Gene description for NOTCH2NL
Gene name notch 2 N-terminal like
Gene symbol NOTCH2NL
Other names/aliases N2N
Species Homo sapiens
 Database cross references - NOTCH2NL
ExoCarta ExoCarta_388677
Vesiclepedia VP_388677
Entrez Gene 388677
HGNC 31862
UniProt Q7Z3S9  
 NOTCH2NL identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for NOTCH2NL
Molecular Function
    Notch binding GO:0005112 IPI
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    Notch signaling pathway GO:0007219 IEA
    cerebral cortex development GO:0021987 IDA
    cell differentiation GO:0030154 IEA
    positive regulation of Notch signaling pathway GO:0045747 IDA
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified NOTCH2NL in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NOTCH2NL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LCE1F  
Two-hybrid Homo sapiens
2 RAB3IL1 5866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 PRR35  
Two-hybrid Homo sapiens
4 ATG9A 79065
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 AES 166
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 KRTAP5-4  
Two-hybrid Homo sapiens
7 TBC1D16  
Two-hybrid Homo sapiens
8 SMARCE1 6605
Two-hybrid Homo sapiens
9 ZNF439  
Two-hybrid Homo sapiens
10 SMARCD2 6603
Two-hybrid Homo sapiens
11 GNE 10020
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 EIF4E2  
Two-hybrid Homo sapiens
13 SBDS 51119
Affinity Capture-MS Homo sapiens
14 POMGNT2  
Two-hybrid Homo sapiens
15 EFNA3  
Two-hybrid Homo sapiens
16 LMO2  
Two-hybrid Homo sapiens
17 DBH 1621
Two-hybrid Homo sapiens
18 CRACR2A  
Two-hybrid Homo sapiens
19 C11orf87  
Two-hybrid Homo sapiens
20 DMRT3  
Two-hybrid Homo sapiens
21 MXD3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 CLEC18A  
Two-hybrid Homo sapiens
23 KRTAP2-4  
Two-hybrid Homo sapiens
24 NUDT3 11165
Affinity Capture-MS Homo sapiens
25 YIPF3 25844
Two-hybrid Homo sapiens
26 FTHL17  
Two-hybrid Homo sapiens
27 KRTAP10-11  
Two-hybrid Homo sapiens
28 SMOC1 64093
Two-hybrid Homo sapiens
29 NMU  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 KPRP 448834
Two-hybrid Homo sapiens
31 ICAM4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 LCE1A  
Two-hybrid Homo sapiens
33 LCE1B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 CST2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 MATN3  
Two-hybrid Homo sapiens
36 DNAL4  
Two-hybrid Homo sapiens
37 FASLG 356
Two-hybrid Homo sapiens
38 QPRT 23475
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 TSPAN4 7106
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 KRTAP12-1  
Two-hybrid Homo sapiens
41 PSMG2 56984
Two-hybrid Homo sapiens
42 DGKQ  
Two-hybrid Homo sapiens
43 BMP7 655
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 CDK5R1  
Two-hybrid Homo sapiens
45 TGIF2LY  
Affinity Capture-MS Homo sapiens
46 ZNF581  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 ITGB2 3689
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 ADAMTSL3 57188
Two-hybrid Homo sapiens
49 LRFN4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 KRTAP10-7  
Two-hybrid Homo sapiens
51 TRPV6  
Two-hybrid Homo sapiens
52 EPHB6  
Two-hybrid Homo sapiens
53 BCAM 4059
Two-hybrid Homo sapiens
54 ZNF672 79894
Two-hybrid Homo sapiens
55 NUBP2 10101
Two-hybrid Homo sapiens
56 LRCH4  
Two-hybrid Homo sapiens
57 C19orf66  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 INS 3630
Two-hybrid Homo sapiens
59 WT1-AS  
Two-hybrid Homo sapiens
60 KCNS2  
Two-hybrid Homo sapiens
61 XCL2  
Two-hybrid Homo sapiens
62 SPRY1 10252
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 HIST1H2AK 8330
Two-hybrid Homo sapiens
64 PVR 5817
Two-hybrid Homo sapiens
65 LCE4A  
Two-hybrid Homo sapiens
66 CARHSP1 23589
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 SLC25A6 293
Two-hybrid Homo sapiens
68 INPP5D 3635
Two-hybrid Homo sapiens
69 KCTD15 79047
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 KRTAP10-5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
71 CSF1 1435
Two-hybrid Homo sapiens
72 ZNF319  
Two-hybrid Homo sapiens
73 KRTAP3-1  
Two-hybrid Homo sapiens
74 STK16 8576
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
75 DOCK2 1794
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
76 TMEM57  
Two-hybrid Homo sapiens
77 RGL2 5863
Two-hybrid Homo sapiens
78 SLC5A5 6528
Two-hybrid Homo sapiens
79 MTA1 9112
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 FOXB1  
Two-hybrid Homo sapiens
81 EMC7 56851
Two-hybrid Homo sapiens
82 C5orf60  
Two-hybrid Homo sapiens
83 ZNF446  
Two-hybrid Homo sapiens
84 DHRS1 115817
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
85 RCHY1  
Two-hybrid Homo sapiens
86 CREB5 9586
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 CHIC2 26511
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 MXI1 4601
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 CYHR1  
Two-hybrid Homo sapiens
90 SPINK2  
Two-hybrid Homo sapiens
91 IL10 3586
Two-hybrid Homo sapiens
92 BLCAP  
Two-hybrid Homo sapiens
93 OLFM2  
Two-hybrid Homo sapiens
94 KRTAP5-3  
Two-hybrid Homo sapiens
95 TPMT 7172
Affinity Capture-MS Homo sapiens
96 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
97 GSTP1 2950
Two-hybrid Homo sapiens
98 GNMT 27232
Two-hybrid Homo sapiens
99 CRH  
Two-hybrid Homo sapiens
100 GTF3C5 9328
Two-hybrid Homo sapiens
101 LCE2C  
Two-hybrid Homo sapiens
102 SCNM1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
103 HSPA12B 116835
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
104 TMEFF2  
Two-hybrid Homo sapiens
105 SPG7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
106 HOXB9  
Two-hybrid Homo sapiens
107 LCE3C  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
108 SMCP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
109 TMEM8A 58986
Two-hybrid Homo sapiens
110 TMEM231 79583
Two-hybrid Homo sapiens
111 KRTAP10-10  
Two-hybrid Homo sapiens
112 SLC43A2 124935
Two-hybrid Homo sapiens
113 KRTAP4-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 TINAGL1 64129
Two-hybrid Homo sapiens
115 PTK7 5754
Two-hybrid Homo sapiens
116 CATSPER1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
117 F10 2159
Two-hybrid Homo sapiens
118 TMIE  
Two-hybrid Homo sapiens
119 PSMA1 5682
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
120 SDCBP 6386
Two-hybrid Homo sapiens
121 BCL6B  
Two-hybrid Homo sapiens
122 ADAMTSL4  
Two-hybrid Homo sapiens
123 AQP1 358
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
124 SLC25A10 1468
Two-hybrid Homo sapiens
125 LIMS2 55679
Two-hybrid Homo sapiens
126 ALDH16A1 126133
Two-hybrid Homo sapiens
127 LCE3A  
Two-hybrid Homo sapiens
128 CD164 8763
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 LCE3E  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
130 ALDH3B1 221
Two-hybrid Homo sapiens
131 KRTAP4-11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
132 PRKAA2 5563
Two-hybrid Homo sapiens
133 NMUR2  
Two-hybrid Homo sapiens
134 HBA1 3039
Two-hybrid Homo sapiens
135 SLC22A5 6584
Two-hybrid Homo sapiens
136 LINC00526  
Two-hybrid Homo sapiens
137 TPK1  
Affinity Capture-MS Homo sapiens
138 PCED1B  
Two-hybrid Homo sapiens
139 FZD10  
Two-hybrid Homo sapiens
140 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
141 TMEM41A  
Two-hybrid Homo sapiens
142 FAM90A1  
Two-hybrid Homo sapiens
143 HOXA1 3198
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
144 LIN7A 8825
Two-hybrid Homo sapiens
145 R3HDM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
146 CHRD  
Two-hybrid Homo sapiens
147 FAM74A4  
Two-hybrid Homo sapiens
148 KRTAP2-3  
Two-hybrid Homo sapiens
149 LYVE1  
Two-hybrid Homo sapiens
150 ZNF587  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
151 CST9L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
152 THEMIS2 9473
Two-hybrid Homo sapiens
153 DALRD3  
Two-hybrid Homo sapiens
154 CXCL5  
Two-hybrid Homo sapiens
155 KIF1A 547
Two-hybrid Homo sapiens
156 CA6 765
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
157 CCER1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
158 MFI2 4241
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
159 FAM71E2  
Two-hybrid Homo sapiens
160 TROAP  
Two-hybrid Homo sapiens
161 SHMT2 6472
Affinity Capture-RNA Homo sapiens
162 PDE9A  
Two-hybrid Homo sapiens
163 ZNF497  
Two-hybrid Homo sapiens
164 DAGLB 221955
Two-hybrid Homo sapiens
165 NAB2 4665
Two-hybrid Homo sapiens
166 NEDD9 4739
Two-hybrid Homo sapiens
167 MYPOP  
Two-hybrid Homo sapiens
168 ALPP 250
Two-hybrid Homo sapiens
169 CHRNG  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
170 GLRX3 10539
Two-hybrid Homo sapiens
171 VASN 114990
Two-hybrid Homo sapiens
172 LNX1  
Two-hybrid Homo sapiens
173 HBZ  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
174 MVP 9961
Two-hybrid Homo sapiens
175 KRTAP12-3  
Two-hybrid Homo sapiens
176 KLK8 11202
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
177 FRS3 10817
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
178 RPS19BP1  
Two-hybrid Homo sapiens
179 GATA2  
Two-hybrid Homo sapiens
180 GLP1R  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
181 PLSCR4  
Two-hybrid Homo sapiens
182 ZNF32  
Two-hybrid Homo sapiens
183 TRIM27  
Two-hybrid Homo sapiens
184 MAPKBP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
185 RET 5979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
186 ZNF414  
Two-hybrid Homo sapiens
187 TMEM180  
Two-hybrid Homo sapiens
188 PTPMT1 114971
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
189 FAM221A  
Two-hybrid Homo sapiens
190 RECK 8434
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
191 COL8A1 1295
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
192 PCSK5 5125
Two-hybrid Homo sapiens
193 CTSZ 1522
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
194 LHPP 64077
Affinity Capture-MS Homo sapiens
195 GALT 2592
Affinity Capture-MS Homo sapiens
196 UBQLN4 56893
Two-hybrid Homo sapiens
197 NIF3L1 60491
Affinity Capture-MS Homo sapiens
198 LCE1E  
Two-hybrid Homo sapiens
199 HSPBP1 23640
Two-hybrid Homo sapiens
200 GABRD  
Two-hybrid Homo sapiens
201 SPATA24  
Two-hybrid Homo sapiens
202 ZNF330  
Two-hybrid Homo sapiens
203 CHCHD3 54927
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
204 TRIM42  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
205 ALPI  
Two-hybrid Homo sapiens
206 SLC6A20  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
207 ZNF837  
Two-hybrid Homo sapiens
208 SLC22A23  
Two-hybrid Homo sapiens
209 ACY3 91703
Two-hybrid Homo sapiens
210 P2RY6  
Two-hybrid Homo sapiens
211 GNAI2 2771
Two-hybrid Homo sapiens
212 C16orf59 80178
Two-hybrid Homo sapiens
213 GIP  
Two-hybrid Homo sapiens
214 PID1 55022
Two-hybrid Homo sapiens
215 KRTAP9-8  
Two-hybrid Homo sapiens
216 MRPL40 64976
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
217 PGLS 25796
Two-hybrid Homo sapiens
218 C19orf57  
Two-hybrid Homo sapiens
219 PPAP2C 8612
Two-hybrid Homo sapiens
220 KRTAP13-4  
Two-hybrid Homo sapiens
221 STAC  
Two-hybrid Homo sapiens
222 LCE3D  
Two-hybrid Homo sapiens
223 KLHL38  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
224 WDR25  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
225 KRTAP9-3  
Two-hybrid Homo sapiens
226 HSD3B7 80270
Two-hybrid Homo sapiens
227 KRTAP10-3  
Two-hybrid Homo sapiens
228 NPBWR2  
Two-hybrid Homo sapiens
229 OTX1  
Two-hybrid Homo sapiens
230 KRTAP5-11  
Two-hybrid Homo sapiens
231 DGCR6  
Two-hybrid Homo sapiens
232 ITGB4 3691
Two-hybrid Homo sapiens
233 PTPN23 25930
Two-hybrid Homo sapiens
234 TXNDC5 81567
Two-hybrid Homo sapiens
235 HSPD1 3329
Two-hybrid Homo sapiens
236 ZNF417  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
237 TTPA  
Two-hybrid Homo sapiens