Gene description for MBOAT7
Gene name membrane bound O-acyltransferase domain containing 7
Gene symbol MBOAT7
Other names/aliases BB1
LENG4
LPIAT
LRC4
MBOA7
OACT7
hMBOA-7
Species Homo sapiens
 Database cross references - MBOAT7
ExoCarta ExoCarta_79143
Vesiclepedia VP_79143
Entrez Gene 79143
HGNC 15505
MIM 606048
UniProt Q96N66  
 MBOAT7 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for MBOAT7
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    protein binding GO:0005515 IPI
    O-acyltransferase activity GO:0008374 IDA
    O-acyltransferase activity GO:0008374 IMP
    O-acyltransferase activity GO:0008374 ISS
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
    lysophospholipid acyltransferase activity GO:0071617 IBA
    lysophospholipid acyltransferase activity GO:0071617 ISS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 IBA
    phosphatidylinositol biosynthetic process GO:0006661 IMP
    ventricular system development GO:0021591 IEA
    layer formation in cerebral cortex GO:0021819 ISS
    lipid modification GO:0030258 IBA
    phosphatidylinositol acyl-chain remodeling GO:0036149 IDA
    phosphatidylinositol acyl-chain remodeling GO:0036149 IMP
    phosphatidylinositol acyl-chain remodeling GO:0036149 TAS
    phosphatidylcholine acyl-chain remodeling GO:0036151 IDA
    regulation of triglyceride metabolic process GO:0090207 IMP
    regulation of triglyceride metabolic process GO:0090207 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 IBA
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 IDA
 Experiment description of studies that identified MBOAT7 in exosomes
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MBOAT7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ILDR2  
Affinity Capture-MS Homo sapiens
2 STARD3NL 83930
Affinity Capture-MS Homo sapiens
3 TPRA1  
Affinity Capture-MS Homo sapiens
4 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KDELR3 11015
Affinity Capture-MS Homo sapiens
6 SGCA  
Affinity Capture-MS Homo sapiens
7 GJD3  
Proximity Label-MS Homo sapiens
8 Tmed2 56334
Affinity Capture-MS Mus musculus
9 C5AR1  
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 Spast  
Affinity Capture-MS Mus musculus
12 VAPA 9218
Affinity Capture-MS Homo sapiens
13 MARCKS 4082
Proximity Label-MS Homo sapiens
14 LAMP3  
Proximity Label-MS Homo sapiens
15 SLC39A4 55630
Affinity Capture-MS Homo sapiens
16 CMTM8  
Affinity Capture-MS Homo sapiens
17 ACKR2  
Affinity Capture-MS Homo sapiens
18 TMEM184A  
Affinity Capture-MS Homo sapiens
19 HSD17B11 51170
Proximity Label-MS Homo sapiens
20 UBC 7316
Reconstituted Complex Homo sapiens
21 LAMP2 3920
Proximity Label-MS Homo sapiens
22 DNAJC25 548645
Proximity Label-MS Homo sapiens
23 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
24 FFAR1  
Affinity Capture-MS Homo sapiens
25 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 APEX1 328
Affinity Capture-RNA Homo sapiens
27 TM2D3  
Affinity Capture-MS Homo sapiens
28 B3GAT1  
Proximity Label-MS Homo sapiens
29 SGPL1 8879
Affinity Capture-MS Homo sapiens
30 ACAP2 23527
Affinity Capture-MS Homo sapiens
31 STS 412
Affinity Capture-MS Homo sapiens
32 STOM 2040
Affinity Capture-MS Homo sapiens
33 STX8 9482
Affinity Capture-MS Homo sapiens
34 SSR1 6745
Proximity Label-MS Homo sapiens
35 COX7B2  
Affinity Capture-MS Homo sapiens
36 ATP2A3 489
Affinity Capture-MS Homo sapiens
37 LMBRD1 55788
Affinity Capture-MS Homo sapiens
38 METTL7A 25840
Proximity Label-MS Homo sapiens
39 CD200R1 131450
Affinity Capture-MS Homo sapiens
40 NDUFS1 4719
Affinity Capture-MS Homo sapiens
41 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 FAM20C 56975
Affinity Capture-MS Homo sapiens
43 ADRA1A  
Two-hybrid Homo sapiens
44 NUP155 9631
Proximity Label-MS Homo sapiens
45 KIAA1715 80856
Proximity Label-MS Homo sapiens
46 HTR3C  
Affinity Capture-MS Homo sapiens
47 LMNB1 4001
Proximity Label-MS Homo sapiens
48 EMD 2010
Proximity Label-MS Homo sapiens
49 SPTSSA  
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
50 GOLT1B 51026
Affinity Capture-MS Homo sapiens
51 P2RY8  
Affinity Capture-MS Homo sapiens
52 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
53 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RPN1 6184
Proximity Label-MS Homo sapiens
55 P2RY2 5029
Affinity Capture-MS Homo sapiens
56 PML 5371
Affinity Capture-MS Homo sapiens
57 TMEM63A 9725
Affinity Capture-MS Homo sapiens
58 UPK1A 11045
Affinity Capture-MS Homo sapiens
59 ELOVL5 60481
Proximity Label-MS Homo sapiens
60 SEC61B 10952
Proximity Label-MS Homo sapiens
61 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CLRN2  
Affinity Capture-MS Homo sapiens
63 CRELD1 78987
Affinity Capture-MS Homo sapiens
64 SEC63 11231
Proximity Label-MS Homo sapiens
65 TM4SF20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CYB5B 80777
Affinity Capture-MS Homo sapiens
67 APLNR  
Affinity Capture-MS Homo sapiens
68 RPN2 6185
Proximity Label-MS Homo sapiens
69 SIGLECL1  
Affinity Capture-MS Homo sapiens
70 BTNL9  
Affinity Capture-MS Homo sapiens
71 LRRC59 55379
Proximity Label-MS Homo sapiens
72 PANX1 24145
Proximity Label-MS Homo sapiens
73 ADCY9 115
Affinity Capture-MS Homo sapiens
74 Bmpr1a  
Affinity Capture-MS Mus musculus
75 B3GNT3 10331
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SLC35A5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 Rmdn3  
Affinity Capture-MS Mus musculus
78 BCAP31 10134
Proximity Label-MS Homo sapiens
79 HSD3B7 80270
Proximity Label-MS Homo sapiens
80 NR3C1 2908
Proximity Label-MS Homo sapiens
81 STIM1 6786
Proximity Label-MS Homo sapiens
82 ARF6 382
Proximity Label-MS Homo sapiens
83 HTR2C  
Affinity Capture-MS Homo sapiens
84 YIPF5 81555
Affinity Capture-MS Homo sapiens
85 LPAR1 1902
Affinity Capture-MS Homo sapiens
86 TBXA2R 6915
Affinity Capture-MS Homo sapiens
87 PKN2 5586
Affinity Capture-MS Homo sapiens
88 SYP  
Affinity Capture-MS Homo sapiens
89 TMEM173  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
90 MAPK6  
Two-hybrid Homo sapiens
91 BDKRB1 623
Two-hybrid Homo sapiens
92 SOAT1 6646
Affinity Capture-MS Homo sapiens
93 RPA3 6119
Proximity Label-MS Homo sapiens
94 ATP2A1 487
Proximity Label-MS Homo sapiens
95 RAB9A 9367
Proximity Label-MS Homo sapiens
96 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 TM2D2 83877
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 GPR37  
Affinity Capture-MS Homo sapiens
99 SLC22A4 6583
Affinity Capture-MS Homo sapiens
100 PTGFR 5737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 LBR 3930
Affinity Capture-MS Homo sapiens
103 CLPP 8192
Proximity Label-MS Homo sapiens
104 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
105 EDNRA 1909
Affinity Capture-MS Homo sapiens
106 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 ATP5J 522
Affinity Capture-MS Homo sapiens
108 C3AR1  
Affinity Capture-MS Homo sapiens
109 OPALIN  
Affinity Capture-MS Homo sapiens
110 TSPAN15 23555
Affinity Capture-MS Homo sapiens
111 UNC93B1 81622
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
112 ERGIC1 57222
Proximity Label-MS Homo sapiens
113 CD36 948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 C1orf162  
Affinity Capture-MS Homo sapiens
115 GPR52  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 HM13 81502
Affinity Capture-MS Homo sapiens
117 SLC5A8 160728
Affinity Capture-MS Homo sapiens
118 SEC62 7095
Proximity Label-MS Homo sapiens
119 SPPL2B 56928
Affinity Capture-MS Homo sapiens
120 RAB7A 7879
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
121 POMK  
Affinity Capture-MS Homo sapiens
122 RAB11A 8766
Proximity Label-MS Homo sapiens
123 SLC24A3  
Affinity Capture-MS Homo sapiens
124 SERBP1 26135
Affinity Capture-MS Homo sapiens
125 GGA2 23062
Proximity Label-MS Homo sapiens
126 ATP5C1 509
Affinity Capture-MS Homo sapiens
127 RAB5C 5878
Proximity Label-MS Homo sapiens
128 CD44 960
Affinity Capture-MS Homo sapiens
129 GOLM1 51280
Affinity Capture-MS Homo sapiens
130 CCDC47 57003
Affinity Capture-MS Homo sapiens
131 CKAP4 10970
Proximity Label-MS Homo sapiens
132 COX4I1 1327
Affinity Capture-MS Homo sapiens
133 S1PR4  
Affinity Capture-MS Homo sapiens
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