Gene description for PLCD3
Gene name phospholipase C, delta 3
Gene symbol PLCD3
Other names/aliases PLC-delta-3
Species Homo sapiens
 Database cross references - PLCD3
ExoCarta ExoCarta_113026
Vesiclepedia VP_113026
Entrez Gene 113026
HGNC 9061
MIM 608795
UniProt Q8N3E9  
 PLCD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PLCD3
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    lipid catabolic process GO:0016042 IEA
    intracellular signal transduction GO:0035556 IEA
    regulation of cell population proliferation GO:0042127 IEA
    labyrinthine layer blood vessel development GO:0060716 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cleavage furrow GO:0032154 IEA
 Experiment description of studies that identified PLCD3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLCD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 UBE2O 63893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NOS1AP  
Affinity Capture-MS Homo sapiens
4 TRAT1 50852
Affinity Capture-MS Homo sapiens
5 C18orf25 147339
Affinity Capture-MS Homo sapiens
6 VPRBP 9730
Affinity Capture-MS Homo sapiens
7 ARL15 54622
Affinity Capture-MS Homo sapiens
8 FBXL19  
Affinity Capture-MS Homo sapiens
9 GPALPP1  
Affinity Capture-MS Homo sapiens
10 BRD2  
Affinity Capture-MS Homo sapiens
11 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
12 AFF1  
Affinity Capture-MS Homo sapiens
13 WDR26 80232
Affinity Capture-MS Homo sapiens
14 ELL  
Affinity Capture-MS Homo sapiens
15 VANGL2  
Affinity Capture-MS Homo sapiens
16 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
17 RNPS1 10921
Affinity Capture-MS Homo sapiens
18 INSRR 3645
Affinity Capture-MS Homo sapiens
19 IFT74 80173
Affinity Capture-MS Homo sapiens
20 AFF4 27125
Affinity Capture-MS Homo sapiens
21 NOLC1 9221
Affinity Capture-MS Homo sapiens
22 RANBP10 57610
Affinity Capture-MS Homo sapiens
23 IFT52 51098
Affinity Capture-MS Homo sapiens
24 TNIK 23043
Affinity Capture-MS Homo sapiens
25 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
26 IFT81 28981
Affinity Capture-MS Homo sapiens
27 LRRC46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PPP1R15B  
Affinity Capture-MS Homo sapiens
29 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MLLT1 4298
Affinity Capture-MS Homo sapiens
31 GEMIN2 8487
Affinity Capture-MS Homo sapiens
32 KIF14 9928
Affinity Capture-MS Homo sapiens
33 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 IGDCC4  
Affinity Capture-MS Homo sapiens
35 CXXC1  
Affinity Capture-MS Homo sapiens
36 AP3M1 26985
Affinity Capture-MS Homo sapiens
37 FAM96A  
Affinity Capture-MS Homo sapiens
38 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
39 MCC 4163
Affinity Capture-MS Homo sapiens
40 WDR70 55100
Affinity Capture-MS Homo sapiens
41 RTF1 23168
Affinity Capture-MS Homo sapiens
42 DDX20 11218
Affinity Capture-MS Homo sapiens
43 RAD18  
Affinity Capture-MS Homo sapiens
44 S100B 6285
Affinity Capture-MS Homo sapiens
45 UBTF 7343
Affinity Capture-MS Homo sapiens
46 GPBP1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 Coro1c 23790
Affinity Capture-MS Mus musculus
48 AP3B1 8546
Affinity Capture-MS Homo sapiens
49 BRD4 23476
Affinity Capture-MS Homo sapiens
50 EFNB3 1949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 AHCYL1 10768
Affinity Capture-MS Homo sapiens
52 CHD9 80205
Affinity Capture-MS Homo sapiens
53 TICAM2  
Affinity Capture-MS Homo sapiens
54 ANLN 54443
Affinity Capture-MS Homo sapiens
55 EPDR1 54749
Affinity Capture-MS Homo sapiens
56 S100P 6286
Affinity Capture-MS Homo sapiens
57 FTL 2512
Affinity Capture-MS Homo sapiens
58 BTNL9  
Affinity Capture-MS Homo sapiens
59 OSBPL6  
Affinity Capture-MS Homo sapiens
60 CD6 923
Affinity Capture-MS Homo sapiens
61 SHQ1  
Affinity Capture-MS Homo sapiens
62 METTL21B  
Affinity Capture-MS Homo sapiens
63 NAF1  
Affinity Capture-MS Homo sapiens
64 SDC2 6383
Affinity Capture-MS Homo sapiens
65 ELL3  
Affinity Capture-MS Homo sapiens
66 SETD1A 9739
Affinity Capture-MS Homo sapiens
67 KCNE3  
Affinity Capture-MS Homo sapiens
68 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ANKRD52 283373
Affinity Capture-MS Homo sapiens
70 SYNPO 11346
Affinity Capture-MS Homo sapiens
71 SPEN 23013
Affinity Capture-MS Homo sapiens
72 AP3M2  
Affinity Capture-MS Homo sapiens
73 RELT  
Affinity Capture-MS Homo sapiens
74 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
75 DHPS 1725
Affinity Capture-MS Homo sapiens
76 HIP1 3092
Affinity Capture-MS Homo sapiens
77 CHD8 57680
Affinity Capture-MS Homo sapiens
78 SF3B3 23450
Affinity Capture-MS Homo sapiens
79 KBTBD7  
Affinity Capture-MS Homo sapiens
80 BRD3 8019
Affinity Capture-MS Homo sapiens
81 AP3S1 1176
Affinity Capture-MS Homo sapiens
82 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SKIL  
Two-hybrid Homo sapiens
84 ZRANB2 9406
Affinity Capture-MS Homo sapiens
85 CWC27 10283
Affinity Capture-MS Homo sapiens
86 OPALIN  
Affinity Capture-MS Homo sapiens
87 GEMIN6  
Affinity Capture-MS Homo sapiens
88 KANK1  
Affinity Capture-MS Homo sapiens
89 EFNB1 1947
Affinity Capture-MS Homo sapiens
90 MAGEA9  
Affinity Capture-MS Homo sapiens
91 DDX58 23586
Affinity Capture-RNA Homo sapiens
92 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
94 EAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 ZCCHC10  
Affinity Capture-MS Homo sapiens
96 SSSCA1 10534
Affinity Capture-MS Homo sapiens
97 MLLT3 4300
Affinity Capture-MS Homo sapiens
98 TCOF1 6949
Affinity Capture-MS Homo sapiens
99 UBXN6 80700
Affinity Capture-MS Homo sapiens
100 CWC22 57703
Affinity Capture-MS Homo sapiens
101 VANGL1 81839
Affinity Capture-MS Homo sapiens
102 AP3D1 8943
Affinity Capture-MS Homo sapiens
103 FGF1  
Affinity Capture-MS Homo sapiens
104 DCAF5  
Affinity Capture-MS Homo sapiens
105 TRPC4AP  
Affinity Capture-MS Homo sapiens
106 ARIH1 25820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PLCD3 is involved
PathwayEvidenceSource
Inositol phosphate metabolism TAS Reactome
Metabolism TAS Reactome
Synthesis of IP3 and IP4 in the cytosol TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here