Gene description for INCENP
Gene name inner centromere protein antigens 135/155kDa
Gene symbol INCENP
Other names/aliases -
Species Homo sapiens
 Database cross references - INCENP
ExoCarta ExoCarta_3619
Vesiclepedia VP_3619
Entrez Gene 3619
HGNC 6058
MIM 604411
UniProt Q9NQS7  
 INCENP identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for INCENP
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    cytokinesis GO:0000910 IMP
    mitotic nuclear division GO:0007067 IEA
    chromosome segregation GO:0007059 IMP
Subcellular Localization
    pericentric heterochromatin GO:0005721 IDA
    cytosol GO:0005829 TAS
    protein complex GO:0043234 IDA
    kinetochore GO:0000776 IDA
    midbody GO:0030496 IEA
    condensed chromosome kinetochore GO:0000777 IEA
    central element GO:0000801 IEA
    spindle GO:0005819 IDA
    chromocenter GO:0010369 IEA
    lateral element GO:0000800 IEA
    chromosome, centromeric region GO:0000775 IDA
    microtubule GO:0005874 IEA
 Experiment description of studies that identified INCENP in exosomes
1
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for INCENP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX5 23468
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 H2AFZ 3015
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 BIRC5  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
4 CDCA8 55143
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 AURKB 9212
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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