Gene description for CRK
Gene name v-crk avian sarcoma virus CT10 oncogene homolog
Gene symbol CRK
Other names/aliases CRKII
p38
Species Homo sapiens
 Database cross references - CRK
ExoCarta ExoCarta_1398
Entrez Gene 1398
HGNC 2362
MIM 164762
UniProt P46108  
 CRK identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for CRK
Molecular Function
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 IPI
    SH3/SH2 adaptor activity GO:0005070 IEA
    protein phosphorylated amino acid binding GO:0045309 IEA
    ephrin receptor binding GO:0046875 IPI
Biological Process
    activation of MAPKK activity GO:0000186 TAS
    blood coagulation GO:0007596 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    regulation of actin cytoskeleton organization GO:0032956 IDA
    ephrin receptor signaling pathway GO:0048013 IDA
    platelet activation GO:0030168 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of GTPase activity GO:0043087 IDA
    innate immune response GO:0045087 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
Subcellular Localization
    plasma membrane GO:0005886 IEA
    actin cytoskeleton GO:0015629 IEA
    cytoplasm GO:0005737 IC
    cytosol GO:0005829 TAS
    nucleus GO:0005634 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CRK in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CRK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGAP17 55114
Reconstituted Complex Homo sapiens
2 DOCK1 1793
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 SH3BP1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 ABL2  
Invivo Homo sapiens
Invitro Homo sapiens
5 NCK1 4690
Reconstituted Complex Homo sapiens
6 PDGFRA  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 EPHA3 2042
Affinity Capture-Western Homo sapiens
8 PXN  
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
9 RICS  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 BUB1  
Two-hybrid Homo sapiens
11 XPO1 7514
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
12 ASAP1 50807
Reconstituted Complex Homo sapiens
13 NEDD9 4739
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
14 SOCS1  
Reconstituted Complex Homo sapiens
15 BCAR1 9564
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 SHB  
Invivo Homo sapiens
Invitro Homo sapiens
17 EPS15 2060
Invitro Homo sapiens
18 ERBB4 2066
Affinity Capture-MS Homo sapiens
19 PLSCR1 5359
Two-hybrid Homo sapiens
20 EGFR 1956
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
21 PTK2 5747
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 PDGFRB 5159
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 WEE1 7465
Invivo Homo sapiens
24 GAB1  
Invitro Homo sapiens
25 DPPA4  
Two-hybrid Homo sapiens
26 MAPK8 5599
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 RET 5979
Reconstituted Complex Homo sapiens
28 PTK2B 2185
Invivo Homo sapiens
Invitro Homo sapiens
29 ZAP70 7535
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
30 AVIL  
Reconstituted Complex Homo sapiens
31 SRC 6714
Affinity Capture-Western Homo sapiens
32 SNIP  
Affinity Capture-Western Homo sapiens
33 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 SYN1  
Invivo Homo sapiens
35 PSMC6 5706
Two-hybrid Homo sapiens
36 FRS2  
Reconstituted Complex Homo sapiens
37 RAPGEF1 2889
Invivo Homo sapiens
38 IRS4 8471
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 SOS1  
Invivo Homo sapiens
Invitro Homo sapiens
40 EPHB3 2049
Reconstituted Complex Homo sapiens
41 GRB2 2885
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
42 DOCK3  
Invitro Homo sapiens
Two-hybrid Homo sapiens
43 MAP4K1  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
44 MAP4K5  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
45 KHDRBS1 10657
Reconstituted Complex Homo sapiens
46 CBLC  
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
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