Gene description for JAK1
Gene name Janus kinase 1
Gene symbol JAK1
Other names/aliases JAK1A
JAK1B
JTK3
Species Homo sapiens
 Database cross references - JAK1
ExoCarta ExoCarta_3716
Entrez Gene 3716
HGNC 6190
MIM 147795
UniProt P23458  
 JAK1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Melanoma cells 22635005    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for JAK1
Molecular Function
    protein tyrosine kinase activity GO:0004713 TAS
    protein phosphatase binding GO:0019903 IPI
    growth hormone receptor binding GO:0005131 ISS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    CCR5 chemokine receptor binding GO:0031730 IEA
Biological Process
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    response to antibiotic GO:0046677 IDA
    regulation of cell proliferation GO:0042127 IBA
    interleukin-2-mediated signaling pathway GO:0038110 IDA
    cytokine-mediated signaling pathway GO:0019221 TAS
    cell differentiation GO:0030154 IBA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    type I interferon signaling pathway GO:0060337 TAS
    cell migration GO:0016477 IBA
    intracellular signal transduction GO:0035556 IEA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    protein phosphorylation GO:0006468 TAS
    innate immune response GO:0045087 IBA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    endomembrane system GO:0012505 IEA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified JAK1 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
4
Experiment ID 200
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|TSG101|Alix|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D.
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for JAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTK 695
Affinity Capture-Western Homo sapiens
2 PTPN11 5781
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Biochemical Activity Homo sapiens
3 OSMR 9180
Invivo Homo sapiens
4 GRB2 2885
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 STAT5B 6777
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 PTPN6 5777
Biochemical Activity Homo sapiens
7 STAT5A 6776
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 TSHR 7253
Invivo Homo sapiens
Invitro Homo sapiens
9 ELF3 1999
Affinity Capture-MS Homo sapiens
10 PLAUR 5329
Invivo Homo sapiens
Invitro Homo sapiens
11 SHB  
Reconstituted Complex Homo sapiens
12 BRCA1 672
Affinity Capture-Western Homo sapiens
13 TNFRSF1A 7132
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
14 ABL1 25
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
15 IFNGR1 3459
Invivo Homo sapiens
16 IGF1R 3480
Reconstituted Complex Homo sapiens
17 IL5RA  
Affinity Capture-Western Homo sapiens
18 ELP2 55250
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 ABL2  
Affinity Capture-Western Homo sapiens
20 IL21R  
Affinity Capture-Western Homo sapiens
21 IL4R  
Invivo Homo sapiens
Invitro Homo sapiens
22 JAK3 3718
Invivo Homo sapiens
23 PRMT5 10419
Reconstituted Complex Homo sapiens
24 IL6ST 3572
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 CSF3R  
Affinity Capture-Western Homo sapiens
26 TYK2 7297
Affinity Capture-Western Homo sapiens
27 ARRB1 408
Affinity Capture-MS Homo sapiens
28 PTK2B 2185
Affinity Capture-Western Homo sapiens
29 IL10RA  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
30 STAM2 10254
Invitro Homo sapiens
Biochemical Activity Homo sapiens
31 IL27RA 9466
Affinity Capture-Western Homo sapiens
32 RAF1 5894
Invivo Homo sapiens
33 IL2RB  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 GHR  
Invivo Homo sapiens
Invitro Homo sapiens
35 STAT3 6774
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 SYK 6850
Invitro Homo sapiens
38 TEC 7006
Affinity Capture-Western Homo sapiens
39 IL9R  
Affinity Capture-Western Homo sapiens
40 IRS1 3667
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
41 PIK3R1 5295
Invivo Homo sapiens
42 ATIC 471
Affinity Capture-MS Homo sapiens
43 IRS2 8660
Affinity Capture-Western Homo sapiens
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