Experiment description of studies that identified TMEM51 in exosomes

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

5 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

Protein-protein interactions for TMEM51

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CCDC12   Affinity Capture-MS Homo sapiens 2 SBDS 51119 Affinity Capture-MS Homo sapiens 3 WWP1 11059 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 SMAGP   Two-hybrid Homo sapiens 5 LOC100507537   Two-hybrid Homo sapiens 6 LYN 4067 Proximity Label-MS Homo sapiens 7 VAMP4 8674 Two-hybrid Homo sapiens 8 RAB35 11021 Proximity Label-MS Homo sapiens 9 TMEM107 84314 Two-hybrid Homo sapiens 10 NINJ2   Two-hybrid Homo sapiens 11 TSNARE1   Two-hybrid Homo sapiens 12 PTER 9317 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 VTI1B 10490 Two-hybrid Homo sapiens 14 OCLN 100506658 Proximity Label-MS Homo sapiens 15 Osgep   Affinity Capture-MS Mus musculus 16 LAMTOR1 55004 Proximity Label-MS Homo sapiens 17 MARCKS 4082 Proximity Label-MS Homo sapiens 18 TMEM254   Two-hybrid Homo sapiens 19 HSPA12A 259217 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 PLCB3 5331 Affinity Capture-MS Homo sapiens 21 GJA1 2697 Proximity Label-MS Homo sapiens 22 CCDC167   Two-hybrid Homo sapiens 23 WWP2 11060 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 SMIM1 388588 Two-hybrid Homo sapiens 25 LAMP1 3916 Proximity Label-MS Homo sapiens 26 CCL4L2   Two-hybrid Homo sapiens 27 PTPRA 5786 Proximity Label-MS Homo sapiens 28 TTI1 9675 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 TMEM14B   Two-hybrid Homo sapiens 30 RTP2   Two-hybrid Homo sapiens 31 TMIE   Two-hybrid Homo sapiens 32 EPS8 2059 Affinity Capture-MS Homo sapiens 33 FAM114A2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 SFT2D2 375035 Two-hybrid Homo sapiens 35 REEP4   Two-hybrid Homo sapiens 36 EBP   Two-hybrid Homo sapiens 37 STX4 6810 Proximity Label-MS Homo sapiens 38 C11orf52 91894 Proximity Label-MS Homo sapiens 39 C14orf180   Two-hybrid Homo sapiens 40 TMEM242   Two-hybrid Homo sapiens 41 SUSD3 203328 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 PUS7 54517 Affinity Capture-MS Homo sapiens 43 KBTBD7   Affinity Capture-MS Homo sapiens 44 METTL15   Affinity Capture-MS Homo sapiens 45 PDCD4 27250 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 ASNA1 439 Two-hybrid Homo sapiens 47 PTPN5   Two-hybrid Homo sapiens 48 ARF6 382 Proximity Label-MS Homo sapiens 49 EPHA2 1969 Proximity Label-MS Homo sapiens 50 B3GAT1   Proximity Label-MS Homo sapiens 51 CDH1 999 Proximity Label-MS Homo sapiens 52 HECW2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 DIRAS3   Proximity Label-MS Homo sapiens 54 CYP4F2   Two-hybrid Homo sapiens 55 RBM14-RBM4 100526737 Affinity Capture-MS Homo sapiens 56 GALC 2581 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 RAB11A 8766 Proximity Label-MS Homo sapiens 58 CUEDC1 404093 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 SLC39A11   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 C4orf3   Two-hybrid Homo sapiens 61 SFXN3 81855 Two-hybrid Homo sapiens 62 LAMP3   Proximity Label-MS Homo sapiens 63 CREB3   Two-hybrid Homo sapiens 64 FNDC3A 22862 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 ZC3HAV1L 92092 Affinity Capture-MS Homo sapiens 66 KRAS 3845 Proximity Label-MS Homo sapiens 67 PLP2 5355 Two-hybrid Homo sapiens 68 SH2D4A 63898 Affinity Capture-MS Homo sapiens 69 NEDD4 4734 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 SEC61G 23480 Two-hybrid Homo sapiens 71 WBP4   Affinity Capture-MS Homo sapiens 72 ITCH 83737 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 CAV1 857 Proximity Label-MS Homo sapiens 74 ELOVL5 60481 Proximity Label-MS Homo sapiens 75 ANKRD13A 88455 Affinity Capture-MS Homo sapiens 76 NEDD4L 23327 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 C20orf24   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 STX6 10228 Proximity Label-MS Homo sapiens 79 TMX2 51075 Two-hybrid Homo sapiens 80 UBE2J1 51465 Two-hybrid Homo sapiens 81 TOMM34 10953 Affinity Capture-MS Homo sapiens View the network

Pathways in which TMEM51 is involved

No pathways found