Gene description for UBE2J1
Gene name ubiquitin-conjugating enzyme E2, J1
Gene symbol UBE2J1
Other names/aliases CGI-76
HSPC153
HSPC205
HSU93243
NCUBE-1
NCUBE1
UBC6
UBC6E
Ubc6p
Species Homo sapiens
 Database cross references - UBE2J1
ExoCarta ExoCarta_51465
Vesiclepedia VP_51465
Entrez Gene 51465
HGNC 17598
MIM 616175
UniProt Q9Y385  
 UBE2J1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UBE2J1
Molecular Function
    protein binding GO:0005515 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IMP
Biological Process
    protein polyubiquitination GO:0000209 IBA
    spermatid development GO:0007286 IEA
    regulation of macrophage cytokine production GO:0010935 IEA
    protein N-linked glycosylation via asparagine GO:0018279 IMP
    regulation of tumor necrosis factor production GO:0032680 IEA
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
    ERAD pathway GO:0036503 IMP
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
 Experiment description of studies that identified UBE2J1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UBE2J1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NME2P1 283458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 SLC10A6  
Two-hybrid Homo sapiens
4 CNTNAP3  
Affinity Capture-MS Homo sapiens
5 DERL2 51009
Affinity Capture-Western Homo sapiens
6 TMEM27 57393
Two-hybrid Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 UBA1 7317
Reconstituted Complex Homo sapiens
12 BRCA1 672
Two-hybrid Homo sapiens
13 LY6G6C  
Two-hybrid Homo sapiens
14 ST3GAL1 6482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 GJD3  
Proximity Label-MS Homo sapiens
16 LAMP2 3920
Proximity Label-MS Homo sapiens
17 DNAJC25 548645
Proximity Label-MS Homo sapiens
18 IL22RA2  
Affinity Capture-MS Homo sapiens
19 CD79A  
Two-hybrid Homo sapiens
20 PTPN1 5770
Proximity Label-MS Homo sapiens
21 SYVN1 84447
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
22 MFSD3  
Two-hybrid Homo sapiens
23 B3GAT1  
Proximity Label-MS Homo sapiens
24 RNF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 GKN1  
Two-hybrid Homo sapiens
26 RAB3B 5865
Proximity Label-MS Homo sapiens
27 SEL1L 6400
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 CLEC2B  
Affinity Capture-MS Homo sapiens
29 OS9 10956
Affinity Capture-MS Homo sapiens
30 SSR1 6745
Proximity Label-MS Homo sapiens
31 LRSAM1 90678
Two-hybrid Homo sapiens
32 DNAJC16  
Proximity Label-MS Homo sapiens
33 SLC25A46 91137
Proximity Label-MS Homo sapiens
34 SEC11C 90701
Two-hybrid Homo sapiens
35 RAB35 11021
Proximity Label-MS Homo sapiens
36 FAF2 23197
Affinity Capture-Western Homo sapiens
37 RNF26  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 LMAN1 3998
Proximity Label-MS Homo sapiens
39 NUP155 9631
Proximity Label-MS Homo sapiens
40 KIAA1715 80856
Proximity Label-MS Homo sapiens
41 PXMP2  
Proximity Label-MS Homo sapiens
42 RAB5A 5868
Proximity Label-MS Homo sapiens
43 DIO2  
Reconstituted Complex Homo sapiens
44 LMNB1 4001
Proximity Label-MS Homo sapiens
45 TMEM14B  
Two-hybrid Homo sapiens
46 ATP1B4  
Affinity Capture-MS Homo sapiens
47 FUT8 2530
Affinity Capture-MS Homo sapiens
48 EMD 2010
Proximity Label-MS Homo sapiens
49 REEP4  
Two-hybrid Homo sapiens
50 PARK2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
51 JAGN1 84522
Two-hybrid Homo sapiens
52 RAB4A 5867
Proximity Label-MS Homo sapiens
53 ASNA1 439
Affinity Capture-Western Homo sapiens
54 LAPTM5 7805
Two-hybrid Homo sapiens
55 VCP 7415
Affinity Capture-Western Homo sapiens
56 GPR182  
Affinity Capture-MS Homo sapiens
57 CREB3  
Two-hybrid Homo sapiens
58 DHFRL1  
Proximity Label-MS Homo sapiens
59 TMX2 51075
Two-hybrid Homo sapiens
60 TMEM56  
Two-hybrid Homo sapiens
61 DERL3  
Affinity Capture-Western Homo sapiens
62 CD63 967
Affinity Capture-MS Homo sapiens
63 ELOVL5 60481
Proximity Label-MS Homo sapiens
64 SEC61B 10952
Proximity Label-MS Homo sapiens
65 TRIM63  
Two-hybrid Homo sapiens
66 YOD1  
Affinity Capture-Western Homo sapiens
67 REEP5 7905
Proximity Label-MS Homo sapiens
68 BIRC2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
69 SEC63 11231
Proximity Label-MS Homo sapiens
70 P2RY2 5029
Affinity Capture-MS Homo sapiens
71 AQP2 359
Two-hybrid Homo sapiens
72 RPN2 6185
Proximity Label-MS Homo sapiens
73 LRRC59 55379
Proximity Label-MS Homo sapiens
74 PANX1 24145
Proximity Label-MS Homo sapiens
75 TM4SF18  
Two-hybrid Homo sapiens
76 TM4SF19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 CHST8 64377
Affinity Capture-MS Homo sapiens
78 PROKR2  
Two-hybrid Homo sapiens
79 BCAP31 10134
Proximity Label-MS Homo sapiens
80 HSD3B7 80270
Proximity Label-MS Homo sapiens
81 ERLEC1 27248
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
82 STIM1 6786
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 FKBP8 23770
Proximity Label-MS Homo sapiens
84 SLC26A5  
Two-hybrid Homo sapiens
85 FAM209A 200232
Two-hybrid Homo sapiens
86 ATP2A1 487
Proximity Label-MS Homo sapiens
87 RAB9A 9367
Proximity Label-MS Homo sapiens
88 TRIM27  
Two-hybrid Homo sapiens
89 LYVE1  
Two-hybrid Homo sapiens
90 GXYLT1 283464
Affinity Capture-MS Homo sapiens
91 RPN1 6184
Proximity Label-MS Homo sapiens
92 RNF2  
Two-hybrid Homo sapiens
93 STX4 6810
Proximity Label-MS Homo sapiens
94 CYP2C9  
Proximity Label-MS Homo sapiens
95 LPPR2  
Two-hybrid Homo sapiens
96 RNF185  
Affinity Capture-Western Homo sapiens
97 HLA-A 3105
Affinity Capture-Western Homo sapiens
98 LMNA 4000
Proximity Label-MS Homo sapiens
99 HIATL1 84641
Two-hybrid Homo sapiens
100 TRHR  
Two-hybrid Homo sapiens
101 ACBD5 91452
Proximity Label-MS Homo sapiens
102 B4GALT3 8703
Affinity Capture-MS Homo sapiens
103 GHITM 27069
Affinity Capture-MS Homo sapiens
104 MAPKAPK2 9261
Biochemical Activity Homo sapiens
105 TMEM51 55092
Two-hybrid Homo sapiens
106 FASN 2194
Negative Genetic Homo sapiens
107 GOLT1A  
Two-hybrid Homo sapiens
108 CDS1  
Two-hybrid Homo sapiens
109 EDEM1  
Affinity Capture-MS Homo sapiens
110 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
111 PJA2  
Two-hybrid Homo sapiens
112 EBP  
Two-hybrid Homo sapiens
113 MS4A6E  
Two-hybrid Homo sapiens
114 ERGIC1 57222
Proximity Label-MS Homo sapiens
115 AUP1 550
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
116 ERGIC2 51290
Proximity Label-MS Homo sapiens
117 Trim63  
Two-hybrid Rattus norvegicus
PCA Rattus norvegicus
118 SEC62 7095
Proximity Label-MS Homo sapiens
119 RAB7A 7879
Proximity Label-MS Homo sapiens
120 TOPORS  
Two-hybrid Homo sapiens
121 DNAJC1 64215
Proximity Label-MS Homo sapiens
122 KRAS 3845
Synthetic Lethality Homo sapiens
123 RAB5C 5878
Proximity Label-MS Homo sapiens
124 TMEM31  
Two-hybrid Homo sapiens
125 CKAP4 10970
Proximity Label-MS Homo sapiens
126 S1PR4  
Affinity Capture-MS Homo sapiens
127 ELOVL4  
Two-hybrid Homo sapiens
128 FCER1G 2207
Two-hybrid Homo sapiens
129 FAM210B  
Two-hybrid Homo sapiens
130 DERL1 79139
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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