Gene description for LMNA
Gene name lamin A/C
Gene symbol LMNA
Other names/aliases CDCD1
CDDC
CMD1A
CMT2B1
EMD2
FPL
FPLD
FPLD2
HGPS
IDC
LDP1
LFP
LGMD1B
LMN1
LMNC
LMNL1
PRO1
Species Homo sapiens
 Database cross references - LMNA
ExoCarta ExoCarta_4000
Entrez Gene 4000
HGNC 6636
MIM 150330
UniProt P02545  
 LMNA identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for LMNA
Molecular Function
    structural molecule activity GO:0005198 TAS
    protein binding GO:0005515 IPI
Biological Process
    regulation of protein localization to nucleus GO:1900180 IEA
    programmed cell death GO:0012501 TAS
    ventricular cardiac muscle cell development GO:0055015 IEA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IEA
    regulation of cell migration GO:0030334 ISS
    muscle organ development GO:0007517 IMP
    apoptotic process GO:0006915 TAS
    IRE1-mediated unfolded protein response GO:0036498 TAS
    cellular response to hypoxia GO:0071456 IEP
    mitotic nuclear envelope reassembly GO:0007084 TAS
    mitotic nuclear envelope disassembly GO:0007077 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    protein localization to nucleus GO:0034504 ISS
    positive regulation of cell aging GO:0090343 IDA
    sterol regulatory element binding protein import into nucleus GO:0035105 IEA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
    cellular protein metabolic process GO:0044267 TAS
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 ISS
Subcellular Localization
    lamin filament GO:0005638 TAS
    nuclear membrane GO:0031965 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nuclear envelope GO:0005635 TAS
    nuclear lamina GO:0005652 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    nuclear speck GO:0016607 IEA
    nucleoplasm GO:0005654 TAS
    intermediate filament GO:0005882 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified LMNA in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LMNA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RB1 5925
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 HTATIP  
Two-hybrid Homo sapiens
3 SREBF1 6720
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 TMPO 7112
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Far Western Homo sapiens
5 EMD  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
6 ALOX12 239
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 NARF 26502
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 LMNA 4000
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ZNF239  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 PRKCA 5578
Invivo Homo sapiens
11 TOR1AIP1 26092
Invitro Homo sapiens
12 LMNB1 4001
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 SYNE1 23345
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which LMNA is involved
PathwayEvidenceSource
XBP1(S) activates chaperone genes TAS Reactome





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