Gene description for S100B
Gene name S100 calcium binding protein B
Gene symbol S100B
Other names/aliases NEF
S100
S100-B
S100beta
Species Homo sapiens
 Database cross references - S100B
ExoCarta ExoCarta_6285
Vesiclepedia VP_6285
Entrez Gene 6285
HGNC 10500
MIM 176990
UniProt P04271  
 S100B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for S100B
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IPI
    S100 protein binding GO:0044548 IBA
    S100 protein binding GO:0044548 IPI
    tau protein binding GO:0048156 ISS
    calcium-dependent protein binding GO:0048306 IBA
    calcium-dependent protein binding GO:0048306 IDA
    RAGE receptor binding GO:0050786 IBA
    RAGE receptor binding GO:0050786 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    axonogenesis GO:0007409 TAS
    central nervous system development GO:0007417 TAS
    learning or memory GO:0007611 ISS
    memory GO:0007613 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of neuron differentiation GO:0045666 IEA
    regulation of neuronal synaptic plasticity GO:0048168 IEA
    sympathetic neuron projection extension GO:0097490 ISS
    adaptive thermogenesis GO:1990845 ISS
Subcellular Localization
    ruffle GO:0001726 IDA
    extracellular region GO:0005576 ISS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    neuronal cell body GO:0043025 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified S100B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for S100B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRIM2 5558
Affinity Capture-MS Homo sapiens
2 NOP14 8602
Affinity Capture-MS Homo sapiens
3 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
4 GTF2F1 2962
Affinity Capture-MS Homo sapiens
5 ORC2  
Affinity Capture-MS Homo sapiens
6 SENP2 59343
Affinity Capture-MS Homo sapiens
7 POLDIP3 84271
Affinity Capture-MS Homo sapiens
8 HMCES  
Affinity Capture-MS Homo sapiens
9 ZNF714  
Affinity Capture-MS Homo sapiens
10 MRPL30  
Affinity Capture-MS Homo sapiens
11 UTP11L  
Affinity Capture-MS Homo sapiens
12 S100A6 6277
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 MORC4  
Affinity Capture-MS Homo sapiens
14 RCOR2 283248
Affinity Capture-MS Homo sapiens
15 FDXR 2232
Affinity Capture-MS Homo sapiens
16 CALM1 801
Reconstituted Complex Homo sapiens
17 ANXA6 309
Reconstituted Complex Homo sapiens
18 ARID3B  
Affinity Capture-MS Homo sapiens
19 SYDE2  
Affinity Capture-MS Homo sapiens
20 FOXO4  
Affinity Capture-MS Homo sapiens
21 AGAP3 116988
Affinity Capture-MS Homo sapiens
22 ZNF3  
Affinity Capture-MS Homo sapiens
23 MLLT11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 S100A4 6275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 ERF  
Affinity Capture-MS Homo sapiens
26 ZBTB10 65986
Affinity Capture-MS Homo sapiens
27 BCL7B  
Affinity Capture-MS Homo sapiens
28 METTL25  
Affinity Capture-MS Homo sapiens
29 ZBTB21  
Affinity Capture-MS Homo sapiens
30 NDRG1 10397
Affinity Capture-Western Homo sapiens
31 SALL3  
Affinity Capture-MS Homo sapiens
32 NR2C2  
Affinity Capture-MS Homo sapiens
33 CEP112  
Affinity Capture-MS Homo sapiens
34 IQGAP1 8826
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
35 PGM1 5236
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 PIF1  
Affinity Capture-MS Homo sapiens
37 SRC 6714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 ATAD5  
Affinity Capture-MS Homo sapiens
39 ZNF451 26036
Affinity Capture-MS Homo sapiens
40 ORC6  
Affinity Capture-MS Homo sapiens
41 FAM207A  
Affinity Capture-MS Homo sapiens
42 ZBTB5  
Affinity Capture-MS Homo sapiens
43 PEAK1 79834
Affinity Capture-MS Homo sapiens
44 INTS6 26512
Affinity Capture-MS Homo sapiens
45 XRCC3  
Affinity Capture-MS Homo sapiens
46 METTL18  
Affinity Capture-MS Homo sapiens
47 SDCBP 6386
Two-hybrid Homo sapiens
48 FBXW5  
Two-hybrid Homo sapiens
49 CCDC102A 92922
Affinity Capture-MS Homo sapiens
50 ALAS1  
Affinity Capture-MS Homo sapiens
51 ATAD3B 83858
Affinity Capture-MS Homo sapiens
52 NSUN2 54888
Affinity Capture-MS Homo sapiens
53 SPG21 51324
Two-hybrid Homo sapiens
54 S100A2 6273
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 POLD1 5424
Affinity Capture-MS Homo sapiens
56 VAV1 7409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 UTP14A 10813
Affinity Capture-MS Homo sapiens
58 SGOL2 151246
Affinity Capture-MS Homo sapiens
59 CAPZA1 829
Reconstituted Complex Homo sapiens
60 TNKS1BP1 85456
Affinity Capture-MS Homo sapiens
61 OFD1 8481
Affinity Capture-MS Homo sapiens
62 FOPNL  
Reconstituted Complex Homo sapiens
63 LOC81691 81691
Affinity Capture-MS Homo sapiens
64 ADTRP  
Two-hybrid Homo sapiens
65 MAPT  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
66 S100A11 6282
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
67 NOC4L 79050
Affinity Capture-MS Homo sapiens
68 SCML2  
Affinity Capture-MS Homo sapiens
69 C5orf42  
Affinity Capture-MS Homo sapiens
70 S100B 6285
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
71 HEMK1  
Affinity Capture-MS Homo sapiens
72 PML 5371
Affinity Capture-MS Homo sapiens
73 DCLRE1B  
Affinity Capture-MS Homo sapiens
74 DNAJC30  
Two-hybrid Homo sapiens
75 AHCTF1 25909
Affinity Capture-MS Homo sapiens
76 POLR3GL  
Affinity Capture-MS Homo sapiens
77 IQGAP2 10788
Affinity Capture-MS Homo sapiens
78 CT45A5  
Two-hybrid Homo sapiens
79 ZBED1  
Affinity Capture-MS Homo sapiens
80 ZNF516 9658
Affinity Capture-MS Homo sapiens
81 ZBTB4 57659
Affinity Capture-MS Homo sapiens
82 CDK17 5128
Affinity Capture-MS Homo sapiens
83 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
84 CISD3 284106
Affinity Capture-MS Homo sapiens
85 S100P 6286
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
86 GFER  
Affinity Capture-MS Homo sapiens
87 MAP7 9053
Affinity Capture-MS Homo sapiens
88 C11orf57  
Two-hybrid Homo sapiens
89 CTU1 90353
Affinity Capture-MS Homo sapiens
90 FAM178A  
Affinity Capture-MS Homo sapiens
91 AHNAK 79026
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
92 ASB6 140459
Affinity Capture-MS Homo sapiens
93 TIGD4 201798
Affinity Capture-MS Homo sapiens
94 RICTOR 253260
Affinity Capture-MS Homo sapiens
95 DCAF13 25879
Affinity Capture-MS Homo sapiens
96 ZC3HC1  
Affinity Capture-MS Homo sapiens
97 NHSL1  
Affinity Capture-MS Homo sapiens
98 COX6B1 1340
Affinity Capture-MS Homo sapiens
99 MTFR1  
Affinity Capture-MS Homo sapiens
100 TTF2 8458
Affinity Capture-MS Homo sapiens
101 PUS7 54517
Affinity Capture-MS Homo sapiens
102 KDM2B 84678
Affinity Capture-MS Homo sapiens
103 KDM3B  
Affinity Capture-MS Homo sapiens
104 S100Z  
Two-hybrid Homo sapiens
105 MTMR12 54545
Affinity Capture-MS Homo sapiens
106 JAK1 3716
Affinity Capture-MS Homo sapiens
107 CNTROB  
Affinity Capture-MS Homo sapiens
108 ZNF507  
Affinity Capture-MS Homo sapiens
109 MAP3K4 4216
Affinity Capture-MS Homo sapiens
110 CDC45  
Affinity Capture-MS Homo sapiens
111 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
112 FYTTD1  
Affinity Capture-MS Homo sapiens
113 SENP5  
Affinity Capture-MS Homo sapiens
114 ZNF136  
Affinity Capture-MS Homo sapiens
115 PLEKHG3 26030
Affinity Capture-MS Homo sapiens
116 C18orf21  
Affinity Capture-MS Homo sapiens
117 IMPA1 3612
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
118 MDM2  
Reconstituted Complex Homo sapiens
119 CCDC8  
Affinity Capture-MS Homo sapiens
120 UBE3B 89910
Affinity Capture-MS Homo sapiens
121 SALL2  
Affinity Capture-MS Homo sapiens
122 FOXO3  
Affinity Capture-MS Homo sapiens
123 TERF2  
Affinity Capture-MS Homo sapiens
124 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
125 TTC28 23331
Affinity Capture-MS Homo sapiens
126 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
127 STK38 11329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
128 FLYWCH2  
Affinity Capture-MS Homo sapiens
129 PLCD3 113026
Affinity Capture-MS Homo sapiens
130 PRIM1  
Affinity Capture-MS Homo sapiens
131 CDK18 5129
Affinity Capture-MS Homo sapiens
132 ZNF536  
Affinity Capture-MS Homo sapiens
133 CCDC106  
Affinity Capture-MS Homo sapiens
134 ZNF764 92595
Affinity Capture-MS Homo sapiens
135 SALL1 6299
Affinity Capture-MS Homo sapiens
136 LSM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 S100A9 6280
Two-hybrid Homo sapiens
138 MAP7D3 79649
Affinity Capture-MS Homo sapiens
139 RAB11FIP1 80223
Affinity Capture-MS Homo sapiens
140 RAVER1 125950
Affinity Capture-MS Homo sapiens
141 ATAD3A 55210
Affinity Capture-MS Homo sapiens
142 HIC2 23119
Affinity Capture-MS Homo sapiens
143 RPL23 9349
Affinity Capture-MS Homo sapiens
144 MYO1E 4643
Affinity Capture-MS Homo sapiens
145 NRIP1 8204
Affinity Capture-MS Homo sapiens
146 TXNIP 10628
Affinity Capture-MS Homo sapiens
147 KIAA1551  
Affinity Capture-MS Homo sapiens
148 S100A1 6271
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
149 ZNF709  
Affinity Capture-MS Homo sapiens
150 CDKN2AIP  
Affinity Capture-MS Homo sapiens
151 PHF8  
Affinity Capture-MS Homo sapiens
152 BCL7A  
Affinity Capture-MS Homo sapiens
153 FAM64A 54478
Affinity Capture-MS Homo sapiens
154 IMP4  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which S100B is involved
PathwayEvidenceSource
Advanced glycosylation endproduct receptor signaling TAS Reactome
Advanced glycosylation endproduct receptor signaling IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
DDX58/IFIH1-mediated induction of interferon-alpha/beta TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling TAS Reactome
Interleukin-1 signaling TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
Signal Transduction TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
TAK1-dependent IKK and NF-kappa-B activation TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRAF6 mediated NF-kB activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome





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