Gene description for TMEM27
Gene name transmembrane protein 27
Gene symbol TMEM27
Other names/aliases NX-17
NX17
Species Homo sapiens
 Database cross references - TMEM27
ExoCarta ExoCarta_57393
Vesiclepedia VP_57393
Entrez Gene 57393
HGNC 29437
MIM 300631
UniProt Q9HBJ8  
 TMEM27 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for TMEM27
Molecular Function
    metallopeptidase activity GO:0008237 IEA
    protein binding GO:0005515 IPI
    peptidyl-dipeptidase activity GO:0008241 IEA
Biological Process
    proteolysis GO:0006508 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IDA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IDA
    positive regulation of amino acid transport GO:0051957 IEA
    positive regulation of SNARE complex assembly GO:0035543 IDA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    cytoplasm GO:0005737 IDA
    brush border membrane GO:0031526 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified TMEM27 in exosomes
1
Experiment ID 211
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
EV Biophysical techniques
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
MISEV standards
EV Biophysical techniques
AQP2
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for TMEM27
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TMEM27 is involved
No pathways found





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