Gene description for TNFRSF10B
Gene name tumor necrosis factor receptor superfamily, member 10b
Gene symbol TNFRSF10B
Other names/aliases CD262
DR5
KILLER
KILLER/DR5
TRAIL-R2
TRAILR2
TRICK2
TRICK2A
TRICK2B
TRICKB
ZTNFR9
Species Homo sapiens
 Database cross references - TNFRSF10B
ExoCarta ExoCarta_8795
Entrez Gene 8795
HGNC 11905
MIM 603612
UniProt O14763  
 TNFRSF10B identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for TNFRSF10B
Molecular Function
    protein binding GO:0005515 IPI
    receptor activity GO:0004872 NAS
    TRAIL binding GO:0045569 NAS
Biological Process
    regulation of apoptotic process GO:0042981 NAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 NAS
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    necroptotic process GO:0070266 TAS
    response to endoplasmic reticulum stress GO:0034976 IDA
    regulation of necrotic cell death GO:0010939 TAS
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    programmed cell death GO:0012501 TAS
    apoptotic process GO:0006915 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 NAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    programmed necrotic cell death GO:0097300 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
Subcellular Localization
    integral component of membrane GO:0016021 IC
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    intracellular GO:0005622 TAS
 Experiment description of studies that identified TNFRSF10B in exosomes
1
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for TNFRSF10B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FASLG 356
Co-purification Homo sapiens
2 ARHGDIA 396
Co-purification Homo sapiens
3 EZR 7430
Co-purification Homo sapiens
4 CASP8  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
5 FADD 8772
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
6 TNFRSF1A 7132
Co-purification Homo sapiens
7 CFLAR  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 MSN 4478
Co-purification Homo sapiens
9 TRADD 8717
Affinity Capture-Western Homo sapiens
10 BID  
Co-purification Homo sapiens
11 RHOA 387
Co-purification Homo sapiens
12 MAPK8 5599
Co-purification Homo sapiens
13 TIMM50 92609
Two-hybrid Homo sapiens
14 TNFSF10 8743
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
15 FAS 355
Co-purification Homo sapiens
16 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
17 RIPK1  
Affinity Capture-Western Homo sapiens
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