Gene description for EIF2B5
Gene name eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Gene symbol EIF2B5
Other names/aliases CACH
CLE
EIF-2B
EIF2Bepsilon
LVWM
Species Homo sapiens
 Database cross references - EIF2B5
ExoCarta ExoCarta_8893
Vesiclepedia VP_8893
Entrez Gene 8893
HGNC 3261
MIM 603945
UniProt Q13144  
 EIF2B5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for EIF2B5
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 NAS
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 IBA
    translation initiation factor binding GO:0031369 ISS
Biological Process
    ovarian follicle development GO:0001541 IMP
    cytoplasmic translational initiation GO:0002183 IDA
    translational initiation GO:0006413 IDA
    response to heat GO:0009408 IMP
    response to heat GO:0009408 ISS
    response to heat GO:0009408 TAS
    response to glucose GO:0009749 ISS
    astrocyte development GO:0014002 IMP
    oligodendrocyte development GO:0014003 IMP
    hippocampus development GO:0021766 IEA
    response to endoplasmic reticulum stress GO:0034976 IMP
    myelination GO:0042552 IMP
    positive regulation of apoptotic process GO:0043065 IEA
    response to peptide hormone GO:0043434 ISS
    positive regulation of translational initiation GO:0045948 ISS
    astrocyte differentiation GO:0048708 IMP
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2B complex GO:0005851 IBA
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
 Experiment description of studies that identified EIF2B5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Two-hybrid Homo sapiens
2 TACR3  
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 EIF4G3 8672
Co-fractionation Homo sapiens
5 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CD68 968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 EIF2S3 1968
Affinity Capture-MS Homo sapiens
8 EIF3K 27335
Co-fractionation Homo sapiens
9 EIF2B2 8892
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 JPH1 56704
Affinity Capture-MS Homo sapiens
11 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 CSE1L 1434
Cross-Linking-MS (XL-MS) Homo sapiens
14 MYO5C 55930
Affinity Capture-MS Homo sapiens
15 CAMSAP2  
Affinity Capture-MS Homo sapiens
16 INCENP 3619
Affinity Capture-MS Homo sapiens
17 FFAR1  
Affinity Capture-MS Homo sapiens
18 PNKD  
Affinity Capture-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 KIF20A 10112
Affinity Capture-MS Homo sapiens
22 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GLG1 2734
Affinity Capture-MS Homo sapiens
24 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 FAM174A 345757
Affinity Capture-MS Homo sapiens
26 FBXW7  
Affinity Capture-MS Homo sapiens
27 STX8 9482
Affinity Capture-MS Homo sapiens
28 CLEC2B  
Affinity Capture-MS Homo sapiens
29 DDRGK1 65992
Affinity Capture-MS Homo sapiens
30 SYT2  
Affinity Capture-MS Homo sapiens
31 FAM210A  
Affinity Capture-MS Homo sapiens
32 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TACSTD2 4070
Affinity Capture-MS Homo sapiens
34 TMEM192 201931
Affinity Capture-MS Homo sapiens
35 ATG16L1 55054
Affinity Capture-MS Homo sapiens
36 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 GPR17 2840
Affinity Capture-MS Homo sapiens
40 GOLIM4 27333
Affinity Capture-MS Homo sapiens
41 GMPPA 29926
Affinity Capture-MS Homo sapiens
42 ATAD3B 83858
Affinity Capture-MS Homo sapiens
43 CD70 970
Affinity Capture-MS Homo sapiens
44 POF1B 79983
Affinity Capture-MS Homo sapiens
45 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CD74 972
Affinity Capture-MS Homo sapiens
47 EXOG 9941
Affinity Capture-MS Homo sapiens
48 PARK2  
Affinity Capture-MS Homo sapiens
49 TMEM55B 90809
Affinity Capture-MS Homo sapiens
50 PLD1 5337
Affinity Capture-MS Homo sapiens
51 ILVBL 10994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MCM10 55388
Affinity Capture-MS Homo sapiens
53 YME1L1 10730
Affinity Capture-MS Homo sapiens
54 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 TRPM7 54822
Affinity Capture-MS Homo sapiens
57 PTAFR  
Affinity Capture-MS Homo sapiens
58 LETMD1  
Affinity Capture-MS Homo sapiens
59 HECTD1 25831
Affinity Capture-MS Homo sapiens
60 MICU1 10367
Affinity Capture-MS Homo sapiens
61 EIF2B4 8890
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
62 CACNG4  
Affinity Capture-MS Homo sapiens
63 P2RX4 5025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CD40 958
Affinity Capture-MS Homo sapiens
65 SGPL1 8879
Affinity Capture-MS Homo sapiens
66 GMPPB 29925
Affinity Capture-MS Homo sapiens
67 IVL 3713
Affinity Capture-MS Homo sapiens
68 PHLDB3  
Affinity Capture-MS Homo sapiens
69 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 FGF14  
Affinity Capture-MS Homo sapiens
71 BTNL9  
Affinity Capture-MS Homo sapiens
72 XPO1 7514
Affinity Capture-MS Homo sapiens
73 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 TVP23C  
Affinity Capture-MS Homo sapiens
75 SLC27A4 10999
Affinity Capture-MS Homo sapiens
76 IQCB1  
Affinity Capture-MS Homo sapiens
77 ASPM 259266
Affinity Capture-MS Homo sapiens
78 CUL3 8452
Affinity Capture-MS Homo sapiens
79 TTC3 7267
Affinity Capture-MS Homo sapiens
80 PEX10  
Affinity Capture-MS Homo sapiens
81 CLEC4A  
Affinity Capture-MS Homo sapiens
82 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MYO5A 4644
Affinity Capture-MS Homo sapiens
84 EIF2B3 8891
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
85 CD226 10666
Affinity Capture-MS Homo sapiens
86 MAP7D1 55700
Affinity Capture-MS Homo sapiens
87 SMDT1  
Affinity Capture-MS Homo sapiens
88 C3orf18  
Affinity Capture-MS Homo sapiens
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 GPR45  
Affinity Capture-MS Homo sapiens
91 LGALS7B 653499
Affinity Capture-MS Homo sapiens
92 MYO9B 4650
Affinity Capture-MS Homo sapiens
93 KCNN4 3783
Affinity Capture-MS Homo sapiens
94 PVRL4  
Affinity Capture-MS Homo sapiens
95 ENTPD2 954
Affinity Capture-MS Homo sapiens
96 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 TMEM55A 55529
Affinity Capture-MS Homo sapiens
98 ASH1L 55870
Affinity Capture-MS Homo sapiens
99 ABCB10 23456
Affinity Capture-MS Homo sapiens
100 EFNB2 1948
Affinity Capture-MS Homo sapiens
101 OSBPL8 114882
Affinity Capture-MS Homo sapiens
102 MAP7D2 256714
Affinity Capture-MS Homo sapiens
103 STX11 8676
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
104 PIN4 5303
Affinity Capture-MS Homo sapiens
105 RPS16 6217
Affinity Capture-MS Homo sapiens
106 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
107 EIF3I 8668
Co-fractionation Homo sapiens
108 PCDH9  
Affinity Capture-MS Homo sapiens
109 B3GALT6 126792
Affinity Capture-MS Homo sapiens
110 BTC  
Affinity Capture-MS Homo sapiens
111 ZNF445  
Affinity Capture-MS Homo sapiens
112 TNFSF14  
Affinity Capture-MS Homo sapiens
113 MEN1 4221
Affinity Capture-MS Homo sapiens
114 EFNB1 1947
Affinity Capture-MS Homo sapiens
115 OPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 S100A9 6280
Affinity Capture-MS Homo sapiens
117 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 SPN 6693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 SEC62 7095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 MGME1  
Affinity Capture-MS Homo sapiens
121 CEP78 84131
Affinity Capture-MS Homo sapiens
122 TPST2 8459
Affinity Capture-MS Homo sapiens
123 KRAS 3845
Negative Genetic Homo sapiens
124 MIS18BP1  
Affinity Capture-MS Homo sapiens
125 CHSY1 22856
Affinity Capture-MS Homo sapiens
126 ZNF865  
Affinity Capture-MS Homo sapiens
127 ARSF 416
Affinity Capture-MS Homo sapiens
128 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 FAM210B  
Affinity Capture-MS Homo sapiens
130 S1PR4  
Affinity Capture-MS Homo sapiens
131 EIF3L 51386
Co-fractionation Homo sapiens
View the network image/svg+xml



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