ExoCarta: Gene summary

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Gene description for TYMS
Gene name	thymidylate synthetase
Gene symbol	TYMS
Other names/aliases	HST422 TMS TS
Species	Homo sapiens
Database cross references - TYMS
ExoCarta	ExoCarta_7298
Vesiclepedia	VP_7298
Entrez Gene	7298
HGNC	12441
MIM	188350
UniProt	P04818
TYMS identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells	36408942

Gene ontology annotations for TYMS

Molecular Function
	mRNA regulatory element binding translation repressor activity	GO:0000900	IDA
	thymidylate synthase activity	GO:0004799	IBA
	thymidylate synthase activity	GO:0004799	IDA
	thymidylate synthase activity	GO:0004799	TAS
	folic acid binding	GO:0005542	IC
	protein homodimerization activity	GO:0042803	IEA
	sequence-specific mRNA binding	GO:1990825	IDA
Biological Process
	dTMP biosynthetic process	GO:0006231	IBA
	dTMP biosynthetic process	GO:0006231	IDA
	dTTP biosynthetic process	GO:0006235	IEA
	circadian rhythm	GO:0007623	IEA
	response to xenobiotic stimulus	GO:0009410	IEA
	response to toxic substance	GO:0009636	IEA
	negative regulation of translation	GO:0017148	IDA
	uracil metabolic process	GO:0019860	IEA
	methylation	GO:0032259	IEA
	response to progesterone	GO:0032570	IEA
	response to vitamin A	GO:0033189	IEA
	response to cytokine	GO:0034097	IEA
	tetrahydrofolate interconversion	GO:0035999	IDA
	response to ethanol	GO:0045471	IEA
	response to organophosphorus	GO:0046683	IEP
	developmental growth	GO:0048589	IEA
	cartilage development	GO:0051216	IEA
	response to glucocorticoid	GO:0051384	IEA
	response to folic acid	GO:0051593	IEA
	intestinal epithelial cell maturation	GO:0060574	IEA
	DNA biosynthetic process	GO:0071897	IC
	liver regeneration	GO:0097421	IEA
Subcellular Localization
	nucleus	GO:0005634	IDA
	cytoplasm	GO:0005737	IDA
	mitochondrion	GO:0005739	IBA
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	IDA
	mitochondrial matrix	GO:0005759	IDA
	cytosol	GO:0005829	IBA
	cytosol	GO:0005829	TAS

Experiment description of studies that identified TYMS in exosomes

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for TYMS

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

IGBP1

3476

Co-fractionation

Homo sapiens

VCAM1

7412

Affinity Capture-MS

Homo sapiens

ZRANB2

9406

Co-fractionation

Homo sapiens

MARCH3

115123

Affinity Capture-MS

Homo sapiens

C9orf72

Affinity Capture-MS

Homo sapiens

WDR45B

56270

Co-fractionation

Homo sapiens

UBQLN4

56893

Co-fractionation

Homo sapiens

PLS3

5358

Co-fractionation

Homo sapiens

PKIG

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST1H3A

8350

Cross-Linking-MS (XL-MS)

Homo sapiens

DCK

1633

Co-fractionation

Homo sapiens

VPS4B

9525

Co-fractionation

Homo sapiens

BFAR

Affinity Capture-MS

Homo sapiens

TP53BP1

7158

Negative Genetic

Homo sapiens

TP53

7157

Negative Genetic

Homo sapiens

AHCYL1

10768

Co-fractionation

Homo sapiens

ADSL

158

Co-fractionation

Homo sapiens

WDR1

9948

Co-fractionation

Homo sapiens

UNK

Co-fractionation

Homo sapiens

UBXN7

26043

Co-fractionation

Homo sapiens

ATP6V1C1

528

Co-fractionation

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

NF2

4771

Negative Genetic

Homo sapiens

RTN1

6252

Affinity Capture-MS

Homo sapiens

PBRM1

55193

Negative Genetic

Homo sapiens

TPMT

7172

Co-fractionation

Homo sapiens

SSSCA1

10534

Co-fractionation

Homo sapiens

LIMS1

3987

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PDCD6

10016

Co-fractionation

Homo sapiens

PFDN1

5201

Affinity Capture-MS

Homo sapiens

LMNA

4000

Proximity Label-MS

Homo sapiens

PTEN

5728

Negative Genetic

Homo sapiens

ITGA4

3676

Affinity Capture-MS

Homo sapiens

UBE2C

11065

Co-fractionation

Homo sapiens

UBE2E1

Co-fractionation

Homo sapiens

DUT

1854

Co-fractionation

Homo sapiens

TXNDC17

84817

Co-fractionation

Homo sapiens

PPAT

5471

Co-fractionation

Homo sapiens

PPP4R1L

Affinity Capture-MS

Homo sapiens

ZFYVE19

84936

Co-fractionation

Homo sapiens

SBDS

51119

Co-fractionation

Homo sapiens

PSD4

Affinity Capture-MS

Homo sapiens

Tuba3a

22144

Affinity Capture-MS

Mus musculus

NF1

4763

Negative Genetic

Homo sapiens

UBE2M

9040

Co-fractionation

Homo sapiens

ARHGEF35

Affinity Capture-MS

Homo sapiens

TXN

7295

Co-fractionation

Homo sapiens

MARCH4

Affinity Capture-MS

Homo sapiens

EFHD2

79180

Affinity Capture-MS

Homo sapiens

PFDN2

5202

Affinity Capture-MS

Homo sapiens

SAE1

10055

Co-fractionation

Homo sapiens

PSMA5

5686

Co-fractionation

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

UBE2K

3093

Co-fractionation

Homo sapiens

EIF5A

1984

Co-fractionation

Homo sapiens

GOT1

2805

Co-fractionation

Homo sapiens

ZFYVE21

Co-fractionation

Homo sapiens

CHEK1

Negative Genetic

Homo sapiens

REXO2

25996

Co-fractionation

Homo sapiens

STK11

6794

Negative Genetic

Homo sapiens

SERBP1

26135

Affinity Capture-MS

Homo sapiens

STMN1

3925

Co-fractionation

Homo sapiens

NAPG

8774

Affinity Capture-MS

Homo sapiens

UBE2A

Co-fractionation

Homo sapiens

ATIC

471

Co-fractionation

Homo sapiens

PGAM1

5223

Co-fractionation

Homo sapiens

MAPK14

1432

Co-fractionation

Homo sapiens

RNF5

Affinity Capture-MS

Homo sapiens

TANC2

Affinity Capture-MS

Homo sapiens

UBE2B

7320

Co-fractionation

Homo sapiens

UPF2

26019

Two-hybrid

Homo sapiens

SH3GL2

Two-hybrid

Homo sapiens

ATP6V1E1

529

Co-fractionation

Homo sapiens

MARCH6

Affinity Capture-MS

Homo sapiens

PHGDH

26227

Co-fractionation

Homo sapiens

PROSER2

254427

Affinity Capture-MS

Homo sapiens

UFC1

51506

Co-fractionation

Homo sapiens

View the network

Pathways in which TYMS is involved

Pathway	Evidence	Source
Cell Cycle	TAS	Reactome
Cell Cycle, Mitotic	TAS	Reactome
G1/S Transition	TAS	Reactome
G1/S-Specific Transcription	TAS	Reactome
Interconversion of nucleotide di- and triphosphates	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of nucleotides	TAS	Reactome
Mitotic G1 phase and G1/S transition	TAS	Reactome

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