Gene description for CDC27
Gene name cell division cycle 27
Gene symbol CDC27
Other names/aliases ANAPC3
APC3
CDC27Hs
D0S1430E
D17S978E
H-NUC
HNUC
NUC2
Species Homo sapiens
 Database cross references - CDC27
ExoCarta ExoCarta_996
Vesiclepedia VP_996
Entrez Gene 996
HGNC 1728
MIM 116946
UniProt P30260  
 CDC27 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CDC27
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IPI
Biological Process
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IBA
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IMP
    regulation of mitotic cell cycle GO:0007346 NAS
    protein ubiquitination GO:0016567 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IDA
    anaphase-promoting complex-dependent catabolic process GO:0031145 NAS
    cell division GO:0051301 IBA
    regulation of meiotic cell cycle GO:0051445 NAS
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IDA
    protein branched polyubiquitination GO:0141198 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IBA
    anaphase-promoting complex GO:0005680 IDA
    anaphase-promoting complex GO:0005680 IPI
    anaphase-promoting complex GO:0005680 NAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 TAS
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified CDC27 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CDC27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 REV1  
Affinity Capture-Western Homo sapiens
2 MVP 9961
Co-fractionation Homo sapiens
3 GRAP2 9402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 CDC26 246184
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
6 Cdk1 12534
Affinity Capture-MS Mus musculus
7 Anapc13  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
8 CUEDC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 PAXIP1  
Affinity Capture-MS Homo sapiens
10 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
11 POU2F3  
Affinity Capture-MS Homo sapiens
12 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
13 RABEP2 79874
Affinity Capture-MS Homo sapiens
14 EPHX1 2052
Co-fractionation Homo sapiens
15 Nek2  
Affinity Capture-MS Mus musculus
16 ANAPC5 51433
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
17 ANAPC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
18 BUB1B  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 Sirt2 64383
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
20 BRD2  
Affinity Capture-MS Homo sapiens
21 LAMTOR2 28956
Affinity Capture-MS Homo sapiens
22 RSBN1  
Affinity Capture-MS Homo sapiens
23 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
24 TAF5  
Co-fractionation Homo sapiens
25 HSF2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
26 FZR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 CAPZB 832
Affinity Capture-MS Homo sapiens
28 MRFAP1L1  
Affinity Capture-MS Homo sapiens
29 CDC16 8881
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
30 EMILIN1 11117
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
31 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DYRK1A 1859
Affinity Capture-MS Homo sapiens
33 CFAP97  
Affinity Capture-MS Homo sapiens
34 GKN1  
Two-hybrid Homo sapiens
35 Ppp5c  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
36 SPATC1  
Affinity Capture-Western Homo sapiens
37 Bub1b 12236
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
38 MDC1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 MAD2L2 10459
Affinity Capture-Western Homo sapiens
40 HMGA1 3159
Affinity Capture-MS Homo sapiens
41 ANAPC11  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 THRSP  
Affinity Capture-MS Homo sapiens
43 LAMTOR3 8649
Affinity Capture-MS Homo sapiens
44 COMT 1312
Two-hybrid Homo sapiens
45 CDC27 996
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
46 SRP68 6730
Affinity Capture-MS Homo sapiens
47 FBXO5  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 ANAPC7 51434
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
49 ANAPC16  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
50 MAD2L1 4085
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 MAGIX 79917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FOXP3  
Affinity Capture-MS Homo sapiens
53 RBPJ 3516
Affinity Capture-MS Homo sapiens
54 NINL  
Affinity Capture-Western Homo sapiens
55 RB1 5925
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
56 H2AFZ 3015
Affinity Capture-MS Homo sapiens
57 EIF6 3692
Proximity Label-MS Homo sapiens
58 MIB1 57534
Affinity Capture-MS Homo sapiens
59 UBE4B 10277
Affinity Capture-Western Homo sapiens
60 PPP5C 5536
Reconstituted Complex Homo sapiens
61 Anapc5 59008
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
62 CDC23 8697
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
63 USP11 8237
Affinity Capture-MS Homo sapiens
64 C9orf78 51759
Affinity Capture-MS Homo sapiens
65 CDKN1A  
Affinity Capture-Western Homo sapiens
66 ZNF526  
Affinity Capture-MS Homo sapiens
67 RPL5 6125
Co-fractionation Homo sapiens
68 TFDP1  
Affinity Capture-Western Homo sapiens
69 CCNB1 891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 CDH1 999
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 C9orf72  
Affinity Capture-MS Homo sapiens
72 VCP 7415
Affinity Capture-MS Homo sapiens
73 NTRK1 4914
Affinity Capture-MS Homo sapiens
74 Gm9174  
Affinity Capture-MS Mus musculus
75 TTLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 EIF2B2 8892
Co-fractionation Homo sapiens
77 TEAD2  
Affinity Capture-MS Homo sapiens
78 DCTN1 1639
Proximity Label-MS Homo sapiens
79 SMAD2 4087
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
80 E2F1 1869
Affinity Capture-Western Homo sapiens
81 ANAPC10  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
82 NEK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
83 ANAPC4 29945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
84 BRD4 23476
Affinity Capture-MS Homo sapiens
85 SKP2  
Affinity Capture-Western Homo sapiens
86 PPM1G 5496
Co-fractionation Homo sapiens
87 ID2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
88 NUMA1 4926
Affinity Capture-MS Homo sapiens
89 UBE2S  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
90 Cdc27  
Affinity Capture-MS Mus musculus
91 ANAPC13 25847
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
92 ANO6 196527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 CCNA2 890
Affinity Capture-Western Homo sapiens
94 Cdc23  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
95 SMARCAD1  
Affinity Capture-MS Homo sapiens
96 ELMO1 9844
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
97 TP53BP1 7158
Affinity Capture-MS Homo sapiens
98 XPO1 7514
Affinity Capture-MS Homo sapiens
99 EGLN3  
Affinity Capture-MS Homo sapiens
100 Rab5b 19344
Affinity Capture-MS Mus musculus
101 MAD1L1  
Affinity Capture-Western Homo sapiens
102 SMAD3 4088
Affinity Capture-Western Homo sapiens
103 CTNNB1 1499
Reconstituted Complex Homo sapiens
104 MAD2L1BP  
Affinity Capture-Western Homo sapiens
105 KPNB1 3837
Cross-Linking-MS (XL-MS) Homo sapiens
106 DAB2IP 153090
Affinity Capture-Western Homo sapiens
107 PTEN 5728
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
108 ADNP 23394
Two-hybrid Homo sapiens
109 DCPS 28960
Two-hybrid Homo sapiens
110 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 H1F0 3005
Affinity Capture-MS Homo sapiens
112 PYCR2 29920
Affinity Capture-MS Homo sapiens
113 CKS1B  
Reconstituted Complex Homo sapiens
114 PPP2CA 5515
Affinity Capture-Western Homo sapiens
115 Paxip1  
Affinity Capture-MS Mus musculus
116 Anapc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
117 Cdc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
118 AURKB 9212
Affinity Capture-Western Homo sapiens
119 FOXM1  
Affinity Capture-Western Homo sapiens
120 MAPRE3  
Proximity Label-MS Homo sapiens
121 BIRC6 57448
Affinity Capture-Western Homo sapiens
122 CUL9  
Affinity Capture-MS Homo sapiens
123 FBXO43  
Affinity Capture-Western Homo sapiens
124 HECW2  
Affinity Capture-MS Homo sapiens
125 CDCA3 83461
Affinity Capture-MS Homo sapiens
126 FDPS 2224
Affinity Capture-MS Homo sapiens
127 EPN3 55040
Affinity Capture-MS Homo sapiens
128 CSNK2A2 1459
Co-fractionation Homo sapiens
129 Anapc1  
Affinity Capture-MS Mus musculus
130 CHD8 57680
Affinity Capture-MS Homo sapiens
131 RHOB 388
Proximity Label-MS Homo sapiens
132 FOXQ1  
Affinity Capture-MS Homo sapiens
133 PPP2R5A 5525
Affinity Capture-Western Homo sapiens
134 Mad2l1 56150
Affinity Capture-MS Mus musculus
135 CCND1 595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
136 BRD3 8019
Affinity Capture-MS Homo sapiens
137 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 TAF4 6874
Co-fractionation Homo sapiens
139 PABPC4 8761
Affinity Capture-RNA Homo sapiens
Protein-RNA Homo sapiens
140 ATRX 546
Affinity Capture-MS Homo sapiens
141 TACC3  
Affinity Capture-Western Homo sapiens
142 Anapc2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
143 USP37  
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
144 RRM2 6241
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 FOXO3  
Affinity Capture-MS Homo sapiens
146 CDC20 991
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
147 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
148 EIF3C 8663
Co-fractionation Homo sapiens
149 ILF3 3609
Affinity Capture-RNA Homo sapiens
150 TRIM33 51592
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
151 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 PIN1 5300
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
153 SKP1 6500
Affinity Capture-MS Homo sapiens
154 FOXE1  
Affinity Capture-MS Homo sapiens
155 SQSTM1 8878
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
156 PTTG1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
157 ANAPC1 64682
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
158 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
159 TTK 7272
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
160 BUB3 9184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
161 ELMO2 63916
Affinity Capture-Western Homo sapiens
162 Cdc20  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
163 PCNA 5111
Affinity Capture-Western Homo sapiens
164 ANAPC2 29882
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
165 MCPH1  
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
166 PENK 5179
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 NCL 4691
Co-fractionation Homo sapiens
168 PSMA5 5686
Affinity Capture-MS Homo sapiens
169 AURKA 6790
Affinity Capture-Western Homo sapiens
170 PARK2  
Affinity Capture-MS Homo sapiens
171 BRD1 23774
Affinity Capture-MS Homo sapiens
172 SNW1 22938
Affinity Capture-MS Homo sapiens
173 KRAS 3845
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
174 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 NUCKS1 64710
Affinity Capture-MS Homo sapiens
176 PHF8  
Affinity Capture-MS Homo sapiens
177 RPA3 6119
Proximity Label-MS Homo sapiens
178 C16orf87 388272
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 NOTCH1 4851
Affinity Capture-MS Homo sapiens
180 EP300 2033
Affinity Capture-MS Homo sapiens
181 CDC5L 988
Affinity Capture-MS Homo sapiens
182 Cdc26  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which CDC27 is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic exit in cancer due to RB1 defects TAS Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Adaptive Immune System TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC-Cdc20 mediated degradation of Nek2A TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase TAS Reactome
Disease TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Immune System TAS Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex TAS Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components TAS Reactome
M Phase TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Separation of Sister Chromatids TAS Reactome
Switching of origins to a post-replicative state TAS Reactome
Synthesis of DNA TAS Reactome
Transcriptional Regulation by VENTX TAS Reactome





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