Gene description for ITGA2B
Gene name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Gene symbol ITGA2B
Other names/aliases BDPLT16
BDPLT2
CD41
CD41B
GP2B
GPIIb
GT
GTA
HPA3
PPP1R93
Species Homo sapiens
 Database cross references - ITGA2B
ExoCarta ExoCarta_3674
Entrez Gene 3674
HGNC 6138
MIM 607759
UniProt P08514  
 ITGA2B identified in exosomes derived from the following tissue/cell type
Bone marrow cells 15908444    
Plasma 15908444    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for ITGA2B
Molecular Function
    protein binding GO:0005515 IPI
    extracellular matrix binding GO:0050840 IEA
    metal ion binding GO:0046872 IEA
    fibrinogen binding GO:0070051 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    platelet aggregation GO:0070527 IEA
    axon guidance GO:0007411 TAS
    extracellular matrix organization GO:0030198 TAS
    cell-matrix adhesion GO:0007160 IEA
    platelet degranulation GO:0002576 TAS
    platelet activation GO:0030168 TAS
    integrin-mediated signaling pathway GO:0007229 IDA
    cell adhesion GO:0007155 TAS
    blood coagulation GO:0007596 TAS
    positive regulation of leukocyte migration GO:0002687 IEA
Subcellular Localization
    integral component of plasma membrane GO:0005887 TAS
    external side of plasma membrane GO:0009897 IEA
    cell surface GO:0009986 IDA
    extracellular exosome GO:0070062 IDA
    blood microparticle GO:0072562 IDA
    focal adhesion GO:0005925 IEA
    plasma membrane GO:0005886 TAS
    platelet alpha granule membrane GO:0031092 TAS
    integrin complex GO:0008305 IEA
 Experiment description of studies that identified ITGA2B in exosomes
1
Experiment ID 42
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 40
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.15-1.27 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
3
Experiment ID 44
ISEV standards
IEM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
8
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for ITGA2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD36 948
Invivo Homo sapiens
2 ITGB3 3690
Invivo Homo sapiens
Invitro Homo sapiens
3 AUP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 COL1A2 1278
Invivo Homo sapiens
5 GRB2 2885
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
6 VWF 7450
Invivo Homo sapiens
Invitro Homo sapiens
7 F2 2147
Reconstituted Complex Homo sapiens
8 FGA 2243
Invitro Homo sapiens
9 CIB1 10519
Invitro Homo sapiens
Two-hybrid Homo sapiens
10 COL2A1 1280
Reconstituted Complex Homo sapiens
11 CLNS1A 1207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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