Gene ontology annotations for CD9 |
|
Experiment description of studies that identified CD9 in exosomes |
1 |
Experiment ID |
58 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
17700640
|
Organism |
Homo sapiens |
Experiment description |
CD24 is a marker of exosomes secreted into urine and amniotic fluid. |
Authors |
Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P |
Journal name |
KI
|
Publication year |
2007 |
Sample |
Amniotic fluid |
Sample name |
Amniotic fluid |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.08-1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
2 |
Experiment ID |
110 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
HSP70
|
EV Cytosolic markers |
✔
CD81|CD9|HLA-DR|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
21601258
|
Organism |
Homo sapiens |
Experiment description |
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. |
Authors |
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P. |
Journal name |
GO
|
Publication year |
2011 |
Sample |
Ascites |
Sample name |
Ascites - Liver cirrhosis |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.04-1.10 g/mL
|
Molecules identified in the study |
Protein miRNA |
Methods used in the study |
Western blotting Immunoelctron microscopy |
|
|
3 |
Experiment ID |
116 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21505438
|
Organism |
Homo sapiens |
Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
Authors |
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F. |
Journal name |
NATCOM
|
Publication year |
2011 |
Sample |
B cells |
Sample name |
Raji |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein miRNA |
Methods used in the study |
Mass spectrometry Microarray |
|
|
4 |
Experiment ID |
76 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|GAPDH|HSP90
|
EV Cytosolic markers |
✔
CD81|CD9|CD63|LAMP1|MHCI
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry Western blotting FACS
|
PubMed ID |
20224111
|
Organism |
Homo sapiens |
Experiment description |
Proteomics analysis of bladder cancer exosomes. |
Authors |
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A. |
Journal name |
MCP
|
Publication year |
2010 |
Sample |
Bladder cancer cells |
Sample name |
HT1376 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS |
|
|
5 |
Experiment ID |
42 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
|
EV Membrane markers |
✔
CANX
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
15908444
|
Organism |
Homo sapiens |
Experiment description |
Exosomal-like vesicles are present in human blood plasma. |
Authors |
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C |
Journal name |
INTIMM
|
Publication year |
2005 |
Sample |
Bone marrow cells |
Sample name |
PBMC |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting |
|
|
6 |
Experiment ID |
124 |
ISEV standards |
✔
IEM|EM
|
EV Biophysical techniques |
✔
HSC70
|
EV Cytosolic markers |
✔
CD63|CD9|CD81
|
EV Membrane markers |
✔
CANX
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
21235781
|
Organism |
Homo sapiens |
Experiment description |
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages. |
Authors |
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H. |
Journal name |
JTM
|
Publication year |
2011 |
Sample |
Breast milk |
Sample name |
Breast milk - Normal |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
FACS Immunoelectron Microscopy Western blotting |
|
|
7 |
Experiment ID |
20 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP90
|
EV Cytosolic markers |
✔
CD63|CD81|LAMP1
|
EV Membrane markers |
✔
GOLGA2|cytochrome c
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17956143
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
Authors |
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS |
Journal name |
JPR
|
Publication year |
2007 |
Sample |
Colorectal cancer cells |
Sample name |
HT29 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Diafiltration |
Flotation density |
1.16 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting |
|
|
8 |
Experiment ID |
21 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
19837982
|
Organism |
Homo sapiens |
Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
Authors |
Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson |
Journal name |
MCP
|
Publication year |
2009 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1215 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.10-1.12 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [Orbitrap] Western blotting |
|
|
9 |
Experiment ID |
201 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
22740476
|
Organism |
Homo sapiens |
Experiment description |
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. |
Authors |
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ. |
Journal name |
Electrophoresis
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
SW480 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
10 |
Experiment ID |
207 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DKO-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
11 |
Experiment ID |
208 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
Dks-8 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
12 |
Experiment ID |
209 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
TSG101|HSP70
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✔
VDAC
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23161513
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2012 |
Sample |
Colorectal cancer cells |
Sample name |
DLD-1 |
Isolation/purification methods |
Differential centrifugation Filtration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
13 |
Experiment ID |
223 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
25330373
|
Organism |
Homo sapiens |
Experiment description |
Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures |
Authors |
Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ |
Journal name |
PLoS One
|
Publication year |
2014 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Immunobeads (EpCAM) Immunobeads (A33) |
Flotation density |
-
|
Molecules identified in the study |
miRNA Protein |
Methods used in the study |
RT-PCR Western blotting |
|
|
14 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
15 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
16 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
17 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
18 |
Experiment ID |
117 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21505438
|
Organism |
Homo sapiens |
Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
Authors |
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F. |
Journal name |
NATCOM
|
Publication year |
2011 |
Sample |
Dendritic cells |
Sample name |
Dendritic cells |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein miRNA |
Methods used in the study |
Mass spectrometry Microarray |
|
|
19 |
Experiment ID |
226 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✔
CD9|FLOT1
|
EV Membrane markers |
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
26027894
|
Organism |
Homo sapiens |
Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
Journal name |
Journal of Extracellular Vesicles
|
Publication year |
2015 |
Sample |
Endothelial cells |
Sample name |
HMEC-1 |
Isolation/purification methods |
Differential ultracentrifugation Sucrose density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA |
Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting |
|
|
20 |
Experiment ID |
108 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
HSP70
|
EV Cytosolic markers |
✔
CD81|CD9|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
21601258
|
Organism |
Homo sapiens |
Experiment description |
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. |
Authors |
Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P. |
Journal name |
GO
|
Publication year |
2011 |
Sample |
Malignant ascites |
Sample name |
Malignant ascites - Ovarian cancer |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (EPCAM, CD24) |
Flotation density |
1.04-1.15 g/mL
|
Molecules identified in the study |
Protein miRNA |
Methods used in the study |
Western blotting Immunoelctron microscopy |
|
|
21 |
Experiment ID |
131 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
HSP70
|
EV Cytosolic markers |
✔
EpCAM|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting FACS
|
PubMed ID |
19188015
|
Organism |
Homo sapiens |
Experiment description |
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes |
Authors |
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P. |
Journal name |
CL
|
Publication year |
2009 |
Sample |
Malignant ascites |
Sample name |
Malignant ascites - Ovarian cancer |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.08-1.14 mg/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Cytofluorographic analysis Annexin-V-FITC Western blotting FACS |
|
|
22 |
Experiment ID |
41 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|MHCI|CD9|CD81|TFRC|LAMP2
|
EV Membrane markers |
✔
CANX
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
FACS Western blotting
|
PubMed ID |
15908444
|
Organism |
Homo sapiens |
Experiment description |
Exosomal-like vesicles are present in human blood plasma. |
Authors |
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C |
Journal name |
INTIMM
|
Publication year |
2005 |
Sample |
Mast cells |
Sample name |
HMC-1 |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting FACS Immunoelectron Microscopy |
|
|
23 |
Experiment ID |
254 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
MNT-1 |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
24 |
Experiment ID |
259 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
A375M |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
25 |
Experiment ID |
260 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
1205Lu |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
26 |
Experiment ID |
126 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra] Western blotting
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
27 |
Experiment ID |
261 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD81|LAMP2|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
Y
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
25669974
|
Organism |
Homo sapiens |
Experiment description |
Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites |
Authors |
Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally |
Journal name |
Oncotarget
|
Publication year |
2014 |
Sample |
Mesenchymal stem cells |
Sample name |
hMSCs |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein miRNA Lipids Metabolites |
Methods used in the study |
Western blotting Mass spectrometry RT-PCR Sequencing |
|
|
28 |
Experiment ID |
219 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
25857718
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
Journal name |
Int J Cancer
|
Publication year |
2015 |
Sample |
Nasopharyngeal carcinoma cells |
Sample name |
C666-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.17-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
29 |
Experiment ID |
220 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
25857718
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
Journal name |
Int J Cancer
|
Publication year |
2015 |
Sample |
Nasopharyngeal carcinoma cells |
Sample name |
NP69 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.17-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
30 |
Experiment ID |
221 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
25857718
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins |
Authors |
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P |
Journal name |
Int J Cancer
|
Publication year |
2015 |
Sample |
Nasopharyngeal carcinoma cells |
Sample name |
NP460 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.17-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
31 |
Experiment ID |
239 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✔
CANX
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
26022510
|
Organism |
Homo sapiens |
Experiment description |
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
Authors |
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE. |
Journal name |
J Extracell Vesicles
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
N2a OptiMEM - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
1.10-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
32 |
Experiment ID |
242 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✔
CANX
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting
|
PubMed ID |
26022510
|
Organism |
Homo sapiens |
Experiment description |
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles |
Authors |
Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE. |
Journal name |
J Extracell Vesicles
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
N2a Pre-spun - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
1.10-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
33 |
Experiment ID |
132 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
FACS
|
PubMed ID |
19188015
|
Organism |
Homo sapiens |
Experiment description |
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes |
Authors |
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P. |
Journal name |
CL
|
Publication year |
2009 |
Sample |
Ovarian cancer cells |
Sample name |
OVMz |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.08-1.14 mg/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Cytofluorographic analysis Annexin-V-FITC FACS |
|
|
34 |
Experiment ID |
133 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
FACS
|
PubMed ID |
19188015
|
Organism |
Homo sapiens |
Experiment description |
Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes |
Authors |
Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P. |
Journal name |
CL
|
Publication year |
2009 |
Sample |
Ovarian cancer cells |
Sample name |
SKOV3ip |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.08-1.14 mg/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Cytofluorographic analysis Annexin-V-FITC FACS |
|
|
| | |