Gene description for CBLB
Gene name Cbl proto-oncogene B, E3 ubiquitin protein ligase
Gene symbol CBLB
Other names/aliases Cbl-b
Nbla00127
RNF56
Species Homo sapiens
 Database cross references - CBLB
ExoCarta ExoCarta_868
Vesiclepedia VP_868
Entrez Gene 868
HGNC 1542
MIM 604491
UniProt Q13191  
 CBLB identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CBLB
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 TAS
    receptor tyrosine kinase binding GO:0030971 IBA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    positive regulation of T cell anergy GO:0002669 IEA
    T cell anergy GO:0002870 IEA
    NLS-bearing protein import into nucleus GO:0006607 TAS
    immune response GO:0006955 IEA
    signal transduction GO:0007165 IBA
    protein ubiquitination GO:0016567 IEA
    protein catabolic process GO:0030163 IEA
    positive regulation of protein ubiquitination GO:0031398 IEA
    intracellular signal transduction GO:0035556 IEA
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IBA
    positive regulation of protein catabolic process GO:0045732 IEA
    T cell receptor signaling pathway GO:0050852 IEA
    negative regulation of T cell receptor signaling pathway GO:0050860 IEA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    regulation protein catabolic process at postsynapse GO:0140252 IEA
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IEA
    regulation of platelet-derived growth factor receptor-alpha signaling pathway GO:2000583 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    membrane raft GO:0045121 IBA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified CBLB in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CBLB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGEF7 8874
Co-crystal Structure Homo sapiens
2 SYK 6850
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
3 LCK 3932
Affinity Capture-Western Homo sapiens
4 TANK 10010
Affinity Capture-Western Homo sapiens
5 CBLB 868
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
6 VAV2 7410
Two-hybrid Homo sapiens
7 CFLAR  
Affinity Capture-Western Homo sapiens
8 GRB2 2885
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
9 SHC1 6464
Affinity Capture-Western Homo sapiens
10 NEDD4 4734
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 RNF11 26994
Two-hybrid Homo sapiens
12 SIRT2 22933
Affinity Capture-Western Homo sapiens
13 MYD88 4615
Affinity Capture-Western Homo sapiens
14 VAV3 10451
Two-hybrid Homo sapiens
15 DDR2 4921
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 GORASP2 26003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 CRK 1398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CRY1  
Two-hybrid Homo sapiens
19 SH3RF1  
Two-hybrid Homo sapiens
20 CD2AP 23607
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
21 VAV1 7409
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 TRAF6 7189
Affinity Capture-Western Homo sapiens
23 DUXAP9  
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
24 CARD11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 ABL1 25
Affinity Capture-MS Homo sapiens
26 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
27 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
28 SORBS2  
Two-hybrid Homo sapiens
29 MS4A2  
Co-fractionation Homo sapiens
30 PTK2 5747
Affinity Capture-Western Homo sapiens
31 PDGFRB 5159
Affinity Capture-Western Homo sapiens
32 UBE2D2 7322
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
33 TRAF3 7187
Affinity Capture-Western Homo sapiens
34 HNRNPK 3190
Reconstituted Complex Homo sapiens
35 Cep152  
Affinity Capture-MS Mus musculus
36 NCK2 8440
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 PTPN11 5781
Affinity Capture-Western Homo sapiens
38 CBL 867
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 PTK6  
Two-hybrid Homo sapiens
40 WAS 7454
Affinity Capture-Western Homo sapiens
41 IPO8 10526
Affinity Capture-MS Homo sapiens
42 ITSN2 50618
Affinity Capture-MS Homo sapiens
43 SORBS3 10174
Two-hybrid Homo sapiens
44 SP7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 PLCG2 5336
Affinity Capture-Western Homo sapiens
46 UBASH3B 84959
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 UBASH3A 53347
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
48 USP12 219333
Proximity Label-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 CRKL 1399
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 UBE2L3 7332
Reconstituted Complex Homo sapiens
52 SPRY1 10252
Affinity Capture-Western Homo sapiens
53 PTK2B 2185
Affinity Capture-Western Homo sapiens
54 ZAP70 7535
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 OSTF1 26578
Two-hybrid Homo sapiens
56 YES1 7525
Two-hybrid Homo sapiens
57 EPHA2 1969
Proximity Label-MS Homo sapiens
58 TGFB1I1 7041
Two-hybrid Homo sapiens
59 GLRX3 10539
Two-hybrid Homo sapiens
60 PIK3R2 5296
Affinity Capture-Western Homo sapiens
61 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 BTK 695
Affinity Capture-Western Homo sapiens
63 ITSN1 6453
Affinity Capture-Western Homo sapiens
64 OBSL1 23363
Affinity Capture-MS Homo sapiens
65 ASAP1 50807
Affinity Capture-Western Homo sapiens
66 IRS1 3667
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 SH3KBP1 30011
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
68 CYLD  
Affinity Capture-Western Homo sapiens
69 NEDD8 4738
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
70 TICAM1  
Affinity Capture-Western Homo sapiens
71 KIT 3815
Affinity Capture-Western Homo sapiens
72 DSCR3 10311
Two-hybrid Homo sapiens
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