Gene description for CD9
Gene name CD9 molecule
Gene symbol CD9
Other names/aliases BTCC-1
DRAP-27
MIC3
MRP-1
TSPAN-29
TSPAN29
Species Homo sapiens
 Database cross references - CD9
ExoCarta ExoCarta_928
Entrez Gene 928
HGNC 1709
MIM 143030
UniProt P21926  
 CD9 identified in exosomes derived from the following tissue/cell type
Amniotic fluid 17700640    
Ascites 21601258    
B cells 21505438    
Bladder cancer cells 20224111    
Bone marrow cells 15908444    
Breast milk 21235781    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 22740476    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25330373    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Dendritic cells 21505438    
Endothelial cells 26027894    
Malignant ascites 21601258    
Malignant ascites 19188015    
Mast cells 15908444    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 25669974    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Neuroblastoma cells 26022510    
Neuroblastoma cells 26022510    
Ovarian cancer cells 19188015    
Ovarian cancer cells 19188015    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 21763319    
Plasma 15908444    
Plasma 21235781    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pleural effusions 21601258    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25857301    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Saliva 21235781    
Serum 21601258    
Serum 21601258    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Serum 25844599    
Squamous carcinoma cells 20124223    
T cells 21505438    
Tabecular meshwork cells 21362503    
Thymus 23844026    
Urine 15326289    
Urine 17700640    
Urine 19056867    
Urine 20224111    
Urine 20224111    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CD9
Molecular Function
    protein binding GO:0005515 IPI
    integrin binding GO:0005178 IEA
Biological Process
    fusion of sperm to egg plasma membrane GO:0007342 TAS
    single fertilization GO:0007338 TAS
    response to water deprivation GO:0009414 IEA
    paranodal junction assembly GO:0030913 ISS
    platelet degranulation GO:0002576 TAS
    platelet activation GO:0030168 TAS
    oligodendrocyte development GO:0014003 IEA
    movement of cell or subcellular component GO:0006928 IDA
    brain development GO:0007420 IEA
    blood coagulation GO:0007596 TAS
    cell adhesion GO:0007155 IDA
    multicellular organism reproduction GO:0032504 TAS
    negative regulation of cell proliferation GO:0008285 IEA
Subcellular Localization
    external side of plasma membrane GO:0009897 IEA
    extracellular space GO:0005615 IDA
    extracellular vesicle GO:1903561 IDA
    integral component of plasma membrane GO:0005887 NAS
    plasma membrane GO:0005886 TAS
    apical plasma membrane GO:0016324 IEA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    platelet alpha granule membrane GO:0031092 TAS
    endocytic vesicle membrane GO:0030666 TAS
    focal adhesion GO:0005925 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CD9 in exosomes
1
Experiment ID 58
ISEV standards
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name KI
Publication year 2007
Sample Amniotic fluid
Sample name Amniotic fluid
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 110
ISEV standards
EM|IEM
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD81|CD9|HLA-DR|EpCAM
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name GO
Publication year 2011
Sample Ascites
Sample name Ascites - Liver cirrhosis
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.04-1.10 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
3
Experiment ID 116
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21505438    
Organism Homo sapiens
Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name NATCOM
Publication year 2011
Sample B cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
Microarray
4
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 42
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 124
ISEV standards
IEM|EM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|CD9|CD81
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21235781    
Organism Homo sapiens
Experiment description Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name JTM
Publication year 2011
Sample Breast milk
Sample name Breast milk - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
7
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 201
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 223
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25330373    
Organism Homo sapiens
Experiment description Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures
Authors Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ
Journal name PLoS One
Publication year 2014
Sample Colorectal cancer cells
Sample name LIM1863
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Immunobeads (EpCAM)
Immunobeads (A33)
Flotation density -
Molecules identified in the study miRNA
Protein
Methods used in the study RT-PCR
Western blotting
14
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 285
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
18
Experiment ID 117
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21505438    
Organism Homo sapiens
Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name NATCOM
Publication year 2011
Sample Dendritic cells
Sample name Dendritic cells
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
Microarray
19
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
20
Experiment ID 108
ISEV standards
EM|IEM
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD81|CD9|EpCAM
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name GO
Publication year 2011
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM, CD24)
Flotation density 1.04-1.15 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
21
Experiment ID 131
ISEV standards
EV Biophysical techniques
HSP70
EV Cytosolic markers
EpCAM|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
22
Experiment ID 41
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|MHCI|CD9|CD81|TFRC|LAMP2
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Mast cells
Sample name HMC-1
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
23
Experiment ID 254
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
Western blotting
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
27
Experiment ID 261
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD81|LAMP2|CD9
EV Membrane markers
EV Negative markers
Y
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25669974    
Organism Homo sapiens
Experiment description Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites
Authors Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally
Journal name Oncotarget
Publication year 2014
Sample Mesenchymal stem cells
Sample name hMSCs
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Lipids
Metabolites
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
Sequencing
28
Experiment ID 219
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 220
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 221
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 239
ISEV standards
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD9
EV Membrane markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26022510    
Organism Homo sapiens
Experiment description Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name J Extracell Vesicles
Publication year 2015
Sample Neuroblastoma cells
Sample name N2a OptiMEM - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density 1.10-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 242
ISEV standards
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26022510    
Organism Homo sapiens
Experiment description Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name J Extracell Vesicles
Publication year 2015
Sample Neuroblastoma cells
Sample name N2a Pre-spun - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density 1.10-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 132
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name CL
Publication year 2009
Sample Ovarian cancer cells
Sample name OVMz
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
FACS
34
Experiment ID 133
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name CL
Publication year 2009
Sample Ovarian cancer cells
Sample name SKOV3ip
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
FACS
35
Experiment ID 211
ISEV standards