Gene description for NUPL1
Gene name nucleoporin like 1
Gene symbol NUPL1
Other names/aliases PRO2463
Species Homo sapiens
 Database cross references - NUPL1
ExoCarta ExoCarta_9818
Vesiclepedia VP_9818
Entrez Gene 9818
HGNC 20261
MIM 607615
UniProt Q9BVL2  
 NUPL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NUPL1
Molecular Function
    protein binding GO:0005515 IPI
    nuclear localization sequence binding GO:0008139 IBA
    structural constituent of nuclear pore GO:0017056 IBA
    identical protein binding GO:0042802 IEA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    nucleocytoplasmic transport GO:0006913 NAS
    protein transport GO:0015031 IEA
    regulation of protein import into nucleus GO:0042306 IEA
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 IBA
    nuclear pore GO:0005643 NAS
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified NUPL1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NUPL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Co-fractionation Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 NUPL2 11097
Affinity Capture-MS Homo sapiens
4 NUP214 8021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NUMA1 4926
Affinity Capture-MS Homo sapiens
6 NUP54 53371
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 ABI3 51225
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 KRT27 342574
Two-hybrid Homo sapiens
9 MAPRE1 22919
Affinity Capture-MS Homo sapiens
10 NUP205 23165
Affinity Capture-MS Homo sapiens
11 KIAA1191  
Affinity Capture-MS Homo sapiens
12 ILF3 3609
Co-fractionation Homo sapiens
13 Nup98  
Affinity Capture-MS Mus musculus
14 KRT33B 3884
Two-hybrid Homo sapiens
15 EEA1 8411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NUP62CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ABCC5 10057
Affinity Capture-MS Homo sapiens
18 NUP155 9631
Proximity Label-MS Homo sapiens
19 PKNOX2  
Two-hybrid Homo sapiens
20 XPO1 7514
Affinity Capture-MS Homo sapiens
21 UBQLN2 29978
Two-hybrid Homo sapiens
22 NUP62 23636
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 OSBPL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 APC  
Two-hybrid Homo sapiens
25 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MAPK3 5595
Two-hybrid Homo sapiens
27 SPSB4  
Affinity Capture-MS Homo sapiens
28 NUP188 23511
Affinity Capture-MS Homo sapiens
29 MIS18A  
Two-hybrid Homo sapiens
30 EGFR 1956
Negative Genetic Homo sapiens
31 KRT28 162605
Two-hybrid Homo sapiens
32 COG2 22796
Affinity Capture-MS Homo sapiens
33 RNF40 9810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NUP35 129401
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SPATA1  
Affinity Capture-MS Homo sapiens
36 NUP98 4928
Affinity Capture-MS Homo sapiens
37 GDF15 9518
Affinity Capture-MS Homo sapiens
38 TRIM54  
Two-hybrid Homo sapiens
39 COG6 57511
Two-hybrid Homo sapiens
40 NTPCR 84284
Affinity Capture-MS Homo sapiens
41 HTT 3064
Two-hybrid Homo sapiens
42 MEIS2  
Two-hybrid Homo sapiens
43 NUP107 57122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 Nup107  
Affinity Capture-MS Mus musculus
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 NDC1 55706
Affinity Capture-MS Homo sapiens
47 VCP 7415
Affinity Capture-MS Homo sapiens
48 TOP1MT  
Affinity Capture-MS Homo sapiens
49 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 PHF21A  
Affinity Capture-MS Homo sapiens
51 DTX2 113878
Proximity Label-MS Homo sapiens
52 AGR2 10551
Two-hybrid Homo sapiens
53 COG3 83548
Two-hybrid Homo sapiens
54 MIB2 142678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 KRAS 3845
Negative Genetic Homo sapiens
56 RANBP1 5902
Affinity Capture-MS Homo sapiens
57 EHBP1L1 254102
Affinity Capture-MS Homo sapiens
58 NUP93 9688
Affinity Capture-MS Homo sapiens
59 OBSL1 23363
Affinity Capture-MS Homo sapiens
60 DMD 1756
Affinity Capture-MS Homo sapiens
61 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 UBQLN1 29979
Two-hybrid Homo sapiens
63 GDF3 9573
Affinity Capture-MS Homo sapiens
64 PKNOX1  
Two-hybrid Homo sapiens
65 LAMTOR3 8649
Co-fractionation Homo sapiens
66 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 RANBP2 5903
Affinity Capture-MS Homo sapiens
68 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ACBD5 91452
Proximity Label-MS Homo sapiens
70 TRAF1 7185
Two-hybrid Homo sapiens
71 RAN 5901
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUPL1 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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