS
    protein serine/threonine phosphatase activity GO:0004722 ISS
    protein kinase binding GO:0019901 IPI
    CD4 receptor binding GO:0042609 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    ATP binding GO:0005524 IEA
Biological Process
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
    hemopoiesis GO:0030097 NAS
    leukocyte migration GO:0050900 TAS
    B cell receptor signaling pathway GO:0050853 IBA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 ISS
    positive regulation of T cell receptor signaling pathway GO:0050862 NAS
    blood coagulation GO:0007596 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    platelet activation GO:0030168 TAS
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    response to mechanical stimulus GO:0009612 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of gamma-delta T cell differentiation GO:0045588 IEA
    positive regulation of tyrosine phosphorylation of Stat5 protein GO:0042523 IEA
    dephosphorylation GO:0016311 ISS
    cellular response to peptide hormone stimulus GO:0071375 IBA
    viral process GO:0016032 TAS
    cellular zinc ion homeostasis GO:0006882 ISS
    T cell costimulation GO:0031295 TAS
    T cell differentiation GO:0030217 ISS
    T cell receptor signaling pathway GO:0050852 TAS
    regulation of lymphocyte activation GO:0051249 NAS
    response to zinc ion GO:0010043 IEA
    response to drug GO:0042493 ISS
    positive regulation of gene expression GO:0010628 IEA
    aging GO:0007568 IEA
    regulation of defense response to virus by virus GO:0050690 TAS
    response to hydrogen peroxide GO:0042542 IEA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    innate immune response GO:0045087 TAS
    positive regulation of uterine smooth muscle contraction GO:0070474 IEA
    positive regulation of T cell activation GO:0050870 ISS
    regulation of cell proliferation GO:0042127 IBA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    protein phosphorylation GO:0006468 IDA
Subcellular Localization
    membrane raft GO:0045121 ISS
    endocytic vesicle GO:0030139 IEA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    immunological synapse GO:0001772 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IEA
    pericentriolar material GO:0000242 ISS
 Experiment description of studies that identified LCK in exosomes
1
Experiment ID 79
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
5
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LCK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIK3R1 5295
Reconstituted Complex Homo sapiens
2 CD38 952
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 ARHGAP17 55114
Reconstituted Complex Homo sapiens
4 AXL 558
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
5 DOK2 9046
Affinity Capture-MS Homo sapiens
6 PXN  
Affinity Capture-Western Homo sapiens
7 CD55 1604
Affinity Capture-MS Homo sapiens
8 CD48 962
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 PTPRC 5788
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 PTPN6 5777
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
11 TRAT1 50852
Reconstituted Complex Homo sapiens
12 FAS 355
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
13 CD4 920
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 PTPN11 5781
Affinity Capture-MS Homo sapiens
15 DLG1 1739
Invitro Homo sapiens
Invivo Homo sapiens
16 KIT 3815
Affinity Capture-Western Homo sapiens
17 FASLG 356
Invivo Homo sapiens
18 LCK 3932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MS4A1 931
Affinity Capture-Western Homo sapiens
20 UBE3A 7337
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
21 SQSTM1 8878
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
22 NR2F2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 TUB  
Invivo Homo sapiens
Invitro Homo sapiens
24 LNK  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
25 ADAM15 8751
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
26 PI4KA 5297
Reconstituted Complex Homo sapiens
27 UNC119 9094
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 CBL 867
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
29 SIT1 27240
Invivo Homo sapiens
30 JAK3 3718
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
31 CDC25C  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRKCQ 5588
Two-hybrid Homo sapiens
33 NOTCH1 4851
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 FCGR3A  
Affinity Capture-MS Homo sapiens
35 PAG1 55824
Reconstituted Complex Homo sapiens
36 CTLA4  
Invivo Homo sapiens
Invitro Homo sapiens
37 NEDD9 4739
Affinity Capture-Western Homo sapiens
38 PTK2B 2185
Affinity Capture-Western Homo sapiens
39 ZAP70 7535
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
40 PIK3CA 5290
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 CD2 914
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
42 SKAP1 8631
Reconstituted Complex Homo sapiens
43 RAF1 5894
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
44 IL2RB  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
45 STAT3 6774
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
46 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 KHDRBS1 10657
Invivo Homo sapiens
48 SYK 6850
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
49 TRPV4 59341
Invitro Homo sapiens
Invivo Homo sapiens
50 BCAR1 9564
Reconstituted Complex Homo sapiens
51 Cd3e  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
52 THY1 7070
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
53 PECAM1 5175
Invitro Homo sapiens
54 CTNND1 1500
Biochemical Activity Homo sapiens
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