Experiment description of studies that identified HIST2H2BE in exosomes |
1 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
2 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
3 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
4 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
5 |
Experiment ID |
226 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✔
CD9|FLOT1
|
EV Membrane markers |
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
PubMed ID |
26027894
|
Organism |
Homo sapiens |
Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
Journal name |
Journal of Extracellular Vesicles
|
Publication year |
2015 |
Sample |
Endothelial cells |
Sample name |
HMEC-1 |
Isolation/purification methods |
Differential ultracentrifugation Sucrose density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA |
Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting |
|
|
6 |
Experiment ID |
126 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
7 |
Experiment ID |
224 |
ISEV standards |
✔
EM|AFM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✔
GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
8 |
Experiment ID |
211 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
cytochrome c|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
IGROV1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
9 |
Experiment ID |
212 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
EpCAM|TFRC
|
EV Membrane markers |
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23333927
|
Organism |
Homo sapiens |
Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
Journal name |
J Proteomics
|
Publication year |
2013 |
Sample |
Ovarian cancer cells |
Sample name |
OVCAR-3 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.09-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
138 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
11 |
Experiment ID |
139 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
12 |
Experiment ID |
140 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
13 |
Experiment ID |
141 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
14 |
Experiment ID |
142 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
15 |
Experiment ID |
143 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
LNCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
16 |
Experiment ID |
145 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
CD10
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
C4-2 - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
17 |
Experiment ID |
146 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
RWPE - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
18 |
Experiment ID |
275 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix|RAB5A
|
EV Cytosolic markers |
✔
CD9|CD82|CD63|CD81
|
EV Membrane markers |
✔
AIF
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25844599
|
Organism |
Homo sapiens |
Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Docetaxel sensitive |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.12-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry/Flow cytometry/Western blotting |
|
|
19 |
Experiment ID |
274 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix|RAB5A
|
EV Cytosolic markers |
✔
CD9|CD82|CD63|CD81
|
EV Membrane markers |
✔
AIF
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25844599
|
Organism |
Homo sapiens |
Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Prostate cancer cells |
Sample name |
DU145 - Docetaxel resistant |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.18 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Flow cytometry Western blotting |
|
|
20 |
Experiment ID |
66 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
21 |
Experiment ID |
191 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD81|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|