Gene description for MARCH3
Gene name membrane-associated ring finger (C3HC4) 3, E3 ubiquitin protein ligase
Gene symbol MARCH3
Other names/aliases MARCH-III
RNF173
Species Homo sapiens
 Database cross references - MARCH3
ExoCarta ExoCarta_115123
Vesiclepedia VP_115123
Entrez Gene 115123
HGNC 28728
MIM 613333
UniProt Q86UD3  
 MARCH3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MARCH3
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    endocytosis GO:0006897 IEA
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    lysosome GO:0005764 IDA
    endosome GO:0005768 IDA
    early endosome membrane GO:0031901 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified MARCH3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MARCH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATAD1 84896
Affinity Capture-MS Homo sapiens
2 AASDHPPT 60496
Affinity Capture-MS Homo sapiens
3 PTPRF 5792
Affinity Capture-MS Homo sapiens
4 UBE2W  
Two-hybrid Homo sapiens
5 HMGCR  
Affinity Capture-MS Homo sapiens
6 SLC39A10 57181
Affinity Capture-MS Homo sapiens
7 NCSTN 23385
Affinity Capture-MS Homo sapiens
8 TFRC 7037
Affinity Capture-MS Homo sapiens
9 ENDOD1 23052
Affinity Capture-MS Homo sapiens
10 NOTCH2 4853
Affinity Capture-MS Homo sapiens
11 BAG2 9532
Affinity Capture-MS Homo sapiens
12 LSR 51599
Affinity Capture-MS Homo sapiens
13 UBE2M 9040
Affinity Capture-MS Homo sapiens
14 TM9SF2 9375
Affinity Capture-MS Homo sapiens
15 SRP9 6726
Affinity Capture-MS Homo sapiens
16 VAMP7 6845
Affinity Capture-MS Homo sapiens
17 RNFT1  
Affinity Capture-MS Homo sapiens
18 CBR1 873
Affinity Capture-MS Homo sapiens
19 UBE2E1  
Two-hybrid Homo sapiens
20 APLP2 334
Affinity Capture-MS Homo sapiens
21 UBE2D4  
Two-hybrid Homo sapiens
22 UBE2D2 7322
Two-hybrid Homo sapiens
23 IL3RA  
Affinity Capture-Western Homo sapiens
24 NDUFS2 4720
Affinity Capture-MS Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 CHPF2 54480
Affinity Capture-MS Homo sapiens
27 ATF6B  
Affinity Capture-MS Homo sapiens
28 WWP2 11060
Affinity Capture-MS Homo sapiens
29 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
30 APOL2 23780
Two-hybrid Homo sapiens
31 UBE2E2  
Two-hybrid Homo sapiens
32 DPYSL2 1808
Affinity Capture-MS Homo sapiens
33 UBE2K 3093
Two-hybrid Homo sapiens
34 NEDD4L 23327
Affinity Capture-MS Homo sapiens
35 UBE2D3 7323
Two-hybrid Homo sapiens
36 TMEM201 199953
Affinity Capture-MS Homo sapiens
37 FATE1  
Two-hybrid Homo sapiens
38 ITCH 83737
Affinity Capture-MS Homo sapiens
39 TYMS 7298
Affinity Capture-MS Homo sapiens
40 ILF3 3609
Affinity Capture-MS Homo sapiens
41 TMX2 51075
Affinity Capture-MS Homo sapiens
42 SLC3A2 6520
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MARCH3 is involved
No pathways found





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