Gene description for CD36
Gene name CD36 molecule (thrombospondin receptor)
Gene symbol CD36
Other names/aliases BDPLT10
CHDS7
FAT
GP3B
GP4
GPIV
PASIV
SCARB3
Species Homo sapiens
 Database cross references - CD36
ExoCarta ExoCarta_948
Vesiclepedia VP_948
Entrez Gene 948
HGNC 1663
MIM 173510
UniProt P16671  
 CD36 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
Breast milk 17641064    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 20224111    
Urine 20224111    
 Gene ontology annotations for CD36
Molecular Function
    transforming growth factor beta binding GO:0050431 ISS
    lipid binding GO:0008289 IDA
    lipoteichoic acid receptor activity GO:0070892 IEA
    high-density lipoprotein particle binding GO:0008035 IEA
    thrombospondin receptor activity GO:0070053 ISS
    lipoprotein particle binding GO:0071813 IDA
    low-density lipoprotein particle binding GO:0030169 IDA
    low-density lipoprotein receptor activity GO:0005041 TAS
Biological Process
    negative regulation of transcription factor import into nucleus GO:0042992 IEA
    defense response to Gram-positive bacterium GO:0050830 IEA
    receptor-mediated endocytosis GO:0006898 TAS
    cellular response to lipoteichoic acid GO:0071223 IEA
    small molecule metabolic process GO:0044281 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    response to stilbenoid GO:0035634 IEA
    cGMP-mediated signaling GO:0019934 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    low-density lipoprotein particle mediated signaling GO:0055096 IEA
    positive regulation of blood microparticle formation GO:2000334 IEA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    phagocytosis, recognition GO:0006910 IEA
    cellular response to bacterial lipopeptide GO:0071221 IEA
    regulation of removal of superoxide radicals GO:2000121 IEA
    positive regulation of blood coagulation GO:0030194 IEA
    long-chain fatty acid import GO:0044539 IDA
    positive regulation of phagocytosis, engulfment GO:0060100 IEA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    apoptotic cell clearance GO:0043277 IEA
    negative regulation of growth of symbiont in host GO:0044130 IEA
    blood coagulation GO:0007596 TAS
    cholesterol transport GO:0030301 ISS
    positive regulation of interleukin-6 production GO:0032755 IEA
    low-density lipoprotein particle clearance GO:0034383 IMP
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    lipid metabolic process GO:0006629 NAS
    cellular lipid metabolic process GO:0044255 TAS
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to hydroperoxide GO:0071447 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    plasma lipoprotein particle clearance GO:0034381 ISS
    lipoprotein transport GO:0042953 TAS
    platelet activation GO:0030168 TAS
    cell adhesion GO:0007155 IEA
    innate immune response GO:0045087 TAS
    cell surface receptor signaling pathway GO:0007166 IEA
    platelet degranulation GO:0002576 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    lipid storage GO:0019915 IMP
    nitric oxide mediated signal transduction GO:0007263 IDA
    plasma membrane long-chain fatty acid transport GO:0015911 IDA
    positive regulation of reactive oxygen species metabolic process GO:2000379 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    positive regulation of interleukin-12 production GO:0032735 IEA
    positive regulation of cholesterol storage GO:0010886 IEA
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
Subcellular Localization
    phagocytic vesicle GO:0045335 TAS
    extracellular space GO:0005615 IDA
    integral component of plasma membrane GO:0005887 TAS
    membrane GO:0016020 TAS
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 ISS
    platelet alpha granule membrane GO:0031092 TAS
    external side of plasma membrane GO:0009897 IEA
    endocytic vesicle membrane GO:0030666 TAS
    membrane raft GO:0045121 IEA
 Experiment description of studies that identified CD36 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
6
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 77
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
11
Experiment ID 78
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Bladder cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
 Protein-protein interactions for CD36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THBS1 7057
Invivo Homo sapiens
Invivo Homo sapiens
2 ITGB1 3688
Invivo Homo sapiens
3 LDLR 3949
Reconstituted Complex Homo sapiens
4 COL1A1 1277
Invitro Homo sapiens
5 ITGB3 3690
Invivo Homo sapiens
6 YES1 7525
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
7 COL1A2 1278
Invitro Homo sapiens
8 SRC 6714
Invivo Homo sapiens
9 VLDLR 7436
Reconstituted Complex Homo sapiens
10 ITGA2B 3674
Invivo Homo sapiens
11 CD9 928
Invivo Homo sapiens
12 FYN 2534
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
13 ITGA6 3655
Invivo Homo sapiens
14 MATK  
Affinity Capture-Western Homo sapiens
15 HSD11B1 3290
Reconstituted Complex Homo sapiens
16 LYN 4067
Affinity Capture-Western Homo sapiens
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