Gene description for PLA2G4A
Gene name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Gene symbol PLA2G4A
Other names/aliases PLA2G4
cPLA2-alpha
Species Homo sapiens
 Database cross references - PLA2G4A
ExoCarta ExoCarta_5321
Entrez Gene 5321
HGNC 9035
MIM 600522
UniProt P47712  
 PLA2G4A identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PLA2G4A
Molecular Function
    phospholipase A2 activity GO:0004623 TAS
    calcium ion binding GO:0005509 IDA
    calcium-dependent phospholipid binding GO:0005544 IDA
    calcium-dependent phospholipase A2 activity GO:0047498 EXP
    lysophospholipase activity GO:0004622 IEA
Biological Process
    arachidonic acid metabolic process GO:0019369 TAS
    icosanoid metabolic process GO:0006690 NAS
    phosphatidylcholine acyl-chain remodeling GO:0036151 TAS
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 TAS
    phosphatidylserine acyl-chain remodeling GO:0036150 TAS
    phosphatidylglycerol acyl-chain remodeling GO:0036148 TAS
    phospholipid metabolic process GO:0006644 TAS
    phosphatidylinositol acyl-chain remodeling GO:0036149 TAS
    phospholipid catabolic process GO:0009395 IEA
    arachidonic acid secretion GO:0050482 IEA
    glycerophospholipid biosynthetic process GO:0046474 TAS
    platelet activation GO:0030168 TAS
    small molecule metabolic process GO:0044281 TAS
    cardiolipin acyl-chain remodeling GO:0035965 TAS
    cellular response to antibiotic GO:0071236 IEA
    platelet activating factor biosynthetic process GO:0006663 NAS
    blood coagulation GO:0007596 TAS
    regulation of cell proliferation GO:0042127 IEA
    icosanoid biosynthetic process GO:0046456 IEA
    phosphatidic acid biosynthetic process GO:0006654 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    Golgi apparatus GO:0005794 IEA
    mitochondrial inner membrane GO:0005743 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
 Experiment description of studies that identified PLA2G4A in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PLA2G4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HTATIP  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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