Gene ontology annotations for CD9
Experiment description of studies that identified CD9 in exosomes
1
Experiment ID
58
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
17700640
Organism
Homo sapiens
Experiment description
CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors
Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name
KI
Publication year
2007
Sample
Amniotic fluid
Sample name
Amniotic fluid
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting
2
Experiment ID
110
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD81|CD9|HLA-DR|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Ascites
Sample name
Ascites - Liver cirrhosis
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.04-1.10 g/mL
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Immunoelctron microscopy
3
Experiment ID
116
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
B cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
4
Experiment ID
76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
5
Experiment ID
42
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Bone marrow cells
Sample name
PBMC
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
6
Experiment ID
124
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Breast milk
Sample name
Breast milk - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
7
Experiment ID
20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
8
Experiment ID
21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
9
Experiment ID
201
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22740476
Organism
Homo sapiens
Experiment description
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name
Electrophoresis
Publication year
2012
Sample
Colorectal cancer cells
Sample name
SW480
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
209
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
223
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25330373
Organism
Homo sapiens
Experiment description
Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures
Authors
Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ
Journal name
PLoS One
Publication year
2014
Sample
Colorectal cancer cells
Sample name
LIM1863
Isolation/purification methods
Differential centrifugation Ultracentrifugation Immunobeads (EpCAM) Immunobeads (A33)
Flotation density
-
Molecules identified in the study
miRNA Protein
Methods used in the study
RT-PCR Western blotting
14
Experiment ID
282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
15
Experiment ID
283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
16
Experiment ID
285
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
17
Experiment ID
286
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
18
Experiment ID
117
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
Dendritic cells
Sample name
Dendritic cells
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
19
Experiment ID
226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26027894
Organism
Homo sapiens
Experiment description
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name
Journal of Extracellular Vesicles
Publication year
2015
Sample
Endothelial cells
Sample name
HMEC-1
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA
Methods used in the study
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting
20
Experiment ID
108
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD81|CD9|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Malignant ascites
Sample name
Malignant ascites - Ovarian cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (EPCAM, CD24)
Flotation density
1.04-1.15 g/mL
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Immunoelctron microscopy
21
Experiment ID
131
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
EpCAM|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting FACS
PubMed ID
19188015
Organism
Homo sapiens
Experiment description
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name
CL
Publication year
2009
Sample
Malignant ascites
Sample name
Malignant ascites - Ovarian cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 mg/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Cytofluorographic analysis Annexin-V-FITC Western blotting FACS
22
Experiment ID
41
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|MHCI|CD9|CD81|TFRC|LAMP2
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Mast cells
Sample name
HMC-1
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
23
Experiment ID
254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
MNT-1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
126
ISEV standards
✘
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra] Western blotting
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
27
Experiment ID
261
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD81|LAMP2|CD9
EV Membrane markers
✘
EV Negative markers
✔
Y
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25669974
Organism
Homo sapiens
Experiment description
Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites
Authors
Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally
Journal name
Oncotarget
Publication year
2014
Sample
Mesenchymal stem cells
Sample name
hMSCs
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA Lipids Metabolites
Methods used in the study
Western blotting Mass spectrometry RT-PCR Sequencing
28
Experiment ID
219
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
C666-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
220
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP69
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
30
Experiment ID
221
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP460
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
31
Experiment ID
239
ISEV standards
✘
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD9
EV Membrane markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26022510
Organism
Homo sapiens
Experiment description
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name
J Extracell Vesicles
Publication year
2015
Sample
Neuroblastoma cells
Sample name
N2a OptiMEM - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
1.10-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
242
ISEV standards
✘
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
26022510
Organism
Homo sapiens
Experiment description
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE.
Journal name
J Extracell Vesicles
Publication year
2015
Sample
Neuroblastoma cells
Sample name
N2a Pre-spun - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
1.10-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
33
Experiment ID
132
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
19188015
Organism
Homo sapiens
Experiment description
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name
CL
Publication year
2009
Sample
Ovarian cancer cells
Sample name
OVMz
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 mg/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Cytofluorographic analysis Annexin-V-FITC FACS
34
Experiment ID
133
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
19188015
Organism
Homo sapiens
Experiment description
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name
CL
Publication year
2009
Sample
Ovarian cancer cells
Sample name
SKOV3ip
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 mg/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Cytofluorographic analysis Annexin-V-FITC FACS
35
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
36
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
103
ISEV standards
✘
EV Biophysical techniques
✔
Alix|SDCBP
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21763319
Organism
Homo sapiens
Experiment description
Characterization of soluble and exosomal forms of the EGFR released from pancreatic cancer cells.
Authors
Adamczyk KA, Klein-Scory S, Tehrani MM, Warnken U, Schmiegel W, Schnolzer M, Schwarte-Waldhoff I.
Journal name
LS
Publication year
2011
Sample
Pancreatic cancer cells
Sample name
Panc1
Isolation/purification methods
Differential centrifugation Filtration Ultrafiltration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
38
Experiment ID
40
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.15-1.27 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
39
Experiment ID
123
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
40
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
41
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
42
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
43
Experiment ID
109
ISEV standards
✘
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD9|MHCII|HLA-DR|CD24|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Pleural effusions
Sample name
Pleural effusions - Breast cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (EPCAM)
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting
44
Experiment ID
142
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
45
Experiment ID
143
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
46
Experiment ID
144
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
C4-2 - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
47
Experiment ID
145
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
CD10
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
Hosseini-Beheshti E, Guns ES.
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
C4-2 - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
48
Experiment ID
245
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - Ethanol treated (control)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids Protein
Methods used in the study
Western blotting Mass spectrometry
49
Experiment ID
246
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - sn-1-O-hexadecylglycerol treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids
Methods used in the study
Western blotting Mass spectrometry
50
Experiment ID
250
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25857301
Organism
Homo sapiens
Experiment description
Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors
Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - expressing DIAPH3 hRNA
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
51
Experiment ID
275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Flow cytometric analysis Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
52
Experiment ID
274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Flow cytometric analysis Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
53
Experiment ID
66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
54
Experiment ID
122
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Saliva
Sample name
Saliva - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
55
Experiment ID
111
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD9|CD24|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Serum
Sample name
Serum - Breast cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (CD24)
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting
56
Experiment ID
112
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD9|CD24|EpCAM
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21601258
Organism
Homo sapiens
Experiment description
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name
GO
Publication year
2011
Sample
Serum
Sample name
Serum - Normal
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting
57
Experiment ID
276
ISEV standards
✘
EV Biophysical techniques
✔
Alix|RAB5A
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Serum
Sample name
Serum - Docetaxel resistant castration resistant prostate cancer patient 1
Isolation/purification methods
Filtration Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
58
Experiment ID
277
ISEV standards
✘
EV Biophysical techniques
✔
Alix|RAB5A
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Serum
Sample name
Serum - Docetaxel resistant castration resistant prostate cancer patient 2
Isolation/purification methods
Filtration Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
59
Experiment ID
278
ISEV standards
✘
EV Biophysical techniques
✔
Alix|RAB5A
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Serum
Sample name
Serum - Docetaxel resistant castration resistant prostate cancer patient 3
Isolation/purification methods
Filtration Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
60
Experiment ID
281
ISEV standards
✘
EV Biophysical techniques
✔
Alix|RAB5A
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Serum
Sample name
Serum - Docetaxel sensitive castration resistant prostate cancer patient 6
Isolation/purification methods
Filtration Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
61
Experiment ID
191
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
62
Experiment ID
115
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
T cells
Sample name
J77
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
63
Experiment ID
118
ISEV standards
✘
EV Biophysical techniques
✔
SDCBP|GAPDH
EV Cytosolic markers
✔
CD9|CD81|FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21362503
Organism
Homo sapiens
Experiment description
Protein profile of exosomes from trabecular meshwork cells.
Authors
Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL.
Journal name
JOP
Publication year
2011
Sample
Tabecular meshwork cells
Sample name
Tabecular meshwork cells
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.07-1.2 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
64
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
65
Experiment ID
13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
Pisitkun T, Shen RF, Knepper MA
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
66
Experiment ID
57
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
17700640
Organism
Homo sapiens
Experiment description
CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors
Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name
KI
Publication year
2007
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.05-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS
67
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
68
Experiment ID
77
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
69
Experiment ID
78
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Urine
Sample name
Urine - Bladder cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
70
Experiment ID
193
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
71
Experiment ID
194
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
72
Experiment ID
195
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
73
Experiment ID
196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
74
Experiment ID
197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for CD9
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ITGB1
3688
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
2
CD81
975
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
3
CD36
948
Invivo
Homo sapiens
4
ADAM2
Affinity Capture-MS
Homo sapiens
5
ITGA3
3675
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
6
KIT
3815
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
7
ITGA5
3678
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
CD53
963
Affinity Capture-Western
Homo sapiens
9
CD63
967
Affinity Capture-Western
Homo sapiens
Invivo
Homo sapiens
10
PTGFRN
5738
Invitro
Homo sapiens
Invivo
Homo sapiens
Affinity Capture-Western
Homo sapiens
11
PRKCA
5578
Invivo
Homo sapiens
12
CD19
930
Invivo
Homo sapiens
13
SERPINH1
871
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
14
TSPAN4
7106
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
15
CD59
966
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
16
CD82
3732
Affinity Capture-Western
Homo sapiens
17
IGSF8
93185
Affinity Capture-Western
Homo sapiens
18
HBEGF
Invivo
Homo sapiens
19
CD46
4179
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which CD9 is involved