Gene description for DOCK2
Gene name dedicator of cytokinesis 2
Gene symbol DOCK2
Other names/aliases -
Species Homo sapiens
 Database cross references - DOCK2
ExoCarta ExoCarta_1794
Vesiclepedia VP_1794
Entrez Gene 1794
HGNC 2988
MIM 603122
UniProt Q92608  
 DOCK2 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for DOCK2
Molecular Function
    T cell receptor binding GO:0042608 IDA
    protein binding GO:0005515 IPI
    Rac guanyl-nucleotide exchange factor activity GO:0030676 IDA
Biological Process
    negative thymic T cell selection GO:0045060 IEA
    alpha-beta T cell proliferation GO:0046633 IEA
    positive thymic T cell selection GO:0045059 IEA
    viral process GO:0016032 TAS
    regulation of defense response to virus by virus GO:0050690 TAS
    membrane raft polarization GO:0001766 IEA
    actin cytoskeleton organization GO:0030036 TAS
    positive regulation of GTPase activity GO:0043547 IEA
    immunological synapse formation GO:0001771 IEA
    chemotaxis GO:0006935 IEA
    establishment of T cell polarity GO:0001768 IEA
    positive regulation of phagocytosis GO:0050766 ISS
    myeloid dendritic cell activation involved in immune response GO:0002277 ISS
    small GTPase mediated signal transduction GO:0007264 IEA
    macropinocytosis GO:0044351 ISS
Subcellular Localization
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    endomembrane system GO:0012505 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified DOCK2 in exosomes
1
Experiment ID 79
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOCK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRKL 1399
Invivo Homo sapiens
Invitro Homo sapiens
2 RAC1 5879
Reconstituted Complex Homo sapiens
3 CD247 919
Invivo Homo sapiens
4 VAV1 7409
Invitro Homo sapiens
5 RAC2 5880
Reconstituted Complex Homo sapiens
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