Gene description for ELANE
Gene name elastase, neutrophil expressed
Gene symbol ELANE
Other names/aliases ELA2
GE
HLE
HNE
NE
PMN-E
SCN1
Species Homo sapiens
 Database cross references - ELANE
ExoCarta ExoCarta_1991
Entrez Gene 1991
HGNC 3309
MIM 130130
UniProt P08246  
 ELANE identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for ELANE
Molecular Function
    endopeptidase activity GO:0004175 IDA
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    heparin binding GO:0008201 IDA
    cytokine binding GO:0019955 IPI
    RNA polymerase II transcription corepressor activity GO:0001106 IMP
    serine-type endopeptidase activity GO:0004252 IEA
    protease binding GO:0002020 IPI
Biological Process
    response to yeast GO:0001878 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    negative regulation of chemokine biosynthetic process GO:0045079 IDA
    proteolysis GO:0006508 IDA
    negative regulation of growth of symbiont in host GO:0044130 IEA
    negative regulation of interleukin-8 biosynthetic process GO:0045415 IDA
    negative regulation of chemotaxis GO:0050922 NAS
    positive regulation of MAP kinase activity GO:0043406 NAS
    defense response to bacterium GO:0042742 IDA
    positive regulation of interleukin-8 biosynthetic process GO:0045416 IDA
    negative regulation of inflammatory response GO:0050728 NAS
    neutrophil mediated killing of fungus GO:0070947 IEA
    phagocytosis GO:0006909 IEA
    response to lipopolysaccharide GO:0032496 IEA
    cellular calcium ion homeostasis GO:0006874 NAS
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    extracellular matrix disassembly GO:0022617 TAS
    protein catabolic process GO:0030163 NAS
    positive regulation of immune response GO:0050778 IEA
    collagen catabolic process GO:0030574 TAS
    extracellular matrix organization GO:0030198 TAS
    leukocyte migration GO:0050900 IEA
    acute inflammatory response to antigenic stimulus GO:0002438 IEA
    response to UV GO:0009411 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    secretory granule GO:0030141 IDA
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
    transcriptional repressor complex GO:0017053 IDA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified ELANE in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ELANE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLPI 6590
Invitro Homo sapiens
2 THBS1 7057
Invitro Homo sapiens
3 ELN  
Invitro Homo sapiens
4 SERPINA1 5265
Invitro Homo sapiens
5 ELANE 1991
Invitro Homo sapiens
Invitro Homo sapiens
6 SERPING1 710
Biochemical Activity Homo sapiens
7 NOTCH2NL  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 SERPINB1 1992
Invitro Homo sapiens
9 SERPINA3 12
Invitro Homo sapiens
Biochemical Activity Homo sapiens
10 SDC1 6382
Invitro Homo sapiens
11 SERPINF2 5345
Invitro Homo sapiens
Biochemical Activity Homo sapiens
12 KNG1 3827
Invitro Homo sapiens
View the network image/svg+xml



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