Gene description for SKP1
Gene name S-phase kinase-associated protein 1
Gene symbol SKP1
Other names/aliases EMC19
OCP-II
OCP2
SKP1A
TCEB1L
p19A
Species Homo sapiens
 Database cross references - SKP1
ExoCarta ExoCarta_6500
Entrez Gene 6500
HGNC 10899
MIM 601434
UniProt P63208  
 SKP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SKP1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IDA
    mitotic cell cycle GO:0000278 TAS
    circadian rhythm GO:0007623 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    protein ubiquitination GO:0016567 IDA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    innate immune response GO:0045087 TAS
    histone H2A monoubiquitination GO:0035518 IDA
    viral process GO:0016032 TAS
    Notch signaling pathway GO:0007219 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IDA
    PcG protein complex GO:0031519 IDA
    extracellular exosome GO:0070062 IDA
    nucleoplasm GO:0005654 TAS
    Cul7-RING ubiquitin ligase complex GO:0031467 IDA
 Experiment description of studies that identified SKP1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SKP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-MS Homo sapiens
2 CUL7  
Affinity Capture-Western Homo sapiens
3 FBXO7  
Affinity Capture-MS Homo sapiens
4 FBXL4 26235
Reconstituted Complex Homo sapiens
5 SUGT1 10910
Reconstituted Complex Homo sapiens
6 FBXW2  
Affinity Capture-MS Homo sapiens
7 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 BTRC 8945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 FBXW11  
Affinity Capture-Western Homo sapiens
10 CCNA2 890
Invivo Homo sapiens
11 FBXO32  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
12 CDCA3 83461
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 FBXL5  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 FBXL2  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 CTNNB1 1499
Affinity Capture-Western Homo sapiens
16 FBXO4  
Affinity Capture-MS Homo sapiens
17 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SKP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
19 RBX1 9978
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 FBXO34  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 FBXL3  
Invivo Homo sapiens
Two-hybrid Homo sapiens
22 TBK1 29110
Affinity Capture-MS Homo sapiens
23 FBXO2 26232
Invivo Homo sapiens
24 CDK9 1025
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 TBL1X 6907
Affinity Capture-Western Homo sapiens
26 FBXW7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 FBXW8 26259
Affinity Capture-Western Homo sapiens
28 FBXO18 84893
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
29 CACYBP 27101
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 FBXO5  
Affinity Capture-MS Homo sapiens
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