Gene description for TPMT
Gene name thiopurine S-methyltransferase
Gene symbol TPMT
Other names/aliases -
Species Homo sapiens
 Database cross references - TPMT
ExoCarta ExoCarta_7172
Entrez Gene 7172
HGNC 12014
MIM 187680
UniProt P51580  
 TPMT identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TPMT
Molecular Function
    thiopurine S-methyltransferase activity GO:0008119 IEA
Biological Process
    methylation GO:0032259 TAS
    small molecule metabolic process GO:0044281 TAS
    xenobiotic metabolic process GO:0006805 TAS
    nucleobase-containing compound metabolic process GO:0006139 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TPMT in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TPMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TPMT is involved
PathwayEvidenceSource
Defective TPMT causes Thiopurine S-methyltransferase deficiency (TPMT deficiency) TAS Reactome
Methylation TAS Reactome





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