Gene description for S100B
Gene name S100 calcium binding protein B
Gene symbol S100B
Other names/aliases NEF
S100
S100-B
S100beta
Species Homo sapiens
 Database cross references - S100B
ExoCarta ExoCarta_6285
Vesiclepedia VP_6285
Entrez Gene 6285
HGNC 10500
MIM 176990
UniProt P04271  
 S100B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for S100B
Molecular Function
    RAGE receptor binding GO:0050786 IPI
    calcium-dependent protein binding GO:0048306 IDA
    calcium ion binding GO:0005509 NAS
    protein homodimerization activity GO:0042803 IPI
    zinc ion binding GO:0008270 ISS
    protein binding GO:0005515 IPI
    tau protein binding GO:0048156 ISS
    identical protein binding GO:0042802 IPI
    S100 protein binding GO:0044548 IPI
Biological Process
    regulation of neuronal synaptic plasticity GO:0048168 IEA
    regulation of cell shape GO:0008360 IEA
    memory GO:0007613 IEA
    response to glucocorticoid GO:0051384 IEA
    learning or memory GO:0007611 ISS
    axonogenesis GO:0007409 TAS
    central nervous system development GO:0007417 TAS
    cell proliferation GO:0008283 TAS
    cellular response to hypoxia GO:0071456 IEA
    negative regulation of skeletal muscle cell differentiation GO:2001015 IEA
    response to methylmercury GO:0051597 IEA
    astrocyte differentiation GO:0048708 IEA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IDA
    positive regulation of apoptotic process GO:0043065 IEA
    long-term synaptic potentiation GO:0060291 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    innate immune response GO:0045087 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    neuronal cell body GO:0043025 IEA
    ruffle GO:0001726 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular space GO:0005615 IEA
    cytoplasm GO:0005737 ISS
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified S100B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for S100B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 S100A6 6277
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 HSGT1  
Affinity Capture-MS Homo sapiens
3 S100B 6285
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 AHNAK 79026
Invitro Homo sapiens
Invivo Homo sapiens
5 PGM1 5236
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
6 NDRG1 10397
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
8 AGER  
Invivo Homo sapiens
9 S100A1 6271
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 MAPT  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
11 ANXA6 309
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 CAPZA1 829
Reconstituted Complex Homo sapiens
13 S100A11 6282
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 GFAP 2670
Affinity Capture-MS Homo sapiens
15 TP53 7157
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 CACYBP 27101
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
17 IQGAP1 8826
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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