Gene description for MIB2
Gene name mindbomb E3 ubiquitin protein ligase 2
Gene symbol MIB2
Other names/aliases ZZANK1
ZZZ5
Species Homo sapiens
 Database cross references - MIB2
ExoCarta ExoCarta_142678
Entrez Gene 142678
HGNC 30577
MIM 611141
UniProt Q96AX9  
 MIB2 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for MIB2
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    signal transducer activity GO:0004871 IMP
    actin binding GO:0003779 IEA
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    protein ubiquitination GO:0016567 IEA
    Notch signaling pathway GO:0007219 IEA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IEA
    early endosome GO:0005769 IEA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified MIB2 in exosomes
1
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for MIB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTA1 58
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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