Gene description for PLA2G4A
Gene name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Gene symbol PLA2G4A
Other names/aliases PLA2G4
cPLA2-alpha
Species Homo sapiens
 Database cross references - PLA2G4A
ExoCarta ExoCarta_5321
Vesiclepedia VP_5321
Entrez Gene 5321
HGNC 9035
MIM 600522
UniProt P47712  
 PLA2G4A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PLA2G4A
Molecular Function
    lysophospholipase activity GO:0004622 IDA
    phospholipase A2 activity GO:0004623 IDA
    phospholipase A2 activity GO:0004623 NAS
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IDA
    O-acyltransferase activity GO:0008374 IDA
    phosphatidylinositol-5-phosphate binding GO:0010314 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    calcium-dependent phospholipase A2 activity GO:0047498 IBA
    calcium-dependent phospholipase A2 activity GO:0047498 IDA
    calcium-dependent phospholipase A2 activity GO:0047498 IMP
    calcium-independent phospholipase A2 activity GO:0047499 IDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    ceramide 1-phosphate binding GO:1902387 IDA
Biological Process
    prostaglandin biosynthetic process GO:0001516 IDA
    prostaglandin biosynthetic process GO:0001516 IEA
    prostaglandin biosynthetic process GO:0001516 IMP
    positive regulation of T-helper 1 type immune response GO:0002827 IEA
    glycerol metabolic process GO:0006071 IEA
    monoacylglycerol biosynthetic process GO:0006640 IDA
    platelet activating factor biosynthetic process GO:0006663 NAS
    icosanoid metabolic process GO:0006690 NAS
    positive regulation of platelet activation GO:0010572 IMP
    arachidonate metabolic process GO:0019369 IDA
    arachidonate metabolic process GO:0019369 IEA
    leukotriene biosynthetic process GO:0019370 IMP
    positive regulation of prostaglandin secretion GO:0032308 IEA
    phosphatidylglycerol catabolic process GO:0034478 IDA
    phosphatidylcholine catabolic process GO:0034638 IDA
    phosphatidylcholine acyl-chain remodeling GO:0036151 IDA
    regulation of cell population proliferation GO:0042127 IEA
    positive regulation of macrophage activation GO:0043032 IEA
    glycerophospholipid catabolic process GO:0046475 IBA
    arachidonate secretion GO:0050482 IEA
    establishment of localization in cell GO:0051649 IEA
    cellular response to antibiotic GO:0071236 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    nucleus GO:0005634 IBA
    nuclear envelope GO:0005635 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial inner membrane GO:0005743 TAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified PLA2G4A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PLA2G4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AHSA1 10598
Affinity Capture-MS Homo sapiens
2 RELA 5970
Two-hybrid Homo sapiens
3 PHLPP1  
Proximity Label-MS Homo sapiens
4 ITGA2B 3674
Reconstituted Complex Homo sapiens
5 CCNA2 890
Affinity Capture-Western Homo sapiens
6 SHBG 6462
Two-hybrid Homo sapiens
7 EHD1 10938
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
8 TP53 7157
Affinity Capture-MS Homo sapiens
9 LRRC59 55379
Affinity Capture-MS Homo sapiens
10 RTN3 10313
Co-fractionation Homo sapiens
11 RUVBL2 10856
Co-fractionation Homo sapiens
12 SUGT1 10910
Affinity Capture-MS Homo sapiens
13 MAPK1 5594
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
14 ITGB3 3690
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 ANXA1 301
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 MAPK3 5595
Affinity Capture-Western Homo sapiens
17 PPP5C 5536
Affinity Capture-MS Homo sapiens
18 CD9 928
Co-fractionation Homo sapiens
19 S100A10 6281
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
21 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
22 RSF1  
Co-fractionation Homo sapiens
23 NCF1  
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 ANKRD28 23243
Co-fractionation Homo sapiens
25 NCF2 4688
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 NXF1 10482
Affinity Capture-RNA Homo sapiens
27 CKB 1152
Co-fractionation Homo sapiens
28 CDK2 1017
Affinity Capture-Western Homo sapiens
29 FKBP5 2289
Affinity Capture-MS Homo sapiens
30 MOV10 4343
Affinity Capture-RNA Homo sapiens
31 JAK1 3716
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 S100A11 6282
Affinity Capture-Western Homo sapiens
33 PRKCA 5578
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
34 CSNK2A2 1459
Co-fractionation Homo sapiens
35 DHPS 1725
Co-fractionation Homo sapiens
36 KAT5  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 RPAP3 79657
Affinity Capture-MS Homo sapiens
38 ALOX12 239
Affinity Capture-Western Homo sapiens
39 MYBL2 4605
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 SIRT2 22933
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
41 CAP1 10487
Two-hybrid Homo sapiens
42 XAGE1E  
Two-hybrid Homo sapiens
43 STAT3 6774
Affinity Capture-MS Homo sapiens
44 DNAJC17  
Affinity Capture-MS Homo sapiens
45 ANXA2 302
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
46 VIM 7431
Affinity Capture-Western Homo sapiens
47 Eif3e 16341
Affinity Capture-MS Mus musculus
48 UBXN1 51035
Two-hybrid Homo sapiens
49 C17orf59 54785
Two-hybrid Homo sapiens
50 Ccdc9  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here