Gene description for EIF2B5
Gene name eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Gene symbol EIF2B5
Other names/aliases CACH
CLE
EIF-2B
EIF2Bepsilon
LVWM
Species Homo sapiens
 Database cross references - EIF2B5
ExoCarta ExoCarta_8893
Entrez Gene 8893
HGNC 3261
MIM 603945
UniProt Q13144  
 EIF2B5 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for EIF2B5
Molecular Function
    protein binding GO:0005515 IPI
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    translation initiation factor binding GO:0031369 ISS
    translation initiation factor activity GO:0003743 NAS
Biological Process
    oligodendrocyte development GO:0014003 IMP
    translation GO:0006412 TAS
    astrocyte development GO:0014002 IMP
    astrocyte differentiation GO:0048708 IMP
    negative regulation of translational initiation in response to stress GO:0032057 ISS
    translational initiation GO:0006413 TAS
    response to heat GO:0009408 TAS
    response to endoplasmic reticulum stress GO:0034976 IMP
    positive regulation of GTPase activity GO:0043547 ISS
    response to peptide hormone GO:0043434 ISS
    response to glucose GO:0009749 ISS
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of translational initiation GO:0045948 ISS
    myelination GO:0042552 IMP
    gene expression GO:0010467 TAS
    ovarian follicle development GO:0001541 IMP
    cellular response to drug GO:0035690 IDA
Subcellular Localization
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 ISS
 Experiment description of studies that identified EIF2B5 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF2B4 8890
Reconstituted Complex Homo sapiens
2 EIF2B3 8891
Reconstituted Complex Homo sapiens
3 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 EIF2B2 8892
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which EIF2B5 is involved
PathwayEvidenceSource
Recycling of eIF2:GDP TAS Reactome





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