Gene description for CD247
Gene name CD247 molecule
Gene symbol CD247
Other names/aliases CD3-ZETA
CD3H
CD3Q
CD3Z
IMD25
T3Z
TCRZ
Species Homo sapiens
 Database cross references - CD247
ExoCarta ExoCarta_919
Entrez Gene 919
HGNC 1677
MIM 186780
UniProt P20963  
 CD247 identified in exosomes derived from the following tissue/cell type
Bone marrow cells 15908444    
T cells 11907077    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for CD247
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IEA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 NAS
Biological Process
    regulation of defense response to virus by virus GO:0050690 TAS
    viral process GO:0016032 TAS
    innate immune response GO:0045087 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    T cell costimulation GO:0031295 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    regulation of immune response GO:0050776 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    T cell receptor complex GO:0042101 IDA
    plasma membrane GO:0005886 TAS
    alpha-beta T cell receptor complex GO:0042105 IEA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified CD247 in exosomes
1
Experiment ID 42
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 17
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
E+ cells
MART-1+ T cell
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 18
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD247
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLA2 84174
Invivo Homo sapiens
2 PTPRC 5788
Reconstituted Complex Homo sapiens
3 TFRC 7037
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 SHC1 6464
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 STAT5B 6777
Invivo Homo sapiens
6 CD3D 915
Invivo Homo sapiens
Invitro Homo sapiens
7 STAT5A 6776
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRA@  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
9 SLA  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRAT1 50852
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 UNC119  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
12 GAB2 9846
Affinity Capture-Western Homo sapiens
13 CD247 919
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 JAK3 3718
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 CD5 921
Invivo Homo sapiens
Invitro Homo sapiens
16 FYN 2534
Affinity Capture-MS Homo sapiens
17 NCR1  
Affinity Capture-Western Homo sapiens
18 ZAP70 7535
Affinity Capture-MS Homo sapiens
19 LNK  
Affinity Capture-MS Homo sapiens
20 CD2 914
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
21 DOCK2 1794
Invivo Homo sapiens
22 CD3E 916
Invivo Homo sapiens
23 ATXN2  
Affinity Capture-Western Homo sapiens
24 TIRAP  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CD247 is involved
No pathways found





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