Gene description for BUD31
Gene name BUD31 homolog (S. cerevisiae)
Gene symbol BUD31
Other names/aliases Cwc14
EDG-2
EDG2
G10
YCR063W
fSAP17
Species Homo sapiens
 Database cross references - BUD31
ExoCarta ExoCarta_8896
Vesiclepedia VP_8896
Entrez Gene 8896
HGNC 29629
MIM 603477
UniProt P41223  
 BUD31 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for BUD31
Molecular Function
    protein binding GO:0005515 IPI
    nuclear receptor binding GO:0016922 IDA
    nuclear receptor coactivator activity GO:0030374 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of androgen receptor activity GO:2000825 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
 Experiment description of studies that identified BUD31 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BUD31
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC12  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 HNRNPC 3183
Affinity Capture-MS Homo sapiens
4 CCT3 7203
Two-hybrid Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 PRPF8 10594
Proximity Label-MS Homo sapiens
7 PPIE 10450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CRK 1398
Co-fractionation Homo sapiens
9 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
10 ID1  
Affinity Capture-MS Homo sapiens
11 CTNNBL1 56259
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 Ccdc12  
Affinity Capture-MS Mus musculus
13 TRIM55  
Co-fractionation Homo sapiens
14 SYF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LMNA 4000
Proximity Label-MS Homo sapiens
16 APP 351
Reconstituted Complex Homo sapiens
17 CENPA  
Proximity Label-MS Homo sapiens
18 USB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KIAA1429 25962
Affinity Capture-MS Homo sapiens
20 ISY1 57461
Co-fractionation Homo sapiens
21 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SF3A2 8175
Co-fractionation Homo sapiens
23 TRA2A 29896
Co-fractionation Homo sapiens
24 KRTAP10-3  
Two-hybrid Homo sapiens
25 KLHL20  
Affinity Capture-MS Homo sapiens
26 MECP2 4204
Affinity Capture-MS Homo sapiens
27 MYCN  
Affinity Capture-MS Homo sapiens
28 KRTAP10-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 LSM2 57819
Co-fractionation Homo sapiens
30 VTN 7448
Co-fractionation Homo sapiens
31 SCAF1  
Two-hybrid Homo sapiens
32 UTP14A 10813
Co-fractionation Homo sapiens
33 Klc4  
Two-hybrid Mus musculus
34 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
35 NPC1 4864
Two-hybrid Homo sapiens
36 CUL3 8452
Affinity Capture-MS Homo sapiens
37 PPIL1 51645
Affinity Capture-MS Homo sapiens
38 SRSF3 6428
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
39 RCC2 55920
Co-fractionation Homo sapiens
40 CBX3 11335
Proximity Label-MS Homo sapiens
41 RPL31 6160
Proximity Label-MS Homo sapiens
42 HTR5A  
Two-hybrid Homo sapiens
43 TPBG 7162
Co-fractionation Homo sapiens
44 MEOX2  
Two-hybrid Homo sapiens
45 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
47 CCDC101  
Co-fractionation Homo sapiens
48 SON 6651
Co-fractionation Homo sapiens
49 ADCY7 113
Two-hybrid Homo sapiens
50 CEBPA  
Protein-peptide Homo sapiens
51 CD3EAP  
Proximity Label-MS Homo sapiens
52 ZNF326 284695
Co-fractionation Homo sapiens
53 RBM39 9584
Affinity Capture-MS Homo sapiens
54 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PANX1 24145
Two-hybrid Homo sapiens
56 TPR 7175
Co-fractionation Homo sapiens
57 ERGIC3 51614
Two-hybrid Homo sapiens
58 SF1 7536
Two-hybrid Homo sapiens
59 NR3C1 2908
Proximity Label-MS Homo sapiens
60 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HOXA1 3198
Two-hybrid Homo sapiens
62 TSPAN7  
Two-hybrid Homo sapiens
63 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
64 KIAA1143  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PRPF19 27339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
66 ZC3H18  
Co-fractionation Homo sapiens
67 PABPC5 140886
Affinity Capture-MS Homo sapiens
68 TOP1 7150
Affinity Capture-MS Homo sapiens
69 BMI1  
Affinity Capture-MS Homo sapiens
70 SEPT7 989
Co-fractionation Homo sapiens
71 RNF208  
Two-hybrid Homo sapiens
72 HLA-A 3105
Two-hybrid Homo sapiens
73 KIAA0195 9772
Two-hybrid Homo sapiens
74 MARCH6  
Two-hybrid Homo sapiens
75 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
76 DNAH6 1768
Cross-Linking-MS (XL-MS) Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 BCAS2 10286
Affinity Capture-MS Homo sapiens
79 RRBP1 6238
Co-fractionation Homo sapiens
80 AKIRIN2  
Affinity Capture-MS Homo sapiens
81 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
82 SNRPA 6626
Affinity Capture-MS Homo sapiens
83 NUP50 10762
Proximity Label-MS Homo sapiens
84 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 RPS24 6229
Proximity Label-MS Homo sapiens
86 SRSF7 6432
Co-fractionation Homo sapiens
87 RELB  
Two-hybrid Homo sapiens
88 RPS7 6201
Co-fractionation Homo sapiens
89 RNASE1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 DCUN1D4  
Co-fractionation Homo sapiens
91 SNW1 22938
Co-fractionation Homo sapiens
92 KRAS 3845
Negative Genetic Homo sapiens
93 BEND5  
Two-hybrid Homo sapiens
94 MMP24 10893
Two-hybrid Homo sapiens
95 SNRPA1 6627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 ATP13A1 57130
Two-hybrid Homo sapiens
View the network image/svg+xml



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