Gene description for VTI1B
Gene name vesicle transport through interaction with t-SNAREs 1B
Gene symbol VTI1B
Other names/aliases VTI1
VTI1-LIKE
VTI1L
VTI2
v-SNARE
vti1-rp1
Species Homo sapiens
 Database cross references - VTI1B
ExoCarta ExoCarta_10490
Entrez Gene 10490
HGNC 17793
MIM 603207
UniProt Q9UEU0  
 VTI1B identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for VTI1B
Molecular Function
    protein binding GO:0005515 IPI
    SNAP receptor activity GO:0005484 IBA
    SNARE binding GO:0000149 IDA
    chloride channel inhibitor activity GO:0019869 IDA
Biological Process
    protein targeting to vacuole GO:0006623 IBA
    cell proliferation GO:0008283 TAS
    regulation of protein localization to plasma membrane GO:1903076 IDA
    ER to Golgi vesicle-mediated transport GO:0006888 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    vesicle fusion with Golgi apparatus GO:0048280 IBA
    vesicle-mediated transport GO:0016192 TAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    Golgi to vacuole transport GO:0006896 IBA
    vesicle docking involved in exocytosis GO:0006904 TAS
    autophagosome fusion GO:0000046 IMP
    membrane fusion GO:0061025 TAS
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IDA
    vesicle GO:0031982 IDA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    SNARE complex GO:0031201 IBA
    integral component of membrane GO:0016021 IEA
    synaptic vesicle GO:0008021 ISS
    neuronal cell body GO:0043025 ISS
    endoplasmic reticulum membrane GO:0005789 IBA
    late endosome membrane GO:0031902 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    lysosomal membrane GO:0005765 IDA
 Experiment description of studies that identified VTI1B in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VTI1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STX5A  
Affinity Capture-Western Homo sapiens
2 STX8 9482
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 VAMP4  
Affinity Capture-Western Homo sapiens
4 STX7 8417
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which VTI1B is involved
No pathways found





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