Gene description for SGPL1
Gene name sphingosine-1-phosphate lyase 1
Gene symbol SGPL1
Other names/aliases S1PL
SPL
Species Homo sapiens
 Database cross references - SGPL1
ExoCarta ExoCarta_8879
Vesiclepedia VP_8879
Entrez Gene 8879
HGNC 10817
MIM 603729
UniProt O95470  
 SGPL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SGPL1
Molecular Function
    protein binding GO:0005515 IPI
    sphinganine-1-phosphate aldolase activity GO:0008117 IBA
    sphinganine-1-phosphate aldolase activity GO:0008117 IDA
    sphinganine-1-phosphate aldolase activity GO:0008117 IMP
    sphinganine-1-phosphate aldolase activity GO:0008117 NAS
    sphinganine-1-phosphate aldolase activity GO:0008117 TAS
    pyridoxal phosphate binding GO:0030170 IEA
Biological Process
    luteinization GO:0001553 IEA
    vasculogenesis GO:0001570 IEA
    kidney development GO:0001822 IEA
    fatty acid metabolic process GO:0006631 IDA
    ceramide metabolic process GO:0006672 IDA
    spermatogenesis GO:0007283 IEA
    androgen metabolic process GO:0008209 IEA
    estrogen metabolic process GO:0008210 IEA
    post-embryonic development GO:0009791 IEA
    fibroblast migration GO:0010761 IEA
    hemopoiesis GO:0030097 IEA
    sphingolipid catabolic process GO:0030149 IBA
    sphingolipid catabolic process GO:0030149 IDA
    sphingolipid catabolic process GO:0030149 IMP
    sphingolipid catabolic process GO:0030149 NAS
    sphingolipid catabolic process GO:0030149 TAS
    Leydig cell differentiation GO:0033327 IEA
    regulation of multicellular organism growth GO:0040014 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    skeletal system morphogenesis GO:0048705 IEA
    roof of mouth development GO:0060021 IEA
    face morphogenesis GO:0060325 IEA
    apoptotic signaling pathway GO:0097190 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified SGPL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SGPL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 HBD 3045
Affinity Capture-MS Homo sapiens
4 CACNG5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 STATH  
Two-hybrid Homo sapiens
6 FA2H  
Two-hybrid Homo sapiens
7 TUSC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RPL10 6134
Affinity Capture-MS Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 STX4 6810
Two-hybrid Homo sapiens
12 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
13 PAQR5  
Two-hybrid Homo sapiens
14 ATP5L 10632
Co-fractionation Homo sapiens
15 CAPZB 832
Affinity Capture-MS Homo sapiens
16 PARK2  
Affinity Capture-MS Homo sapiens
17 HEATR3 55027
Affinity Capture-MS Homo sapiens
18 BTN2A1 11120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RBM39 9584
Affinity Capture-MS Homo sapiens
20 CHODL 140578
Affinity Capture-MS Homo sapiens
21 FBXW7  
Affinity Capture-MS Homo sapiens
22 TM9SF4 9777
Co-fractionation Homo sapiens
23 STX8 9482
Two-hybrid Homo sapiens
24 NPM1 4869
Affinity Capture-MS Homo sapiens
25 PPP6R1 22870
Affinity Capture-MS Homo sapiens
26 GPBAR1  
Affinity Capture-MS Homo sapiens
27 PIGH  
Affinity Capture-MS Homo sapiens
28 ATP5O 539
Co-fractionation Homo sapiens
29 C3orf52  
Affinity Capture-MS Homo sapiens
30 TMEM97 27346
Two-hybrid Homo sapiens
31 DGCR2 9993
Affinity Capture-MS Homo sapiens
32 CCDC47 57003
Affinity Capture-MS Homo sapiens
33 ATG16L1 55054
Affinity Capture-MS Homo sapiens
34 TOMM40 10452
Co-fractionation Homo sapiens
35 IL17RA 23765
Affinity Capture-MS Homo sapiens
36 LGALS9 3965
Affinity Capture-MS Homo sapiens
37 MMD2  
Two-hybrid Homo sapiens
38 PKN2 5586
Affinity Capture-MS Homo sapiens
39 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CSNK2B 1460
Affinity Capture-MS Homo sapiens
41 UQCRQ 27089
Co-fractionation Homo sapiens
42 FAM20C 56975
Affinity Capture-MS Homo sapiens
43 MARCH5  
Two-hybrid Homo sapiens
44 ANKRD12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ITLN1 55600
Affinity Capture-MS Homo sapiens
46 NAP1L2  
Affinity Capture-MS Homo sapiens
47 BSG 682
Affinity Capture-MS Homo sapiens
48 AURKA 6790
Affinity Capture-MS Homo sapiens
49 CD70 970
Affinity Capture-MS Homo sapiens
50 MTCH2 23788
Co-fractionation Homo sapiens
51 EFNA5  
Two-hybrid Homo sapiens
52 IFITM3 10410
Two-hybrid Homo sapiens
53 TSPAN4 7106
Two-hybrid Homo sapiens
54 CCL4L2  
Two-hybrid Homo sapiens
55 EIF2B5 8893
Affinity Capture-MS Homo sapiens
56 MFSD6 54842
Two-hybrid Homo sapiens
57 VSIG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ESRRB  
Affinity Capture-MS Homo sapiens
59 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 SAR1B 51128
Affinity Capture-MS Homo sapiens
62 HLA-C 3107
Affinity Capture-MS Homo sapiens
63 CCDC107  
Affinity Capture-MS Homo sapiens
64 FBXL6  
Affinity Capture-MS Homo sapiens
65 CLSTN2 64084
Affinity Capture-MS Homo sapiens
66 CYC1 1537
Co-fractionation Homo sapiens
67 TNFRSF10C  
Affinity Capture-MS Homo sapiens
68 SLC30A3  
Two-hybrid Homo sapiens
69 TECR 9524
Co-fractionation Homo sapiens
70 CLDN19  
Two-hybrid Homo sapiens
71 VDAC2 7417
Co-fractionation Homo sapiens
72 LRRTM4  
Affinity Capture-MS Homo sapiens
73 SCAMP5  
Two-hybrid Homo sapiens
74 CD6 923
Affinity Capture-MS Homo sapiens
75 BCL2L2 599
Two-hybrid Homo sapiens
76 CYB5B 80777
Affinity Capture-MS Homo sapiens
77 APLNR  
Affinity Capture-MS Homo sapiens
78 CXCR4 7852
Affinity Capture-MS Homo sapiens
79 TTYH1  
Affinity Capture-MS Homo sapiens
80 AIFM1 9131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 CANX 821
Affinity Capture-MS Homo sapiens
82 C14orf1 11161
Two-hybrid Homo sapiens
83 LRRC59 55379
Affinity Capture-MS Homo sapiens
84 RTP2  
Two-hybrid Homo sapiens
85 HMOX2 3163
Two-hybrid Homo sapiens
86 IQCB1  
Affinity Capture-MS Homo sapiens
87 FAM177A1 283635
Two-hybrid Homo sapiens
88 GPR182  
Affinity Capture-MS Homo sapiens
89 BRD8 10902
Affinity Capture-MS Homo sapiens
90 ATP5F1 515
Co-fractionation Homo sapiens
91 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 AQP3  
Two-hybrid Homo sapiens
93 MORF4L1  
Affinity Capture-MS Homo sapiens
94 TMEM242  
Two-hybrid Homo sapiens
95 TMEM173  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
96 SAAL1 113174
Affinity Capture-MS Homo sapiens
97 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
98 C3orf18  
Affinity Capture-MS Homo sapiens
99 RPA3 6119
Proximity Label-MS Homo sapiens
100 ATP2A1 487
Proximity Label-MS Homo sapiens
101 GPR45  
Affinity Capture-MS Homo sapiens
102 ARRDC3 57561
Affinity Capture-MS Homo sapiens
103 PPAPDC1A  
Two-hybrid Homo sapiens
104 DDB1 1642
Affinity Capture-MS Homo sapiens
105 DDRGK1 65992
Affinity Capture-MS Homo sapiens
106 PLP2 5355
Two-hybrid Homo sapiens
107 CRELD1 78987
Affinity Capture-MS Homo sapiens
108 VDAC1 7416
Co-fractionation Homo sapiens
109 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 NEUROG3  
Affinity Capture-MS Homo sapiens
111 RPS16 6217
Affinity Capture-MS Homo sapiens
112 AQP9  
Two-hybrid Homo sapiens
113 IMMT 10989
Co-fractionation Homo sapiens
114 MBOAT7 79143
Affinity Capture-MS Homo sapiens
115 UNC50  
Two-hybrid Homo sapiens
116 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
117 FASN 2194
Negative Genetic Homo sapiens
118 MIP  
Two-hybrid Homo sapiens
119 GP9 2815
Affinity Capture-MS Homo sapiens
120 PSAT1 29968
Co-fractionation Homo sapiens
121 TXNDC15  
Affinity Capture-MS Homo sapiens
122 OPALIN  
Affinity Capture-MS Homo sapiens
123 SEMA7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 MFSD4  
Affinity Capture-MS Homo sapiens
125 P2RX1 5023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 TPTE  
Affinity Capture-MS Homo sapiens
127 LDLRAD1  
Affinity Capture-MS Homo sapiens
128 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
129 TRIM9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 RNASE10  
Two-hybrid Homo sapiens
131 WIF1 11197
Affinity Capture-MS Homo sapiens
132 IFNLR1  
Affinity Capture-MS Homo sapiens
133 ESR1  
Affinity Capture-MS Homo sapiens
134 PPM1G 5496
Affinity Capture-MS Homo sapiens
135 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 BRICD5  
Two-hybrid Homo sapiens
137 ARSF 416
Affinity Capture-MS Homo sapiens
138 SEC62 7095
Affinity Capture-MS Homo sapiens
139 FAM69A 388650
Affinity Capture-MS Homo sapiens
140 ZNRF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 RAB7A 7879
Proximity Label-MS Homo sapiens
142 CYB5R3 1727
Affinity Capture-MS Homo sapiens
143 VDAC3 7419
Co-fractionation Homo sapiens
144 PCDHB3  
Affinity Capture-MS Homo sapiens
145 CLEC4E  
Affinity Capture-MS Homo sapiens
146 LRRTM3  
Affinity Capture-MS Homo sapiens
147 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SGPL1 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid catabolism TAS Reactome
Sphingolipid metabolism TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here