Gene description for CBLB
Gene name Cbl proto-oncogene B, E3 ubiquitin protein ligase
Gene symbol CBLB
Other names/aliases Cbl-b
Nbla00127
RNF56
Species Homo sapiens
 Database cross references - CBLB
ExoCarta ExoCarta_868
Vesiclepedia VP_868
Entrez Gene 868
HGNC 1542
MIM 604491
UniProt Q13191  
 CBLB identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CBLB
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    signal transducer activity GO:0004871 IEA
    ubiquitin-protein transferase activity GO:0004842 IEA
    ligase activity GO:0016874 IEA
    phosphotyrosine binding GO:0001784 IEA
Biological Process
    negative regulation of T cell receptor signaling pathway GO:0050860 IEA
    cell surface receptor signaling pathway GO:0007166 IEA
    negative regulation of alpha-beta T cell proliferation GO:0046642 IEA
    NLS-bearing protein import into nucleus GO:0006607 TAS
    intracellular signal transduction GO:0035556 IEA
    protein ubiquitination GO:0016567 IEA
    signal transduction GO:0007165 TAS
    positive regulation of T cell anergy GO:0002669 IEA
    immune response GO:0006955 IEA
    T cell activation GO:0042110 IEA
    positive regulation of protein catabolic process GO:0045732 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CBLB in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CBLB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLCG2 5336
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 NCK2 8440
Two-hybrid Homo sapiens
3 BLNK  
Affinity Capture-MS Homo sapiens
4 SH3KBP1 30011
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 BTK 695
Affinity Capture-Western Homo sapiens
6 VAV1 7409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 CRY1  
Two-hybrid Homo sapiens
8 NCK1 4690
Affinity Capture-MS Homo sapiens
9 RNF11 26994
Affinity Capture-MS Homo sapiens
10 NEDD4 4734
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 GRB2 2885
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 SHC1 6464
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 PIK3R1 5295
Affinity Capture-Western Homo sapiens
15 CRKL 1399
Invivo Homo sapiens
Invitro Homo sapiens
16 SORBS3 10174
Two-hybrid Homo sapiens
17 GORASP2 26003
Two-hybrid Homo sapiens
18 ZAP70 7535
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 SYK 6850
Affinity Capture-MS Homo sapiens
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