Gene description for DDX20
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Gene symbol DDX20
Other names/aliases DP103
GEMIN3
Species Homo sapiens
 Database cross references - DDX20
ExoCarta ExoCarta_11218
Vesiclepedia VP_11218
Entrez Gene 11218
HGNC 2743
MIM 606168
UniProt Q9UHI6  
 DDX20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DDX20
Molecular Function
    protein domain specific binding GO:0019904 IEA
    ATP-dependent RNA helicase activity GO:0004004 TAS
    ATP binding GO:0005524 IEA
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
Biological Process
    RNA processing GO:0006396 TAS
    RNA secondary structure unwinding GO:0010501 IBA
    regulation of steroid biosynthetic process GO:0050810 IEA
    ncRNA metabolic process GO:0034660 TAS
    positive regulation of apoptotic process GO:0043065 ISS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    spliceosomal tri-snRNP complex assembly GO:0000244 TAS
    gene expression GO:0010467 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    oogenesis GO:0048477 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 TAS
    transcriptional repressor complex GO:0017053 IEA
    Gemini of coiled bodies GO:0097504 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    SMN-Sm protein complex GO:0034719 IDA
    SMN complex GO:0032797 IDA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DDX20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DDX20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GEMIN2 8487
Invivo Homo sapiens
Invitro Homo sapiens
2 PHB2 11331
Affinity Capture-MS Homo sapiens
3 PRRC2A 7916
Two-hybrid Homo sapiens
4 SMN1 6606
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
5 SNRPE 6635
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 PPP4R2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 GSK3B 2932
Affinity Capture-MS Homo sapiens
8 GAR1 54433
Affinity Capture-Western Homo sapiens
9 AGO2 27161
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 SNRPB 6628
Invivo Homo sapiens
Invitro Homo sapiens
11 GEMIN5 25929
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 PNKP 11284
Affinity Capture-MS Homo sapiens
13 SNRPF 6636
Invivo Homo sapiens
Invitro Homo sapiens
14 SNRPD2 6633
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 GEMIN4 50628
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 GEMIN6  
Affinity Capture-Western Homo sapiens
17 RNPS1 10921
Affinity Capture-MS Homo sapiens
18 SNRPD1 6632
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
19 SNRPG 6637
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 LSM2  
Invivo Homo sapiens
Invitro Homo sapiens
21 FBL 2091
Affinity Capture-Western Homo sapiens
22 PAF53  
Affinity Capture-MS Homo sapiens
23 SNRPD3 6634
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which DDX20 is involved
PathwayEvidenceSource
snRNP Assembly TAS Reactome





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