Gene description for ELMO1
Gene name engulfment and cell motility 1
Gene symbol ELMO1
Other names/aliases CED-12
CED12
ELMO-1
Species Homo sapiens
 Database cross references - ELMO1
ExoCarta ExoCarta_9844
Vesiclepedia VP_9844
Entrez Gene 9844
HGNC 16286
MIM 606420
UniProt Q92556  
 ELMO1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for ELMO1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IPI
Biological Process
    phagocytosis, engulfment GO:0006911 IGI
    apoptotic process GO:0006915 NAS
    actin filament organization GO:0007015 IBA
    Rac protein signal transduction GO:0016601 IGI
    actin cytoskeleton organization GO:0030036 IGI
    cell motility GO:0048870 IBA
    cell motility GO:0048870 IGI
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    guanyl-nucleotide exchange factor complex GO:0032045 IBA
    guanyl-nucleotide exchange factor complex GO:0032045 IDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ELMO1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ELMO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
3 CALML3 810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DOCK1 1793
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DOCK2 1794
Two-hybrid Homo sapiens
6 USP4 7375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DOCK4 9732
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CRK 1398
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 RHOG 391
Two-hybrid Homo sapiens
11 ZNF398  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 APBB1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
13 NUAK1  
Affinity Capture-MS Homo sapiens
14 ARHGEF16 27237
Affinity Capture-Western Homo sapiens
15 ELMO2 63916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 POMGNT2  
Affinity Capture-MS Homo sapiens
17 TRIM54  
Two-hybrid Homo sapiens
18 ARL4A  
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
19 HCK 3055
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 TTC19  
Affinity Capture-MS Homo sapiens
21 PTGS2 5743
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 NTRK1 4914
Affinity Capture-MS Homo sapiens
23 CRKL 1399
Affinity Capture-MS Homo sapiens
24 GRIN1 2902
Affinity Capture-MS Homo sapiens
25 ELMO3 79767
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ADGRB3 577
Two-hybrid Homo sapiens
28 CEBPA  
Protein-peptide Homo sapiens
29 DOCK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CDC27 996
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 BAI1  
Two-hybrid Homo sapiens
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