Gene description for NUP62
Gene name nucleoporin 62kDa
Gene symbol NUP62
Other names/aliases IBSN
SNDI
p62
Species Homo sapiens
 Database cross references - NUP62
ExoCarta ExoCarta_23636
Vesiclepedia VP_23636
Entrez Gene 23636
HGNC 8066
MIM 605815
UniProt P37198  
 NUP62 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NUP62
Molecular Function
    ubiquitin binding GO:0043130 ISS
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 ISS
    receptor signaling complex scaffold activity GO:0030159 ISS
    chromatin binding GO:0003682 NAS
    nucleocytoplasmic transporter activity GO:0005487 NAS
    thyroid hormone receptor binding GO:0046966 ISS
    PTB domain binding GO:0051425 IEA
    structural constituent of nuclear pore GO:0017056 IEA
Biological Process
    transcription, DNA-templated GO:0006351 ISS
    transmembrane transport GO:0055085 TAS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    viral transcription GO:0019083 TAS
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IEA
    negative regulation of MAP kinase activity GO:0043407 IEA
    regulation of glucose transport GO:0010827 TAS
    gene expression GO:0010467 TAS
    hormone-mediated signaling pathway GO:0009755 NAS
    regulation of cellular response to heat GO:1900034 TAS
    regulation of Ras protein signal transduction GO:0046578 NAS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of programmed cell death GO:0043069 ISS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISS
    cellular response to heat GO:0034605 TAS
    cell aging GO:0007569 IEA
    mitotic nuclear envelope disassembly GO:0007077 TAS
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 NAS
    protein transport GO:0015031 IEA
    protein sumoylation GO:0016925 TAS
    cellular protein metabolic process GO:0044267 TAS
    cell death GO:0008219 ISS
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of cell proliferation GO:0008285 ISS
    carbohydrate metabolic process GO:0005975 TAS
    hexose transport GO:0008645 TAS
    cell surface receptor signaling pathway GO:0007166 NAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    small molecule metabolic process GO:0044281 TAS
    post-translational protein modification GO:0043687 TAS
    regulation of signal transduction GO:0009966 NAS
    viral process GO:0016032 TAS
    nucleocytoplasmic transport GO:0006913 NAS
    mRNA transport GO:0051028 IEA
    glucose transport GO:0015758 TAS
    viral life cycle GO:0019058 TAS
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    Flemming body GO:0090543 IDA
    nuclear membrane GO:0031965 IDA
    spindle pole GO:0000922 IEA
    pore complex GO:0046930 NAS
    ribonucleoprotein complex GO:0030529 IDA
    nuclear pore GO:0005643 ISS
    cytoplasm GO:0005737 NAS
    nuclear envelope GO:0005635 TAS
    nucleocytoplasmic shuttling complex GO:0031074 NAS
 Experiment description of studies that identified NUP62 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NUP62
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PBX2  
Affinity Capture-MS Homo sapiens
2 NXF2  
Reconstituted Complex Homo sapiens
3 NFX1  
Reconstituted Complex Homo sapiens
4 XPO6 23214
Two-hybrid Homo sapiens
5 NUP62 23636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KPNB1 3837
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
7 NUP54 53371
Two-hybrid Homo sapiens
8 CCDC53 51019
Two-hybrid Homo sapiens
9 NUPL1 9818
Affinity Capture-MS Homo sapiens
10 NUTF2 10204
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
11 GORASP2 26003
Two-hybrid Homo sapiens
12 IPO5 3843
Invitro Homo sapiens
Invivo Homo sapiens
13 THAP1  
Two-hybrid Homo sapiens
14 GTF2E2  
Invitro Homo sapiens
15 DTNB 1838
Two-hybrid Homo sapiens
16 KPNA1 3836
Reconstituted Complex Homo sapiens
17 HSF2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
18 PTMA 5757
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
19 XPO1 7514
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 IK 3550
Two-hybrid Homo sapiens
21 TRAF3 7187
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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