Gene description for CDC27
Gene name cell division cycle 27
Gene symbol CDC27
Other names/aliases ANAPC3
APC3
CDC27Hs
D0S1430E
D17S978E
H-NUC
HNUC
NUC2
Species Homo sapiens
 Database cross references - CDC27
ExoCarta ExoCarta_996
Entrez Gene 996
HGNC 1728
MIM 116946
UniProt P30260  
 CDC27 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for CDC27
Molecular Function
    protein phosphatase binding GO:0019903 IPI
    protein binding GO:0005515 IPI
Biological Process
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IMP
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    mitotic spindle assembly checkpoint GO:0007094 TAS
    cell proliferation GO:0008283 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    protein K11-linked ubiquitination GO:0070979 IDA
    mitotic cell cycle GO:0000278 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
Subcellular Localization
    centrosome GO:0005813 IDA
    spindle microtubule GO:0005876 IDA
    spindle GO:0005819 TAS
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    anaphase-promoting complex GO:0005680 IDA
 Experiment description of studies that identified CDC27 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC20  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 ANAPC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 PIN1 5300
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
4 APC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 SPATC1  
Affinity Capture-Western Homo sapiens
6 ANAPC5 51433
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 ANAPC10  
Affinity Capture-Western Homo sapiens
8 FZR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CDC23 8697
Affinity Capture-Western Homo sapiens
10 PPP5C 5536
Reconstituted Complex Homo sapiens
11 BUB1B  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 CDH1 999
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 MAD2L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 SMAD3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 SMAD2 4087
Affinity Capture-Western Homo sapiens
16 ANAPC11  
Affinity Capture-Western Homo sapiens
17 HOXC10  
Affinity Capture-MS Homo sapiens
18 ANAPC7 51434
Affinity Capture-Western Homo sapiens
19 CDC16 8881
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 ANAPC1 64682
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
21 Ppp5c  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here