Gene description for GORASP2
Gene name golgi reassembly stacking protein 2, 55kDa
Gene symbol GORASP2
Other names/aliases GOLPH6
GRASP55
GRS2
p59
Species Homo sapiens
 Database cross references - GORASP2
ExoCarta ExoCarta_26003
Vesiclepedia VP_26003
Entrez Gene 26003
HGNC 17500
MIM 608693
UniProt Q9H8Y8  
 GORASP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for GORASP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    organelle organization GO:0006996 IMP
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 NAS
    spermatogenesis GO:0007283 IEA
    cell differentiation GO:0030154 IEA
    response to endoplasmic reticulum stress GO:0034976 IDA
    establishment of protein localization to plasma membrane GO:0061951 IDA
    establishment of protein localization to plasma membrane GO:0061951 IMP
    organelle assembly GO:0070925 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 NAS
    membrane GO:0016020 TAS
 Experiment description of studies that identified GORASP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GORASP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 GSTP1 2950
Affinity Capture-MS Homo sapiens
3 SCFD1 23256
Affinity Capture-MS Homo sapiens
4 GSTZ1 2954
Two-hybrid Homo sapiens
5 ZMYND19  
Two-hybrid Homo sapiens
6 GJD3  
Proximity Label-MS Homo sapiens
7 DECR2 26063
Affinity Capture-MS Homo sapiens
8 ZMAT1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 MORN2  
Two-hybrid Homo sapiens
10 ETHE1 23474
Two-hybrid Homo sapiens
11 OCLN 100506658
Proximity Label-MS Homo sapiens
12 PSMD6 9861
Two-hybrid Homo sapiens
13 MARCKS 4082
Proximity Label-MS Homo sapiens
14 TRAF4 9618
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 MYOM3  
Two-hybrid Homo sapiens
16 KIFC3 3801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 C3orf58 205428
Affinity Capture-MS Homo sapiens
18 AES 166
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 TMED4 222068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NUP62 23636
Two-hybrid Homo sapiens
21 ENOX2  
Two-hybrid Homo sapiens
22 UBA1 7317
Co-fractionation Homo sapiens
23 NMNAT1  
Two-hybrid Homo sapiens
24 ISCA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EMD 2010
Proximity Label-MS Homo sapiens
26 HSD17B11 51170
Proximity Label-MS Homo sapiens
27 LAMP2 3920
Proximity Label-MS Homo sapiens
28 HNRNPR 10236
Co-fractionation Homo sapiens
29 UBL3 5412
Two-hybrid Homo sapiens
30 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 GNGT1  
Two-hybrid Homo sapiens
32 STX4 6810
Proximity Label-MS Homo sapiens
33 BET1 10282
Affinity Capture-MS Homo sapiens
34 SBDS 51119
Co-fractionation Homo sapiens
35 TEKT3 64518
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 CCDC90B  
Two-hybrid Homo sapiens
37 RAB3B 5865
Proximity Label-MS Homo sapiens
38 UGDH 7358
Co-fractionation Homo sapiens
39 GOLGA6L2  
Two-hybrid Homo sapiens
40 HSBP1 3281
Two-hybrid Homo sapiens
41 TP53RK 112858
Two-hybrid Homo sapiens
42 B3GAT1  
Proximity Label-MS Homo sapiens
43 TSC22D4 81628
Two-hybrid Homo sapiens
44 PRDX3 10935
Two-hybrid Homo sapiens
45 DPYSL2 1808
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 STOX1  
Two-hybrid Homo sapiens
47 GABPB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 CARHSP1 23589
Two-hybrid Homo sapiens
49 PCBD1 5092
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 NHSL2  
Two-hybrid Homo sapiens
51 ZNF148  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 TMED2 10959
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NCOA5  
Two-hybrid Homo sapiens
54 MMP15 4324
Affinity Capture-MS Homo sapiens
55 TMEM165 55858
Affinity Capture-MS Homo sapiens
56 CNTNAP2  
Two-hybrid Homo sapiens
57 MAPK14 1432
Biochemical Activity Homo sapiens
58 SSR1 6745
Proximity Label-MS Homo sapiens
59 EWSR1 2130
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
60 PRPS1 5631
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 RHOA 387
Co-fractionation Homo sapiens
62 KCTD7 154881
Two-hybrid Homo sapiens
63 ARF4 378
Proximity Label-MS Homo sapiens
64 DCTD 1635
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 Blzf1 66352
Affinity Capture-MS Mus musculus
66 MID2 11043
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 RAB1A 5861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 STX6 10228
Proximity Label-MS Homo sapiens
69 SLC30A5 64924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 FAM98B 283742
Co-fractionation Homo sapiens
71 APIP 51074
Two-hybrid Homo sapiens
72 ENOX1  
Two-hybrid Homo sapiens
73 FIG4 9896
Two-hybrid Homo sapiens
74 ZBTB14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 LMAN2 10960
Affinity Capture-MS Homo sapiens
76 PRCC  
Affinity Capture-MS Homo sapiens
77 RBM46  
Two-hybrid Homo sapiens
78 RAB35 11021
Proximity Label-MS Homo sapiens
79 CBLB 868
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 PRKAB2 5565
Two-hybrid Homo sapiens
81 TSN 7247
Two-hybrid Homo sapiens
82 EBAG9 9166
Proximity Label-MS Homo sapiens
83 ARHGEF15  
Two-hybrid Homo sapiens
84 ATP6AP1 537
Affinity Capture-MS Homo sapiens
85 EEF1A1 1915
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
86 KCTD13 253980
Two-hybrid Homo sapiens
87 METTL7A 25840
Proximity Label-MS Homo sapiens
88 LAMTOR1 55004
Proximity Label-MS Homo sapiens
89 ATP2C1 27032
Affinity Capture-MS Homo sapiens
90 RER1 11079
Affinity Capture-MS Homo sapiens
91 RAB1B 81876
Affinity Capture-MS Homo sapiens
92 LAMP3  
Proximity Label-MS Homo sapiens
93 SEC22A  
Affinity Capture-MS Homo sapiens
94 NUP155 9631
Proximity Label-MS Homo sapiens
95 MAPK1 5594
Biochemical Activity Homo sapiens
96 PXMP2  
Proximity Label-MS Homo sapiens
97 E2F4  
Affinity Capture-MS Homo sapiens
98 LRRC59 55379
Proximity Label-MS Homo sapiens
99 LONRF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 ZDHHC3 51304
Affinity Capture-MS Homo sapiens
101 REEP5 7905
Proximity Label-MS Homo sapiens
102 PANX1 24145
Proximity Label-MS Homo sapiens
103 PPIB 5479
Co-fractionation Homo sapiens
104 MIF 4282
Two-hybrid Homo sapiens
105 GJA1 2697
Proximity Label-MS Homo sapiens
106 ODC1 4953
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
107 CDC23 8697
Two-hybrid Homo sapiens
108 ATP6V1G1 9550
Two-hybrid Homo sapiens
109 INPP4B 8821
Co-fractionation Homo sapiens
110 PARK2  
Affinity Capture-MS Homo sapiens
111 CDK5 1020
Affinity Capture-MS Homo sapiens
112 ALKBH4  
Two-hybrid Homo sapiens
113 CXADR 1525
Proximity Label-MS Homo sapiens
114 SHMT1 6470
Two-hybrid Homo sapiens
115 MSC  
Two-hybrid Homo sapiens
116 GNG7 2788
Two-hybrid Homo sapiens
117 NONO 4841
Co-fractionation Homo sapiens
118 SCAND1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
119 MCAM 4162
Proximity Label-MS Homo sapiens
120 GOLGA2 2801
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 PNMA5 114824
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
122 HOXC5  
Two-hybrid Homo sapiens
123 TAGLN 6876
Two-hybrid Homo sapiens
124 EMILIN3  
Two-hybrid Homo sapiens
125 RGS3 5998
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
126 CDH1 999
Proximity Label-MS Homo sapiens
127 ZBED1  
Two-hybrid Homo sapiens
128 OGT 8473
Reconstituted Complex Homo sapiens
129 AHNAK 79026
Co-fractionation Homo sapiens
130 JADE2 23338
Two-hybrid Homo sapiens
131 LAMP1 3916
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 CCDC153  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 RAB2A 5862
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 ACY3 91703
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
135 RPN1 6184
Proximity Label-MS Homo sapiens
136 AAMDC  
Two-hybrid Homo sapiens
137 STX5 6811
Affinity Capture-MS Homo sapiens
138 TECR 9524
Co-fractionation Homo sapiens
139 CEP76  
Two-hybrid Homo sapiens
140 MAPK8 5599
Biochemical Activity Homo sapiens
141 FLOT2 2319
Affinity Capture-MS Homo sapiens
142 NQO2 4835
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
143 ELOVL5 60481
Proximity Label-MS Homo sapiens
144 SEC61B 10952
Proximity Label-MS Homo sapiens
145 TMED1 11018
Affinity Capture-MS Homo sapiens
146 MAN1A1 4121
Affinity Capture-MS Homo sapiens
147 RMDN3 55177
Co-fractionation Homo sapiens
148 ACBD3 64746
Co-fractionation Homo sapiens
149 GTF2A1 2957
Co-fractionation Homo sapiens
150 BAG3 9531
Co-fractionation Homo sapiens
151 SLC35E1 79939
Affinity Capture-MS Homo sapiens
152 CGGBP1 8545
Two-hybrid Homo sapiens
153 TXLNA 200081
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
154 HSPA5 3309
Affinity Capture-MS Homo sapiens
155 PSMA3 5684
Two-hybrid Homo sapiens
156 SEPT14  
Two-hybrid Homo sapiens
157 BTBD3  
Two-hybrid Homo sapiens
158 TMED9 54732
Affinity Capture-MS Homo sapiens
159 RPN2 6185
Proximity Label-MS Homo sapiens
160 CANX 821
Affinity Capture-MS Homo sapiens
161 TMED3 23423
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
162 XPO1 7514
Affinity Capture-MS Homo sapiens
163 KCTD9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
164 UBAP2 55833
Affinity Capture-MS Homo sapiens
165 MATN4 8785
Two-hybrid Homo sapiens
166 CRYAB 1410
Two-hybrid Homo sapiens
167 CRYAA 1409
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
168 UBE2Z 65264
Two-hybrid Homo sapiens
169 SLC30A6 55676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 BCAP31 10134
Proximity Label-MS Homo sapiens
171 HSD3B7 80270
Proximity Label-MS Homo sapiens
172 CUL3 8452
Co-fractionation Homo sapiens
173 STIM1 6786
Proximity Label-MS Homo sapiens
174 LTBP3 4054
Two-hybrid Homo sapiens
175 ELAC2 60528
Co-fractionation Homo sapiens
176 ARF6 382
Proximity Label-MS Homo sapiens
177 TSEN15 116461
Two-hybrid Homo sapiens
178 SFT2D3  
Affinity Capture-MS Homo sapiens
179 LAMTOR5 10542
Co-fractionation Homo sapiens
180 SCYL2 55681
Affinity Capture-MS Homo sapiens
181 MIEN1 84299
Two-hybrid Homo sapiens
182 SEPT1 1731
Two-hybrid Homo sapiens
183 KRTAP5-11  
Two-hybrid Homo sapiens
184 NAPA 8775
Affinity Capture-MS Homo sapiens
185 TRPT1 83707
Two-hybrid Homo sapiens
186 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
187 Uso1 56041
Affinity Capture-MS Mus musculus
188 NME1 4830
Two-hybrid Homo sapiens
189 ATP2A1 487
Proximity Label-MS Homo sapiens
190 SELENBP1 8991
Two-hybrid Homo sapiens
191 RAD54B  
Two-hybrid Homo sapiens
192 RAB9A 9367
Proximity Label-MS Homo sapiens
193 TRAF5  
Two-hybrid Homo sapiens
194 BLMH 642
Co-fractionation Homo sapiens
195 C1orf94  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
196 EEF1A2 1917
Co-fractionation