Gene description for PTPN9
Gene name protein tyrosine phosphatase, non-receptor type 9
Gene symbol PTPN9
Other names/aliases MEG2
PTPMEG2
Species Homo sapiens
 Database cross references - PTPN9
ExoCarta ExoCarta_5780
Vesiclepedia VP_5780
Entrez Gene 5780
HGNC 9661
MIM 600768
UniProt P43378  
 PTPN9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PTPN9
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 TAS
    signal transduction GO:0007165 IBA
    negative regulation of neuron projection development GO:0010977 IDA
    peptidyl-tyrosine dephosphorylation GO:0035335 IDA
    positive regulation of protein localization to plasma membrane GO:1903078 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    neuron projection terminus GO:0044306 IDA
 Experiment description of studies that identified PTPN9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPN9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA3C 7278
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 METTL7A 25840
Proximity Label-MS Homo sapiens
4 LIME1 54923
Two-hybrid Homo sapiens
5 SLC30A8  
Two-hybrid Homo sapiens
6 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FAM26D  
Affinity Capture-MS Homo sapiens
9 COQ3  
Two-hybrid Homo sapiens
10 SMN1 6606
Affinity Capture-MS Homo sapiens
11 VTI1B 10490
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 TTPAL 79183
Affinity Capture-MS Homo sapiens
13 VAMP7 6845
Affinity Capture-Western Homo sapiens
14 TMEM80  
Two-hybrid Homo sapiens
15 ATP5J 522
Two-hybrid Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 TP53BP1 7158
Affinity Capture-MS Homo sapiens
18 ATG9A 79065
Proximity Label-MS Homo sapiens
19 RTN3 10313
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 MDK 4192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 STRAP 11171
Affinity Capture-MS Homo sapiens
22 VSIG8  
Affinity Capture-MS Homo sapiens
23 HSD17B11 51170
Two-hybrid Homo sapiens
24 TMCO2  
Two-hybrid Homo sapiens
25 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SLC39A7 7922
Affinity Capture-MS Homo sapiens
27 WRB 7485
Two-hybrid Homo sapiens
28 TUBA3D 113457
Affinity Capture-MS Homo sapiens
29 MAL2 114569
Two-hybrid Homo sapiens
30 GORAB 92344
Two-hybrid Homo sapiens
31 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
32 PTPN9 5780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NR3C1 2908
Proximity Label-MS Homo sapiens
34 PEX12  
Two-hybrid Homo sapiens
35 GEMIN8 54960
Affinity Capture-MS Homo sapiens
36 CTDP1 9150
Affinity Capture-MS Homo sapiens
37 SLC16A13 201232
Two-hybrid Homo sapiens
38 EBP  
Two-hybrid Homo sapiens
39 HSD17B13  
Two-hybrid Homo sapiens
40 PMPCA 23203
Affinity Capture-MS Homo sapiens
41 PBX3  
Two-hybrid Homo sapiens
42 CPLX4  
Two-hybrid Homo sapiens
43 CREB3L1  
Two-hybrid Homo sapiens
44 STX7 8417
Affinity Capture-Western Homo sapiens
45 NXF1 10482
Affinity Capture-RNA Homo sapiens
46 RAB4A 5867
Proximity Label-MS Homo sapiens
47 SYP  
Two-hybrid Homo sapiens
48 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 TMEM86B  
Two-hybrid Homo sapiens
50 C9orf78 51759
Affinity Capture-MS Homo sapiens
51 GEMIN4 50628
Affinity Capture-MS Homo sapiens
52 FSIP1  
Affinity Capture-MS Homo sapiens
53 STX8 9482
Affinity Capture-Western Homo sapiens
54 DDX20 11218
Affinity Capture-MS Homo sapiens
55 CDR1  
Two-hybrid Homo sapiens
56 MOV10 4343
Affinity Capture-RNA Homo sapiens
57 MYC  
Dosage Lethality Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 PC 5091
Affinity Capture-MS Homo sapiens
60 VCP 7415
Affinity Capture-MS Homo sapiens
61 NTRK1 4914
Affinity Capture-MS Homo sapiens
62 CMTM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 CMTM4  
Two-hybrid Homo sapiens
64 CCR2  
Two-hybrid Homo sapiens
65 ARL6IP1 23204
Two-hybrid Homo sapiens
66 PLIN3 10226
Two-hybrid Homo sapiens
67 BSND  
Two-hybrid Homo sapiens
68 CREB3  
Two-hybrid Homo sapiens
69 GJB1 2705
Two-hybrid Homo sapiens
70 GHR  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
71 ENO1 2023
Affinity Capture-RNA Homo sapiens
72 LZTR1 8216
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ARL13B 200894
Two-hybrid Homo sapiens
74 SMN2 6607
Affinity Capture-MS Homo sapiens
75 MTM1 4534
Affinity Capture-MS Homo sapiens
76 AAR2 25980
Affinity Capture-MS Homo sapiens
77 YIF1A 10897
Two-hybrid Homo sapiens
78 RAD51C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 EYA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 REEP6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
81 TMX2 51075
Two-hybrid Homo sapiens
82 SLC7A1 6541
Two-hybrid Homo sapiens
View the network image/svg+xml



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