Gene description for APIP
Gene name APAF1 interacting protein
Gene symbol APIP
Other names/aliases APIP2
CGI-29
CGI29
MMRP19
hAPIP
Species Homo sapiens
 Database cross references - APIP
ExoCarta ExoCarta_51074
Vesiclepedia VP_51074
Entrez Gene 51074
HGNC 17581
MIM 612491
UniProt Q96GX9  
 APIP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for APIP
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    identical protein binding GO:0042802 IPI
    methylthioribulose 1-phosphate dehydratase activity GO:0046570 IBA
    methylthioribulose 1-phosphate dehydratase activity GO:0046570 IDA
Biological Process
    apoptotic process GO:0006915 IEA
    L-methionine salvage from methylthioadenosine GO:0019509 IBA
    L-methionine salvage from methylthioadenosine GO:0019509 IEA
    L-methionine salvage from methylthioadenosine GO:0019509 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    protein homotetramerization GO:0051289 IDA
    pyroptotic inflammatory response GO:0070269 IMP
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified APIP in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for APIP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ITGA5 3678
Affinity Capture-MS Homo sapiens
3 LYG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SDCBP 6386
Two-hybrid Homo sapiens
5 RNASE7 84659
Affinity Capture-MS Homo sapiens
6 KPRP 448834
Affinity Capture-MS Homo sapiens
7 PDZD4  
Two-hybrid Homo sapiens
8 SERPINA12  
Affinity Capture-MS Homo sapiens
9 HEPHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TGM3 7053
Affinity Capture-MS Homo sapiens
11 SDR9C7  
Affinity Capture-MS Homo sapiens
12 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Emc6  
Affinity Capture-MS Mus musculus
14 POF1B 79983
Affinity Capture-MS Homo sapiens
15 SKP1 6500
Two-hybrid Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 BAZ2B  
Two-hybrid Homo sapiens
18 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
20 KLK7 5650
Affinity Capture-MS Homo sapiens
21 NUDT18  
Two-hybrid Homo sapiens
22 ALOX12B  
Affinity Capture-MS Homo sapiens
23 PSMA1 5682
Two-hybrid Homo sapiens
24 PPP1CC 5501
Two-hybrid Homo sapiens
25 NDE1 54820
Affinity Capture-MS Homo sapiens
26 TSC22D4 81628
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
27 FASTKD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TNPO2 30000
Two-hybrid Homo sapiens
29 Dctn3  
Affinity Capture-MS Mus musculus
30 WDYHV1  
Two-hybrid Homo sapiens
31 APAF1 317
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
32 GORASP2 26003
Two-hybrid Homo sapiens
33 ZBTB25  
Two-hybrid Homo sapiens
34 IMPDH2 3615
Two-hybrid Homo sapiens
35 UBA3 9039
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SBSN 374897
Affinity Capture-MS Homo sapiens
37 TGM1 7051
Affinity Capture-MS Homo sapiens
38 ARMT1 79624
Co-fractionation Homo sapiens
39 IL37  
Affinity Capture-MS Homo sapiens
40 VIM 7431
Two-hybrid Homo sapiens
41 ARL4C 10123
Affinity Capture-MS Homo sapiens
42 LNX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 DSG4 147409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GSDMA 284110
Affinity Capture-MS Homo sapiens
45 C6orf165  
Two-hybrid Homo sapiens
46 VPS25 84313
Two-hybrid Homo sapiens
47 APIP 51074
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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